Male germ cells of the Pacific oyster Crassostrea gigas: Flow cytometry analysis, cell sorting and molecular expression

Aquatic Living Resources

Volumn 24, Issue 3, 2011, Pages 237-245

  Franco, Alban ^a   Kellner, Kristell ^a   Mathieu, Michel ^a   Lelong, Christophe ^a   Goux, Didier ^a   Berthelin, Clothilde Heude ^a  

^a Biology and Innovative Therapeutics for Ovarian Cancers group   (France)

Author keywords

crassostrea gigas; DNA content; gametogenesis; germ line; mitochondrial staining; Mollusca; proliferative cell nuclear antigen; Vasa

Indexed keywords

BIOMARKER; BIVALVE; FLOW CYTOMETRY; GAMETOGENESIS; GENE EXPRESSION; MARINE ECOSYSTEM; MOLECULAR ANALYSIS; REPRODUCTIVE CYCLE;

BIVALVIA; CRASSOSTREA GIGAS; GONIA; MOLLUSCA; OSTREIDAE;

EID: 80155210275     PISSN: 09907440     EISSN: 17652952     Source Type: Journal    
DOI: 10.1051/alr/2011110     Document Type: Article

References (26)

1. Badariotti F., Kypriotou M., Lelong C., Dubos M.-P., Renard E., Galera P., Favrel P., 2006, The phylogenetically conserved molluscan chitinase-like protein 1 (Cg-Clp1), homologue of human HC-gp39, stimulates proliferation and regulates synthesis of extracellular matrix components of mammalian chondrocytes. J. Biol. Chem. 281, 29583-29596. (Pubitemid 44536966)
2. Bellvé A.R., Cavicchia J.C., Millette C.F., O'Brien D.A., Bhatnagar Y.M., Dym, M., 1977, Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization. J. Cell. Biol. 74, 68-85. (Pubitemid 8141486)
3. Berthelin C., Kellner K., Mathieu M., 2000, Histological characterization and glucose incorporation into glycogen of the Pacific oyster Crassostrea gigas storage cells. Mar. Biotechnol. 2, 136-145. (Pubitemid 30416551)
4. Celeghini E. C., de Arruda R. P., de Andrade A. F., Nascimento J., Raphael C. F., 2007, Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes. Reprod. Domest. Anim. 42, 479-488. (Pubitemid 47397307)
5. Com E., Evrard B., Roepstorff P., Aubry F., Pineau C., 2003, New insights into the rat spermatogonial proteome: identification of 156 additional proteins. Mol. Cell. Proteomics 2, 248-261.
6. Fabioux C., Pouvreau S., LeRoux F., Huvet A., 2004, The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas. Biochem. Biophys. Res. Com. 315, 897-904. (Pubitemid 38249388)
7. Fabioux C., Corporeau C., Quillien V., Favrel P., Huvet A., 2009, In vivo RNA interference in oyster-vasa silencing inhibits germ cell development. FEBS J. 276, 2566-2573.
8. Franco A., Heu de Berthelin C., Goux D., Sourdaine P., Mathieu M., 2008, Fine structure of the early stages of spermatogenesis in the Pacific oyster, Crassostrea gigas (Mollusca, Bivalvia). Tissue Cell 40, 251-260.
9. Franco A., Jouaux A., Mathieu M., Sourdaine P., Lelong C., Kellner K., Heu de Berthelin C., 2010, Proliferating cell nuclear antigen in gonad and associated storage tissue of the Pacific oyster Crassostrea gigas: seasonal immunodetection and expression in laser microdissected tissues. Cell Tissue Res. 340, 201-210.
10. Hanquet-Dufour A.C., Kellner K., Heude C., Naimi A., Mathieu M., Poncet J.M., 2006, Cryopreservation of Crassostrea gigas vesicular cells: viability and metabolic activity. Cryobiology 53, 28-36. (Pubitemid 44056050)
