A combined proteomic and immunologic approach for the analysis of schistosoma mansoni cercariae and adult worm protein extracts and the detection of one of the vaccine candidates, Sm28GST, from a venezuelan parasite isolate;Aproximación al análisis proteómico e inmunológico de extractos proteicos de cercaria y verme adulto de Schistosoma mansoni y detección de uno de los candidates a vacuna, Sm28GST, de un aislado venezolano

Investigacion Clinica

Volumn 52, Issue 2, 2011, Pages 121-139

  Losada, Sandra ^a   Sabatier, Laurence ^b   Hammann, Philippe ^b   Guillier, Christelle ^b   Matos, César ^c   Bermúdez, Henry ^a   Lorenzo, María Angelita ^a   Noya, Oscar ^a  

^a UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

^b CNRS   (France)

^c INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS   (Venezuela)

Author keywords

Proteome; Schistosoma; Sm28GST

Indexed keywords

GLUTATHIONE TRANSFERASE; ISOPROTEIN; MICROORGANISM PROTEIN; PARASITE PROTEIN; PARASITE VACCINE; SM28GST PROTEIN; UNCLASSIFIED DRUG; VACCINE; 28 KILODALTON GLUTATHIONE S TRANSFERASE ANTIGEN, SCHISTOSOMA MANSONI; 28-KILODALTON GLUTATHIONE S-TRANSFERASE ANTIGEN, SCHISTOSOMA MANSONI; BACTERIAL ANTIGEN; HELMINTH PROTEIN;

ARTICLE; CERCARIA; EXTRACTION AND EXTRACTS; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOCHEMISTRY; MASS SPECTROMETRY; MICROBIAL METABOLISM; NONHUMAN; PARASITE ISOLATION; PEPTIDE SYNTHESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN SECRETION; PROTEOMICS; RABBIT; SCHISTOSOMA MANSONI; WESTERN BLOTTING; WORM; ANIMAL; GENETICS; IMMUNOLOGY; VENEZUELA;

ANIMALS; ANTIGENS, BACTERIAL; CERCARIA; GLUTATHIONE TRANSFERASE; HELMINTH PROTEINS; PROTEOMICS; SCHISTOSOMA MANSONI; VACCINES; VENEZUELA;

