Conventional apoptosis assays using propidium iodide generate a significant number of false positives that prevent accurate assessment of cell death

Journal of Immunological Methods

Volumn 358, Issue 1-2, 2010, Pages 81-92

  Rieger, Aja M ^a   Hall, Brian E ^b   Luong, Le Thuong ^a   Schang, Luis M ^a   Barreda, Daniel R ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b Amnis Corporation   (United States)

Author keywords

Apoptosis; Cell death; Necrosis; Propidium iodide; RNA; Viral infection

Indexed keywords

PROPIDIUM IODIDE; RNA; VIRUS RNA;

ACCURACY; ANIMAL CELL; APOPTOSIS; ARTICLE; BLASTODERM; CELL DEATH; CONTROLLED STUDY; CYTOPLASM; EMBRYO; FALSE POSITIVE RESULT; FEMALE; FLOW CYTOMETRY; GOLDFISH; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; LEUKOCYTE; MACROPHAGE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; STAINING; SWINE; VERO CELL; VIRUS CELL INTERACTION;

ANIMALS; ANTHRAQUINONES; APOPTOSIS; CELL DEATH; CELL LINE; CELL LINE, TUMOR; CELL NUCLEUS; CELL SURVIVAL; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CHICK EMBRYO; CYTOPLASM; DNA; FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; FLUORESCENT DYES; FORMALDEHYDE; GOLDFISH; HUMANS; MICE; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; PROPIDIUM; RIBONUCLEASES; RNA, DOUBLE-STRANDED; STAINING AND LABELING; SUS SCROFA; TISSUE FIXATION; VERO CELLS; VIRUS REPLICATION;

ANIMALIA; AVES; MUS; SUIDAE; TELEOSTEI;

EID: 77953290062     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.03.019     Document Type: Article

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