Using spin-label electron paramagnetic resonance (EPR) to discriminate and characterize the cholesterol bilayer domain

Chemistry and Physics of Lipids

Volumn 164, Issue 8, 2011, Pages 819-829

  Raguz, Marija ^a,b   Mainali, Laxman ^a   Widomska, Justyna ^c   Subczynski, Witold K ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b UNIVERSITY OF SPLIT   (Croatia)

^c MEDICAL UNIVERSITY OF LUBLIN   (Poland)

Author keywords

Cholesterol; Cholesterol bilayer domain; EPR; Fiber cell; Lens lipids; Membrane; Spin label

Indexed keywords

2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; ANDROSTANE DERIVATIVE; CHOLESTANE DERIVATIVE; CHOLESTEROL; CHOLESTEROL 1 PALMITOYL 2 OLEOYLPHOSPHATIDYLCHOLINE; OXYGEN; UNCLASSIFIED DRUG; WATER;

ANDROSTANE SPIN LABELING; ARTICLE; CHEMICAL STRUCTURE; CHOLESTANE SPIN LABELING; CHOLESTEROL BILAYER DOMAIN; ELECTRON SPIN RESONANCE; HYDROPHOBICITY; LIPID BILAYER; MEMBRANE PERMEABILITY; NONHUMAN; OXYGEN TRANSPORT; PRIORITY JOURNAL; SOLUBILITY; SPIN LABELING;

BIOLOGICAL TRANSPORT; CHOLESTEROL; ELECTRON SPIN RESONANCE SPECTROSCOPY; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LIPID BILAYERS; OXYGEN; PHOSPHATIDYLCHOLINES; SPIN LABELS; TEMPERATURE;

EID: 80255137179     PISSN: 00093084     EISSN: 18732941     Source Type: Journal    
DOI: 10.1016/j.chemphyslip.2011.08.001     Document Type: Article

References (46)