EID: 80155138400     PISSN: 05355133     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Wilson RA, Coulson PS. Immune effector mechanisms against schistosomiasis: looking for a chink in the parasite's armour. Trends Parasitol 2009; 25(9):423-431.
2. Losada S, Chacón N, Colmenares C, Bermúdez H, Lorenzo A, Pointier JP, Theron A, Alarcón de Noya B, Noya O. Schistosoma: cross-reactivity and antigenic community among different species. Exp Parasitol 2005; 111 (3): 182-190.
3. Curwen RS, Ashton PD, Johnston DA, Wilson RA. The Schistosoma mansoni soluble proteome: a comparison across four life-cycle stages. Mol Biochem Parasitol 2004; 138: 57-66.
4. Curwen RS, Ashton PD, Sundaralingam S, Wilson RA. Identification of novel proteases and immunomodulators in the secretions of schistosome cercariae that facilitate host entry. Mol Cell Proteomics 2006; 5: 835-844.
5. Knudsen GM, Medzihradszky KF, Lim KC, Hansell E, McKerrow JH. Proteomic analysis of Schistosoma mansoni cercarial secretions. Mol Cell Proteomics 2005; 4: 1862-1875.
6. Braschi S, Borges WC, Wilson RA. Proteomic analysis of the schistosome tegument and its surface membranes. Mem Inst Oswaldo Cruz 2006; 101 (Suppl 1): 205-212.
7. Hansell E, Braschi S, Medzihradszky KF, Sajid M, Debnath M, Ingram J, Lim KC, McKerrow JH. Proteomic analysis of skin invasion by blood fluke larvae. PLoS Negl Trop Dis 2008; 162:e262.
8. El Ridi R, Tallima H. Schistosoma mansoni ex vivo lung-stage larvae excretory-secretory antigens as vaccine candidates against schistosomiasis. Vaccine 2009; 27(5): 666-673.
9. Delcroix M, Medzihradsky K, Caffrey CR, Fetter RD, McKerrow JH. Proteomic analysis of adult S. mansoni gut contents. Mol Biochem Parasitol 2007; 154: 95-97.
10. van Balkom BW, van Gestel RA, Brouwers JF, Krijgsveld J, Tielens AG, Heck AJ, van Hellemond JJ. Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome. J Proteome Res 2005; 4: 958-966.
11. Wu X, Sabat G, Brown JF, Zhanga M, Taft A, Peterson N, Harms A, Yoshino T. Proteomic analysis of Schistosoma mansoni proteins released during in vitro miracidium-to-sporocyst transformation Mol Biochem Parasitol 2009; 164: 32-44.
12. DeMarco R, Verjovski-Almeida S. Schistosomes-proteomics studies for potential novel vaccines and drug targets. Drug Discov Today 2009; 14(9-10): 472-478.
13. Balloul JM, Sondermeyer P, Dreyer D, Capron M, Grzych JM, Pierce RJ, Carvallo D, Lecocq JP, Capron A. Molecular cloning of a protective antigen of schistosomes. Nature 1987; 326: 149-153.
14. Capron A, Riveau G, Capron M, Trottein F. Schistosomes: the road from host-parasite interactions to vaccines in clinical trials. Trends Parasitol 2005; 21: 143-149.
15. Miyasato PA, Ramos CRR, Abreu PAE, Dias WO, Nascimento C, Ho PL, Kawaro T. Expression of schistosomal cathepsin L1 in Escherichia coli and evaluation of its protective capacity in an animal challenge. J Venom Anim Toxins incl Trop Dis 2009; 15(2): 289-304.
16. Haag KL, Gottstein B, Müller N, Schnorr A, Ayala FJ. Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes? Parasitology 2006; 133(Pt 4): 411-419.
17. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
18. Chahed K, Kabbage M, Hamrita B, Guillier CL, Trimeche M, Remadi S, Ehret-Sabatier L, Chouchane L. Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis. Clin Chim Acta 2008; 388(1-2): 106-114.
19. Morrissey JH. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 1981; 117(2): 307-310.
20. Rabilloud T, Strub J M, Luche S, Van Dorsselaer A, Lunardi J. A comparison between Sypro Ruby and ruthenium II Tris (bathophenanthroline disulfonate) as fluorescent strains for protein detection in gels. Proteomics 2001; 5: 699-704.
21. Merrifield RB. Solid-phase peptide synthesis. The synthesis of a tetrapeptide. J Am Chem Soc 1963; 85: 2149-2154.
22. Houghten RA, DeGraw ST, Bray MK, Hoffmann S, Frizzell ND. Simultaneous multiple peptide synthesis: The rapid preparation of large number of discrete peptides for biological, immunological and methodological studies. BioTechniques 1986; 4: 522-528.
23. Patarroyo ME, Romero P, Torres ML, Clavijo P, Moreno A, Martínez A, Rodriguez R, Guzman F, Cabezas E. Induction of protective immunity against experimental infection with malaria using synthetic peptides. Nature 1987; 328: 629-632.
24. Noya O, Alarcón de Noya B, Ballen DE, Bermúdez H, Bout D, Hoebeke J. Immunogenicity of synthetic peptides from the Sm31 antigen (cathepsin B) of the Schistosoma mansoni adult worms. Parasite Immunol 2001; 23(11): 567-573.
