Modeling neural immune signaling of episodic and chronic migraine using spreading depression in vitro

Journal of Visualized Experiments

Volumn , Issue 52, 2011, Pages

  Pusic, Aya D ^a   Grinberg, Yelena Y ^a   Mitchell, Heidi M ^a   Kraig, Richard P ^a  

^a UNIVERSITY OF CHICAGO MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355129693     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2910     Document Type: Article

References (17)

1. Kunkler, P.E. & Kraig, R.P. Calcium waves precede electrophysiological changes of spreading depression in hippocampal organ cultures. J Neurosci 18(9), 3416-3425 (1998).
2. Kunkler, P.E., Hulse, R.E., Schmitt, M.W., Nicholson, C. & Kraig, R.P. Optical current source density analysis in hippocampal organotypic culture shows that spreading depression occurs with uniquely reversing currents. J Neurosci 25 (15), 3952-3961 (2005).
3. Hulse, R.E., Swenson, W.G., Kunkler, P.E., White, D.M. & Kraig, R.P. Monomeric IgG is neuroprotective via enhancing microglial recycling endocytosis and TNF-αlpha. J Neurosci 28 (47), 12199-12211 (2008).
4. Hailer, N.P., Jarhult, J.D. & Nitsch, R. Resting microglial cells in vitro: analysis of morphology and adhesion molecule expression in organotypic hippocampal slice cultures. Glia 18 (4), 319-331 (1996).
5. Ransohoff, R.M. & Perry, V.H. Microglial physiology: unique stimuli, specialized responses. Annu Rev Immunol 27, 119-145 (2009).
6. Mitchell, H.M., White, D.M., Domowicz, M.S. & Kraig, R.P. Cold pre-conditioning neuroprotection depends on TNF-αlpha and is enhanced by blockade of interleukin-11. J Neurochem DOI: 10.1111/j.1471-4159.2010.07103.x (2010).
7. Mitchell, H.M., White, D.M., & Kraig, R.P. Strategies for study of neuroprotection from cold-preconditioning. J Vis Exp (43) pii: 2192. doi:10.3791/2192 (2010).
8. Kunkler, P.E. & Kraig, R.P. Reactive astrocytosis from excitotoxic injury in hippocampal organ culture parallels that seen in vivo. J Cereb Blood Flow Metab 17 (1), 26-43 (1997).
9. Grinberg, Y.Y., Milton, J.G. & Kraig, R.P. Spreading depression sends microglia on Lévy flights. PLoS ONE 6(4), e19294 doi:10.1371/journal.pone 0019294 (2011).
10. Koroleva, V.I., Oitzl, M.S. & Bures, J. Threshold of synaptically elicited cortical spreading depression: drug-induced changes in rats. Electroencephalogr Clin Neurophysiol 60 (1), 55-64 (1985).
11. Gubern, C., et al. Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia. BMC Mol Biol 10, 57 (2009).
12. Tian, Y.F., et al. The quantification of ADAMTS expression in an animal model of cerebral ischemia using real-time PCR. Acta Anaesthesiol Scand 51 (2), 158-164 (2007).
13. Pfaffl, M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29 (9), e45 (2001).
14. Bustin, S.A., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55 (4), 611-622 (2009).
15. Pusic, A.D., & Kraig, R.P. Inflammatory gene micro array profiling demonstrates T-cell-like activation after recurrent spreading depression - implications for migraine pathogenesis. Soc Neurosci abst 36: Prog #346.2 (2010).
16. Quinn, B. & Graybiel, A.M. A differentiated silver intensification procedure for the peroxidase-diaminobenzidine reaction. J Histochem Cytochem 44 (1), 71-74 (1996).
17. Caggiano, A.O. & Kraig, R.P. Eicosanoids and nitric oxide influence induction of reactive gliosis from spreading depression in microglia but not astrocytes. J Comp Neurol 369 (1), 93-108 (1996).