Western blotting: Sample preparation to detection

Journal of Visualized Experiments

Volumn , Issue 44, 2010, Pages

  Eslami, Anna ^a   Lujan, Jesse ^a  

^a EMD Chemicals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355141948     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2359     Document Type: Article

References (6)

1. Towbin, H., Staehelin, T., & Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76 (9): 4350-4354 (1979).
2. Renart, J., Reiser, J., & Stark, G.R. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A 76 (7): 3116-3120 (1979).
3. Burnette, W.N. 'Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry (United States: Academic Press) 112 (2): 195-203 (1981).
4. Ambroz, K. Improving quantification accuracy for Western blots Image Analysis 09/2006. (2006).
5. Quan, Zhiwei, et al. Reactive oxygen species-mediated endoplasmic reticulum stress and mitochondrial dysfunction contribute to cirsimartitin-induced apoptosis in human gallbladder carcinoma GBC-SD cells. Cancer Lett Article in Press (2010).
6. O'Malley, Dervia et al. "Alterations in colonic corticotrophin-releasing factor receptors in the maternally separated rat model of irritable bowel syndrome: Differential effects of acute psychological and physical stressors". Peptides 31 (4): 662-670 (2010).