Isolation of protoplasts from tissues of 14-day-old seedlings of Arabidopsis thaliana

Journal of Visualized Experiments

Volumn , Issue 30, 2009, Pages

  Zhai, Zhiyang ^a   Jung, Ha Il ^a   Vatamaniuk, Olena K ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Arabidopsis; Isolation; Issue 30; Plant Biology; Protoplasts; Seedlings

Indexed keywords

EID: 80355138514     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1149     Document Type: Article

References (5)

1. Cocking, E. C. A Method for the Isolation of Plant Protoplasts and Vacuoles. Nature 187, 962-963 (1960).
2. Chen, S. & Halkier, B. A. Characterization of glucosinolate uptake by leaf protoplasts of Brassica napus. J Biol Chem 275, 22955-60 (2000).
3. Robert, S., Zouhar, J., Carter, C. & Raikhel, N. Isolation of intact vacuoles from Arabidopsis rosette leaf-derived protoplasts. Nat. Protocols 2, 259-262 (2007).
4. Yoo, S. D., Cho, Y. H. & Sheen, J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2, 1565-72 (2007).
5. Zhai, Z., Sooksa-nguan, T. and Vatamaniuk, O.K. Establishing RNAi as a reverse genetic approach for gene functional analysis in protoplasts. Plant Phys., 149 (2), 642-652 (2009).