Immunogenicity of synthetic peptides from the Sm31 antigen (cathepsin B) of the Schistosoma mansoni adult worms

Parasite Immunology

Volumn 23, Issue 11, 2001, Pages 567-573

  Noya, Oscar ^a,c   Alarcón De Noya, Belkisyolé ^a   Ballen, Diana E ^a   Bermúdez, Henry ^a   Bout, Daniel ^b   Hoebeke, Johan ^b  

^a UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

^b UNIVERSITÉ DE TOURS   (France)

^c UNIVERSIDAD CENTRAL DE VENEZUELA   (Venezuela)

Author keywords

Cathepsin B; Schistosoma mansoni; Sm31; Synthetic peptides

Indexed keywords

CATHEPSIN B; CYSTEINYLGLYCYLASPARTYLHISTIDYLASPARAGINYLASPARTYLTRYPTOPHYLASPARAGINYLVALY LGLUTAMYLISOLEUCYLPROLYLSERYLASPARAGINYLPHENYLALANYLASPARTYLSERYLARGINYLLYSYLLYS YLTRYPTOPHYLPROLYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLGLUTAMINYLARGINYLLYSYLTYROSYLLYSYLTHREONYLPROLYLTYROSYLTHREO NYLGLUTAMINYLASPARTYLLYSYLHISTIDYLARGINYLGLYCYLLYSYLSERYLSERYLTYROSYLASPARAGINYL GLYCYLCYSTEINE; CYSTEINYLGLYCYLGLUTAMYLLEUCYLSERYLALANYLVALYLASPARTYLLEUCYLLEUCYLTHREONYLCY STEINYLCYSTEINYLGLUTAMYLSERYLCYSTEINYLGLYCYLLEUCYLGLYCYLCYSTEINYLGLUTAMYLGLYCYLG LYCYLCYSTEINE; CYSTEINYLGLYCYLGLYCYLALANYLVALYLGLUTAMYLALANYLMETHIONYLSERYLASPARTYLARGINYL SERYLCYSTEINYLISOLEUCYLGLUTAMINYLSERYLGLYCYLGLYCYLLYSYLGLUTAMINYLASPARAGINYLVALY LGLYCYLCYSTEINE; CYSTEINYLGLYCYLGLYCYLCYSTEINYLLYSYLSERYLISOLEUCYLALANYLTHREONYLISOLEUCYLARG INYLASPARTYLGLUTAMINYLSERYLARGINYLCYSTEINYLGLYCYLSERYLCYSTEINYLTRYPTOPHYLSERYLPH ENYLALANYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLGLYCYLGLUTAMYLALANYLLEUCYLGLYCYLGLYCYLHISTIDYLALANYLISOLEUCY LARGINYLISOLEUCYLISOLEUCYLGLYCYLTRYPTOPHYLGLYCYLVALYLGLUTAMYLASPARAGINYLLYSYLTHR EONYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLGLYCYLISOLEUCYLLEUCYLGLYCYLPROLYLALANYLTRYPTOPHYLASPARTYLTYR OSYLTRYPTOPHYLVALYLLYSYLGLUTAMYLGLYCYLISOLEUCYLVALYLTHREONYLALANYLSERYLSERYLGLYC YLCYSTEINE; CYSTEINYLGLYCYLGLYCYLLYSYLTYROSYLPROLYLPROLYLCYSTEINYLGLYCYLSERYLLYSYLISOLE UCYLTYROSYLASPARAGINYLTHREONYLPROLYLARGINYLCYSTEINYLLYSYLGLUTAMINYLTHREONYLCYSTE INYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLISOLEUCYLGLUTAMINYLMETHIONYLGLYCYLALANYLARGINYLARGINYLGLUTAM YLGLUTAMYLPROLYLASPARTYLLEUCYLARGINYLARGINYLLYSYLARGINYLARGINYLPROLYLTHREONYLVAL YLGLYCYLCYSTEINE; CYSTEINYLGLYCYLISOLEUCYLVALYLARGINYLGLYCYLARGINYLASPARTYLGLUTAMYLCYSTEINYLS ERYLISOLEUCYLGLUTAMYLSERYLGLUTAMYLVALYLISOLEUCYLALANYLGLYCYLARGINYLISOLEUCYLASPA RAGINYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLLYSYLGLUTAMYLASPARAGINYLHISTIDYLTHREONYLGLYCYLCYSTEINYLGLUTA MYLPROLYLTYROSYLPROLYLPHENYLALANYLPROLYLLYSYLCYSTEINYLGLUTAMYLHISTIDYLHISTIDYLTH REONYLLYSYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLMETHIONYLLEUCYLTHREONYLSERYLISOLEUCYLLEUCYLCYSTEINYLISOLEUCY LALANYLSERYLLEUCYLISOLEUCYLTHREONYLPHENYLALANYLLEUCYLGLUTAMYLALANYLHISTIDYLISOLE UCYLSERYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLPROLYLASPARAGINYLALANYLGLYCYLTRYPTOPHYLARGINYLALANYLGLUTAMYL LYSYLSERYLASPARAGINYLARGINYLPHENYLALANYLHISTIDYLSERYLLEUCYLASPARTYLASPARTYLALANY LARGINYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLPROLYLTYROSYLTRYPTOPHYLLEUCYLISOLEUCYLALANYLASPARAGINYLSERYL TRYPTOPHYLASPARAGINYLGLUTAMYLASPARTYLTRYPTOPHYLGLYCYLGLUTAMYLASPARAGINYLGLYCYLTY ROSYLPHENYLALANYLARGINYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLSERYLPHENYLALANYLTHREONYLVALYLTYROSYLGLUTAMYLASPARTYLPHENYLA LANYLLEUCYLASPARAGINYLTYROSYLLYSYLSERYLGLYCYLISOLEUCYLTYROSYLLYSYLHISTIDYLISOLEU CYLTHREONYLGLYCYLCYSTEINE; CYSTEINYLGLYCYLVALYLLYSYLASPARAGINYLASPARTYLGLUTAMYLLYSYLALANYLISOLEUCYLGLU TAMINYLLYSYLGLUTAMYLISOLEUCYLMETHIONYLLYSYLTYROSYLGLYCYLPROLYLVALYLGLUTAMYLALANY LGLYCYLCYSTEINE; CYSTEINYLGLYCYLVALYLLYSYLASPARAGINYLGLUTAMYLLYSYLPHENYLALANYLGLUTAMYLPROLYL LEUCYLSERYLASPARTYLASPARTYLISOLEUCYLISOLEUCYLSERYLTYROSYLISOLEUCYLASPARAGINYLGLU TAMYLHISTIDYLGLYCYLCYSTEINE; PARASITE ANTIGEN; PEPTIDE ANTIBODY; SM31 ANTIGEN; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIGEN RECOGNITION; ANTIGENICITY; ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; IMMUNIZATION; IMMUNOCYTOCHEMISTRY; IMMUNOGENICITY; INTESTINE; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; RABBIT; SCHISTOSOMA MANSONI; SCHISTOSOMIASIS; SEQUENCE HOMOLOGY; SERODIAGNOSIS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; BLOTTING, WESTERN; CYSTEINE ENDOPEPTIDASES; HELMINTH PROTEINS; IMMUNOHISTOCHEMISTRY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; SCHISTOSOMA MANSONI;

