Single cell electroporation in vivo within the intact developing brain

Journal of Visualized Experiments

Volumn , Issue 17, 2008, Pages

  Hewapathirane, D Sesath ^a   Haas, Kurt ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Electroporation; Fluorescence labeling; Gene delivery; Issue 17; Micropipette; Neuronal imaging; Neuroscience; Transfection

Indexed keywords

EID: 80055117198     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/705     Document Type: Article

References (4)

1. Bestman JE, Ewald RC, Chiu SL, Cline HT. In vivo single-cell electroporation for transfer of DNA and macromolecules. Nature Protocols. 2006;1(3):1267-72.
2. Dunfield D, Haas K. Single cell electroporation. In: Encyclopedia of Neuroscience (4th Ed.) Elsevier, Amsterdam. (in press)
3. Haas K, Jensen K, Sin WC, Foa L, Cline HT. Targeted electroporation in Xenopus tadpoles in vivo--from single cells to the entire brain. Differentiation. 2002 Jun;70(4-5):148-54.
4. Haas K, Sin WC, Javaherian A, Li Z, Cline HT. Single-cell electroporation for gene transfer in vivo. Neuron. 2001 Mar;29(3):583-91.