Fluorescence recovery after photobleaching (FRAP) of fluorescence tagged proteins in dendritic spines of cultured hippocampal neurons

Journal of Visualized Experiments

Volumn , Issue 50, 2011, Pages

  Zheng, Chan Ying ^a   Petralia, Ronald S ^a   Wang, Ya Xian ^a   Kachar, Bechara ^a  

^a NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355128031     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2568     Document Type: Article

References (6)

1. Zheng, C. Y., Petralia, R. S., Wang, Y. X., Kachar, B. & Wenthold, R. J. SAP102 is a highly mobile MAGUK in spines. J Neurosci 30, 4757-4766, (2010).
2. Grati, M. et al. Rapid turnover of stereocilia membrane proteins: evidence from the trafficking and mobility of plasma membrane Ca(2+)-ATPase 2. J Neurosci 26, 6386-6395, (2006).
3. Liu, L. N., Aartsma, T. J., Thomas, J. C., Zhou, B. C., & Zhang, Y. Z. FRAP Analysis on Red Alga Reveals the Fluorescence Recovery Is Ascribed to Intrinsic Photoprocesses of Phycobilisomes than Large-Scale Diffusion. PLoS ONE 4, 5295, (2009).
4. Wiedenmann, J. & Nienhaus, G. U. Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family. Expert Rev Proteomics 3, 361-374, (2006).
5. Shaner, N. C., Patterson, G. H. & Davidson, M. W. Advances in fluorescent protein technology. J Cell Sci 120, 4247-4260, (2007).
6. Sprague, B. L. & McNally, J. G. FRAP analysis of binding: proper and fitting. Trends Cell Biol 15, 84-91, (2005).