Modified annexin V/propidium iodide apoptosis assay for accurate assessment of cell death

Journal of Visualized Experiments

Volumn , Issue 50, 2011, Pages

  Rieger, Aja M ^a   Nelson, Kimberly L ^a   Konowalchuk, Jeffrey D ^a   Barreda, Daniel R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355130039     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2597     Document Type: Article

References (12)

1. Vermes, I., Haanen, C., Steffens-Nakken, H., & Reutelingsperger, C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V. J Immunol Methods 184, 39-51 (1995).
2. Vermes, I., Haanen, C., & Reutelingsperger, C. Flow cytometry of apoptotic cell death. J Immunol Methods 243, 167-190 (2000).
3. Cornelissen, M., Philippe, J., De Sitter, S., & De Ridder, L. Annexin V expression in apoptotic peripheral blood lymphocytes: An electron microscopic evaluation. Apoptosis 7, 41-47 (2002).
4. Fried, J., Perez, A.G., & Clarkson, B.D. Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data. J Cell Biol 71, 172-181 (1976).
5. Bacso, Z., Everson, R.B., & Eliason, J.F. The DNA of Annexin V-binding apoptotic cells is highly fragmented. Cancer Res 60, 4623-4628 (2000).
6. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lassota, P., & Traganos, F. Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795-808 (1992).
7. Kroemer, G., Dallaporta, B., & Resche-Rigon, M. The mitochondrial death/life regulator in apoptosis and necrosis. Annu. Rev. Physiol. 60, 619-642 (1998).
8. Denecker, G., Vercammen, D., Declercq, W., & Vandenabeele, P. Apoptotic and necrotic cell death induced by death domain receptors. Cell Mol. Life Sci. 58, 356-370 (2001).
9. Faleiro, L. & Lazebnik, Y. Caspases disrupt the nuclear-cytoplasmic barrier. J Cell Biol 151, 951-959 (2000).
10. Rieger, A.M., Hall, B.E., Luong, L.T., Schang, L.M., & Barreda, D.R. Conventional apoptosis assays using propidium iodide generate a significant number of false positives that prevent accurate assessment of cell death. J Immunol Methods 358, 81-92 (2010).
11. Edward, R. Minireview: Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells. Mol. Cells 27, 391-396 (2009).
12. Njoh, K.L., Patterson, L.H., Zloh, M., Wiltshire, M., Fisher, J., Chappell, S., Ameer-Beg, S., Bai, Y., Matthews, D., Errington, R.J., & Smith, P.J. Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells. Cytometry Part A 69, 805-814 (2006).