Direct observation of enzymes replicating DNA using a single-molecule DNA stretching assay

Journal of Visualized Experiments

Volumn , Issue 37, 2010, Pages

  Kulczyk, Arkadiusz W ^a   Tanner, Nathan A ^a   Loparo, Joseph J ^a   Richardson, Charles C ^a   van Oijen, Antoine M ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Biophysics; Cellular biology; DNA polymerase; DNA replication; Issue 37; Single molecule

Indexed keywords

EID: 80355140848     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1689     Document Type: Article

References (6)

1. Lee, J. B. et al. DNA primase acts as a molecular brake in DNA replication. Nature 439, 621-624 (2006).
2. Tanner, N. A. & van Oijen, A. M. Single-molecule observation of prokaryotic DNA replication. Methods Mol Biol 521, 397-410 (2009).
3. Sofia, S. J., Premnath, V. V. & Merrill, E. W. Poly(ethylene oxide) Grafted to Silicon Surfaces: Grafting Density and Protein Adsorption. Macromolecules 31, 5059-5070 (1998).
4. Tanner, N. A., Loparo, J. J. & van Oijen, A. M. Visualizing single-molecule DNA replication with fluorescence microscopy. Journal of Visual Experiments (2009).
5. Hamdan, S. M., Loparo, J. J., Takahashi, M., Richardson, C. C. & van Oijen, A. M. Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis. Nature 457, 336-339 (2009).
6. van Oijen, A. M. et al. Single-molecule kinetics of lambda exonuclease reveal base dependence and dynamic disorder. Science 301, 1235-1238 (2003).