1. D. Bach Differential scanning calorimetric study of mixtures of cholesterol with phosphatidylserine or galactocerebroside Chemistry and Physics of Lipids 35 1984 385 392
2. D. Bach, N. Borochov, and E. Wachtel Phase separation of cholesterol from phosphatidylserine-cholesterol mixtures in the presence of the local anesthetic tetracaine Chemistry and Physics of Lipids 130 2004 99 107 (Pubitemid 38681194)
3. D. Bach, and E. Wachtel Phospholipid/cholesterol model membranes: formation of cholesterol crystallites Biochimica et Biophysica Acta 1610 2003 187 197 (Pubitemid 36324463)
4. C.R. Benatti, M.T. Lamy, and R.M. Epand Cationic amphiphiles and the solubilization of cholesterol crystallites in membrane bilayers Biochimica et Biophysica Acta 1778 2008 844 853
5. N. Borochov, E.J. Wachtel, and D. Bach Phase behavior of mixtures of cholesterol and saturated phosphatidylglycerols Chemistry and Physics of Lipids 76 1995 85 92
6. M. Bourges, D.M. Small, and D.G. Dervichian Biophysics of lipidic associations. II. The ternary systems: cholesterol-lecithin-water Biochimica et Biophysica Acta 137 1967 157 167
7. J.T. Buboltz A more efficient device for preparing model-membrane liposomes by the rapid solvent exchange method Review of Scientific Instruments 80 2009 124301
8. D.A. Cadenhead, and F. Muller-Landau Molecular packing in steroid-lecithin monolayers part III:mixed films of 3-doxyl cholestane and 3-doxyl-17-hydroxyl-androstane with dipalmitoylphosphatidylcholine Chemistry and Physics of Lipids 25 1979 329 343
9. J.J. Cheetham, E. Wachtel, D. Bach, and R.M. Epand Role of the stereochemistry of the hydroxyl group of cholesterol and the formation of nonbilayer structures in phosphatidylethanolamines Biochemistry 28 1989 8928 8934 (Pubitemid 19278556)
10. K.A. Earle, J.K. Moscicki, M. Ge, D.E. Budil, and J.H. Freed 250-GHz electron spin resonance studies of polarity gradients along the aliphatic chains in phospholipid membranes Biophysical Journal 66 1994 1213 1221 (Pubitemid 24106368)
11. R.M. Epand Cholesterol in bilayers of sphingomyelin or dihydrosphingomyelin at concentrations found in ocular lens membranes Biophysical Journal 84 2003 3102 3110 (Pubitemid 36531758)
12. R.M. Epand, D.W. Hughes, B.G. Sayer, N. Borochov, D. Bach, and E. Wachtel Novel properties of cholesterol-dioleoylphosphatidylcholine mixtures Biochimica et Biophysica Acta 1616 2003 196 208 (Pubitemid 38366272)
13. W. Guo, and J.A. Hamilton 13C MAS NMR studies of crystalline cholesterol and lipid mixtures modeling atherosclerotic plaques Biophysical Journal 71 1996 2857 2868 (Pubitemid 26367748)
14. J. Huang, J.T. Buboltz, and G.W. Feigenson Maximum solubility of cholesterol in phosphatidylcholine and phosphatidylethanolamine bilayers Biochimica et Biophysica Acta 1417 1999 89 100 (Pubitemid 29078589)
15. J.S. Hyde, and W.K. Subczynski Spin-label oximetry L.J. Berliner, J. Reuben, Biological Magnetic Resonance 1989 Plenum Press New York 399 425
16. K. Kawasaki, J.-J. Yin, W.K. Subczynski, J.S. Hyde, and A. Kusumi Pulse EPR detection of lipid exchange between protein rich raft and bulk domains in the membrane: methodology development and its application to studies of influenza viral membrane Biophysical Journal 80 2001 738 748 (Pubitemid 32128327)
17. W. Knoll, G. Schmidt, K. Ibel, and E. Sackmann Small-angle neutron scattering study of lateral phase separation in dimyristoylphosphatidylcholine- cholesterol mixed membranes Biochemistry 24 1985 5240 5246
18. D. Kurad, G. Jeschke, and D. Marsh Lipid membrane polarity profiles by high-field EPR Biophysical Journal 85 2003 1025 1033 (Pubitemid 36909666)
19. A. Kusumi, W.K. Subczynski, and J.S. Hyde Oxygen transport parameter in membranes as deduced by saturation recovery measurements of spin-lattice relaxation times of spin labels Proceedings of the National Academy of Sciences of the United States of America 79 1982 1854 1858 (Pubitemid 12088526)
20. A. Kusumi, W.K. Subczynski, M. Pasenkiewicz-Gierula, J.S. Hyde, and H. Merkle Spin-label studies on phosphatidylcholine-cholesterol membranes: effects of alkyl chain length and unsaturation in the fluid phase Biochimica et Biophysica Acta 854 1986 307 317 (Pubitemid 16149085)
21. S.L. Laiken, and M.P. Printz Kinetic class analysis of hydrogen-exchange data Biochemistry 9 1970 1547 1553
22. C.R. Loomis, G.G. Shipley, and D.M. Small The phase behavior of hydrated cholesterol Journal of Lipid Research 20 1979 525 535
23. F. Muller-Landau, and D.A. Cadenhead Molecular packing in steroid-lecitin monolayers. Part I. Pure films of cholesterol, 3-doxyl-cholestane, 3-doxyl-17-hydroxyl-androstane, tetradecanoic acid and dipalmitoylphosphatidylcholine Chemistry and Physics of Lipids 25 1979 299 314