11. Heude-Berthelin C., Laisney J., Espinosa J., Martin O., Hernandez G., Mathieu M., Kellner K., 2001, Storage and reproductive strategy in Crassostrea gigas of two different growing area (Normandy and Atlantic shore, France). Invertebr. Reprod. Dev. 40, 79-86. (Pubitemid 34108051)
12. Jouaux A., Heude-Berthelin C., Sourdaine P., Mathieu M., Kellner K., 2010, Gametogenic stages in triploid oysters Crassostrea gigas: Irregular locking of gonial proliferation and subsequent reproductive effort. J. Exp. Mar. Biol. Ecol. 395, 162-170.
13. Kellner K., Heude-Berthelin C., Mathieu M., 2002, Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and an in vitro effect of vertebrate glucagon on glycogen metabolism. Tissue Cell 34, 109-116. (Pubitemid 34965648)
14. Labreuche Y. C., Lambert P., Soudant V., Boulo A., Huvet J., Nicolas L., 2006, Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes Infect. 8, 2715-2724.
15. Le Quéré H., Herpin A., Huvet A., Lelong C., Favrel P., 2009, Structural and functional characterizations of an Activin type II receptor orthologue from the Pacific oyster Crassostrea gigas. Gene 436, 101-107.
16. Lelong C., Badariotti F., Le Quéré H., Rodet F., Dubos M.P., Favrel P., 2007., Cg-TGF-beta, a TGF-beta/activin homologue in the Pacific oyster Crassostrea gigas, is involved in immunity against Gram-negative microbial infection. Dev. Comp. Immunol. 31, 30-38. (Pubitemid 44467972)
17. Loir M., 1994, In vitro approach to the control of spermatogonia proliferation in the trout. Mol. Cell. Endocrinol. 102, 141-150. (Pubitemid 24177487)
18. Loir M., 1999, Spermatogonia of rainbow trout: I. Morphological characterization, mitotic activity, and survival in primary cultures of testicular cells. Mol. Reprod. Dev. 53, 422-433. (Pubitemid 29309933)
19. Loppion G., Crespel A., Martinez A.S.S., Auvray P., Sourdaine P., 2008, Study of the potential spermatogonial stem cell compartment in dogfish testis, Scyliorhinus canicula L. Cell Tissue Res. 332, 533-542.
20. Mann R., 1979, Some biochemical and physiological aspect of growth and gametogenesis in Crassostrea gigas and Ostrea edulis at sustained elevated temperatures. J. Mar. Biol. Assoc. UK 59, 95-100.
21. Misamore M. J., Stein K. K., Lynn J.W., 2006, Sperm incorporation and pronuclear development during fertilization in the freshwater bivalve Dreissena polymorpha. Mol. Reprod. Dev. 73, 1140-1148. (Pubitemid 44148372)
22. Parapanov R., Nusslé S., Hausser J., Vogel P., 2008, Histological description of seminiferous epithelium and cycle length of spermatogenesis in the water shrew Neomys fodiens (Mammalia: Soricidae). Anim. Reprod. Sci. 107, 148-160. (Pubitemid 351698069)
23. Petit J.M., Ratinaud M.H., Cordelli E., Spano M., Julien R., 1995, Mouse testis cell sorting according to DNA and mitochondrial changes during spermatogenesis. Cytometry 19, 304-312.
24. Ruiz C., Martinez D., Mosquera G., Abad M., Sanchez J.L., 1992, Seasonal variations in condition, reproductive activity and biochemical composition of the flat oyster, Ostrea edulis, from San Cibran (Galicia, Spain). Mar. Biol. 112, 67-74. (Pubitemid 23395188)
25. Silandre D., Delalande C., Durand P., Carreau S., 2007, Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells. J. Mol. Endocrinol. 39, 169-181 (Pubitemid 47337875)
26. Suter L., Koch E., Bechter R., Bobadilla M., 1997, Three-parameter flow cytometric analysis of rat spermatogenesis. Cytometry 27, 161-168. (Pubitemid 27101546)