Single cell transfection in chick embryos

Journal of Visualized Experiments

Volumn , Issue 43, 2010, Pages

  Ben Yair, Raz ^a   Kalcheim, Chaya ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Avian embryo; Developmental Biology; Electroporation; Gene miss expression; Issue 43; Lineage tracing; Micro injection; Neural tube; Single cell transfection; Somite

Indexed keywords

EID: 80355124263     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2133     Document Type: Article

References (9)

1. Ben-Yair, R. & Kalcheim, C. Lineage analysis of the avian dermomyotome sheet reveals the existence of single cells with both dermal and muscle progenitor fates. Development 132, 689-701 (2005).
2. Ben-Yair, R. & Kalcheim, C. Notch and bone morphogenetic protein differentially act on dermomyotome cells to generate endothelium, smooth, and striated muscle. J Cell Biol 180, 607-618 (2008).
3. Cinnamon, Y., Kahane, N. & Kalcheim, C. Characterization of the early development of specific hypaxial muscles from the ventrolateral myotome. Development. 126, 4305-4315 (1999).
4. Stark, D.A. & Kulesa, P.M. Photoactivatable green fluorescent protein as a single-cell marker in living embryos. Dev Dyn 233, 983-992 (2005).
5. Haas, K., Jensen, K., Sin, W.C., Foa, L. & Cline, H.T. Targeted electroporation in Xenopus tadpoles in vivo--from single cells to the entire brain. Differentiation 70, 148-154 (2002).
6. Haas, K., Sin, W.C., Javaherian, A., Li, Z. & Cline, H.T. Single-cell electroporation for gene transfer in vivo. Neuron 29, 583-591 (2001).
7. Hewapathirane, D.S. & Haas, K. Single cell electroporation in vivo within the intact developing brain. J Vis Exp (2008).
8. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87-90 (2002).
9. Sato, Y. et al. Stable integration and conditional expression of electroporated transgenes in chicken embryos. Dev Biol 305, 616-624 (2007).