24. F. Muller-Landau, and D.A. Cadenhead Molecular packing in steroid-lecitin monolayers. Part II. Mixed films of cholesterol with dipalmitoylphosphatidylcholine and tetradecanoic acid Chemistry and Physics of Lipids 25 1979 315 328
25. M. Pasenkiewicz-Gierula, W.E. Antholine, W.K. Subczynski, O. Baffa, and J.S. Hyde Assesment of the ESR spectra of CuKTSM2 Inorganic Chemistry 26 1987 3945 3949
26. M. Pasenkiewicz-Gierula, W.K. Subczynski, and A. Kusumi Rotational diffusion of a steroid molecule in phosphatidylcholine-cholesterol membranes: fluid-phase microimmiscibility in unsaturated phosphatidylcholine-cholesterol membranes Biochemistry 29 1990 4059 4069 (Pubitemid 20145640)
27. V. Pata, and N. Dan Effect of membrane characteristics on phase separation and domain formation in cholesterol-lipid mixtures Biophysical Journal 88 2005 916 924 (Pubitemid 40975927)
28. R. Preston Mason, T.N. Tulenko, and R.F. Jacob Direct evidence for cholesterol crystalline domains in biological membranes: role in human pathobiology Biochimica et Biophysica Acta 1610 2003 198 207 (Pubitemid 36324464)
29. M. Raguz, L. Mainali, J. Widomska, and W.K. Subczynski The immiscible cholesterol bilayer domain exists as an integral part of phospholipid bilayer membranes Biochimica et Biophysica Acta 1808 2011 1072 1080
30. M. Raguz, J. Widomska, J. Dillon, E.R. Gaillard, and W.K. Subczynski Characterization of lipid domains in reconstituted porcine lens membranes using EPR spin-labeling approaches Biochimica et Biophysica Acta 1778 2008 1079 1090
31. M. Raguz, J. Widomska, J. Dillon, E.R. Gaillard, and W.K. Subczynski Physical properties of the lipid bilayer membrane made of cortical and nuclear bovine lens lipids: EPR spin-labeling studies Biochimica et Biophysica Acta 1788 2009 2380 2388
32. S. Schreier, C.F. Polnaszek, and I.O. Smith Spin labels in membranes. Problems in practice Biochimica et Biophysica Acta 515 1978 395 436 (Pubitemid 9099586)
33. W.K. Subczynski, C.C. Felix, C.S. Klug, and J.S. Hyde Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators Journal of Magnetic Resonance 176 2005 244 248 (Pubitemid 41336693)
34. W.K. Subczynski, and J.S. Hyde Concentration of oxygen in lipid bilayers using a spin-label method Biophysical Journal 41 1983 283 286
35. W.K. Subczynski, J.S. Hyde, and A. Kusumi Oxygen permeability of phosphatidylcholine-cholesterol membranes Proceedings of the National Academy of Sciences of the United States of America 86 1989 4474 4478 (Pubitemid 19164577)
36. W.K. Subczynski, J.S. Hyde, and A. Kusumi Effect of alkyl chain unsaturation and cholesterol intercalation on oxygen transport in membranes: a pulse ESR spin labeling study Biochemistry 30 1991 8578 8590
37. W.K. Subczynski, M. Raguz, and J. Widomska Studying lipid organization in biological membranes using liposomes and EPR spin labeling V. Weissig, Liposomes. Methods and Protocols, 2010 ed. 2010 Humana Press Glendale, AZ 247 269
38. W.K. Subczynski, J. Widomska, A. Wisniewska, and A. Kusumi Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains T.J. Rafts McIntosh, Methods in Molecular Biology, Lipid 2007 Humana Press Totowa 143 157
39. W.K. Subczynski, A. Wisniewska, J.-J. Yin, J.S. Hyde, and A. Kusumi Hydrophobic barriers of lipid bilayer membranes formed by reduction of water penetration by alkyl chain unsaturation and cholesterol Biochemistry 33 1994 7670 7681 (Pubitemid 24230593)
40. M.J. Swamy, L. Ciani, M. Ge, A.K. Smith, D. Holowka, B. Baird, and J.H. Freed Coexisting domains in the plasma membranes of live cells characterized by spin-label ESR spectroscopy Biophysical Journal 90 2006 4452 4465 (Pubitemid 43830891)
41. G.M. Troup, T.N. Tulenko, S.P. Lee, and S.P. Wrenn Detection and characterization of laterally phase separated cholesterol domains in model lipid membranes Colloid Surface B 29 2003 217 231 (Pubitemid 36535495)
42. G.M. Troup, T.N. Tulenko, S.P. Lee, and S.P. Wrenn Estimating the size of laterally phase separated cholesterol domains in model membranes with Forster resonance energy transfer: a simulation study Colloid Surface B 33 2004 57 65 (Pubitemid 38050065)
43. E.J. Wachtel, N. Borochov, and D. Bach The effect of protons or calcium ions on the phase behavior of phosphatidylserine-cholesterol mixtures Biochimica et Biophysica Acta 1066 1991 63 69
44. J. Widomska, M. Raguz, J. Dillon, E.R. Gaillard, and W.K. Subczynski Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studies Biochimica et Biophysica Acta 1768 2007 1454 1465 (Pubitemid 46782711)
45. J. Widomska, M. Raguz, and W.K. Subczynski Oxygen permeability of the lipid bilayer membrane made of calf lens lipids Biochimica et Biophysica Acta 1768 2007 2635 2645 (Pubitemid 47532039)
46. J.J. Yin, and W.K. Subczynski Effects of lutein and cholesterol on alkyl chain bending in lipid bilayers: a pulse electron spin resonance spin labeling study Biophysical Journal 71 1996 832 839 (Pubitemid 26289166)