25. Wolowczuk I, Auriault C, Bossus M, Boulanger D, Gras-Masse H, Mazingue C, Pierce RJ, Grezel D, Reid GD, Tartar A. Antigenicity and immunogenicity of a multiple peptidic construction of the Schistosoma mansoni Sm-28 GST antigen in rat, mouse, and monkey. 1. Partial protection of Fischer rat after active immunization. J Immunol 1991; 146: 1987-1995.
26. Auriault C, Gras-Masse H, Pierce RJ, Butterworth AE, Wolowczuk I, Capron M, Ouma JH, Balloul JM, Khalife J, Neyrinck JL, Tartar A, Koech D, Capron A. Antibody response of Schistosoma mansoni-infected human subjects to the recombinant P28 glutathione-S-transferase and to synthetic peptides. J Clin Microbiol 1990; 28: 1918-1924.
27. Xu CB, Verwaerde C, Gras-Masse H, Fontaine J, Bossus M, Trottein F, Wolowczuk I, Tartar A, Capron A. Schistosoma mansoni 28-kDa glutathione S-transferase and immunity against parasite fecundity and egg viability. Role of the amino- and carboxyl-terminal domains. J Immunol 1993; 150: 940-949.
28. Pancré V, Wolowczuk I, Bossus M, Gras-Masse H, Guerret S, Delanoye A, Capron A, Auriault C. Evaluation of the effect of Sm28GST-derived peptides in murine hepatosplenic schistosomiasis: interest of the lipopeptidic form of the C-ter-minal peptide. Mol Immunol 1994; 31: 1247-1256.
29. Wilson RA, Ashton PD, Braschi S, Dillon GP, Berriman M, Ivens A. Oming in on schistosomes: prospects and limitations for post-genomics. Trends Parasitol 2007; 23(1): 14-20.
30. Verjovski-Almeida S, DeMarco R. Current developments on Schistosoma proteomics Acta Trop 2008; 108(2-3): 183-185.
31. Cass CL, Johnson JR, Califf LL, Xu T, Hernandez HJ, Stadecker MJ., Yates JR. 3rd, Williams DL. Proteomic analysis of Schistosoma mansoni egg secretions. Mol Biochem Parasitol. 2007; 155(2): 84-93.
32. Roger E, Grunau C, Pierce RJ, Hirai H, Gourbal B, Galinier R, Emans R, Cesari IM, Cosseau C, Mitta G. Controlled chaos of polymorphic mucins in a metazoan parasite (Schistosoma mansoni) interacting with its invertebrate host (Biomphalaria glabrata). PLoS Negl Trop Dis 2008; 2(11): e330.
33. Wolowczuk I, Auriault C, Gras-Masse H, Vendeville C, Balloul JM, Tartar A, Capron A. Protective immunity in mice vaccinated with the Schistosoma mansoni P-28-1 antigen. J Immunol 1989; 142(4): 1342-1350.
34. Boulanger D, Reid GD, Sturrock RF, Wolowczuk I, Balloul JM, Grezel D, Pierce RJ, Otieno MF, Guerret S, Grimaud JA, Butterworth AE, Capron A. Immunization of mice and baboons with the recombinant Sm28GST affects both worm viability and fecundity after experimental infection with Schistosoma mansoni. Parasite Immunol 1991; 13: 473-490.
35. Pérez-Sánchez R, Ramajo-Hernández A, Ramajo-Marín V, Oleaga A. Proteomic analysis of the tegument and excretory-secretory products of adult Schistosoma bovis worms. Proteomics 2006; (Suppl 1): S226-s236.
36. Pérez-Sánchez R, Valero ML, Ramajo-Hernández A, Siles-Lucas M, Ramajo-Martín V, Oleaga A. A proteomic approach to the identification of tegumental proteins of male and female Schistosoma bovis worms. Mol Biochem Parasitol 2008; 161(2): 112-123.
37. Salter JP, Choe Y, Albrecht H, Franklin C, Lim KC, Craik CS, McKerrow JH. Cercarial elastase is encoded by a functionally conserved gene family across multiple species of schistosomes. J Biol Chem 2002, 277(27): 24618-24624.
38. Planchart S, Incani RN, Cesari IM. Preliminary characterization of an adult worm "vomit" preparation of Schistosoma mansoni and its potential use as antigen for diagnosis. Parasitol Res 2007; 101(2):301-309.
39. Cohen C, Reinhardt B, Castellani L, Norton P, Stirewalt M. Schistosome surface spines are "crystals" of actin. J Cell Biol 1982; 95(3): 987-988.
40. Braschi S, Wilson RA. Proteins exposed at the adult schistosome surface revealed by biotinylation. Mol Cell Proteomics 2006; 5(2):347-356.
41. Tallima H, El Ridi R. Re: is actin the praziquantel receptor? Int J Antimicrob Agents 2007; 30(6):566-567.
42. McNair AT, Dissous C, Duvaux-Miret O, Capron A. Cloning and characterisation of the gene encoding the 28-kDa Glutathione S-transferase of Schistosoma mansoni. Gene 1993; 124(2):245-249.
43. Girardini J, Amirante A, Zemzoumi K, Serra E. Characterization of an omegaclass glutathione S-transferase from Schistosoma mansoni with glutaredoxinlike dehydroascorbate reductase and thiol transferase activities Eur J Biochem 2002; 269: 5512-5521.