Visualizing single-molecule DNA replication with fluorescence microscopy

Journal of Visualized Experiments

Volumn , Issue 32, 2009, Pages

  Tanner, Nathan A ^a   Loparo, Joseph J ^a   van Oijen, Antoine M ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355137819     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1529     Document Type: Article

References (6)

1. Tanner, N.A. et al. Real-time single-molecule observation of rolling-circle DNA replication. Nucleic Acids Res 37, e27 (2009).
2. Tabor, S., Huber, H.E. & Richardson, C.C. Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage t7. J Biol Chem 262, 16212-16223 (1987).
3. Sofia, S.J., Premnath, V.V. & Merrill, E.W. Poly(ethylene oxide) grafted to silicon surfaces: Grafting density and protein adsorption. Macromolecules 31, 5059-5070 (1998).
4. Tanner, N.A. & van Oijen, A.M. Single-molecule observation of prokaryotic DNA replication. in DNA replication: Methods and protocols, Vol. 521 (eds. Vengrova, S. & J.Z., D.) 397-410 (Humana Press, New York, 2009).
5. Hamdan, S.M., Loparo, J.J., Takahashi, M., Richardson, C.C. & van Oijen, A.M. Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis. Nature 457, 336-339 (2009).
6. Heller, R.C. & Marians, K.J. Replication fork reactivation downstream of a blocked nascent leading strand. Nature 439, 557-562 (2006).