ORYCTOLAGUS CUNICULUS; SCHISTOSOMA; SCHISTOSOMA MANSONI; TREMATODA;

EID: 0035723856     PISSN: 01419838     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3024.2001.00414.x     Document Type: Article

References (26)

1. New approaches for the control and erradication of schistosomiasis in Venezuela
2. Possible use of Schistosoma mansoni enzymes as antigens for immunodiagnosis
3. Schistosoma mansoni diagnostic antigens (Sm 31/32): A sero epidemiological study in the Sudan
4. Evaluation of alkaline phosphatase immunoassay and comparison with other diagnostic methods in areas of low transmission of schistosomiasis
5. Schistosoma mansoni: Immunoblot analysis of adult worm proteins
6. Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: Diagnostic potential of an adult schistosome polypeptide
7. Diagnostic Mr 31 000 Schistosoma mansoni proteins: Requirement of infection, but not immunization and use of the 'miniblot' technique for the production of monoclonal antibodies
8. Humoral immune response of children with chronic schistosomiasis. Isotype recognition of adult worm antigens
9. Sera of Schistosoma japonicum infected patients cross-react with diagnostic 31/32 proteins of Schistosoma mansoni
10. Diagnostic 31/32000 Schistosoma mansoni proteins (Sm31/32) reaction with sera from Sudanese patients infected with Schistosoma mansoni or Schistosoma haematobium
11. Diagnostic Mr 31/32000 of Schistosoma mansoni (Sm31/32) and Schistosoma haematobium (Sh 31/32): Stability and reaction conditions for a prospective field test
12. Primary structures of Sm 31/32 diagnostic proteins of Schistosoma mansoni and their identification as proteases
13. Immunological analysis of cloned Schistosoma mansoni antigens Sm31 and Sm32 with sera of schistosomiasis patients
14. Solid phase peptide synthesis: The synthesis of a tetrapeptide
15. Simultaneous multiple peptide synthesis: The rapid preparation of large numbers of peptides for biological, immunological, and methodological studies
16. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worm
17. Protein measurements with the folin reagent
18. Cleavage of structural proteins during the assembly of the head of bacteriophague T4
19. Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: Procedure and some applications
20. The multiple antigen blot assay (MABA): A simple immunoenzymatic technique for simultaneous screening of multiple antigens
21. Prediction of protein antigen determinants from amino acid sequences
22. Schistosoma mansoni: Localization of antigenic regions on the 31 kilodalton diagnostic protein
23. Expression of diagnostic 31/32 kilodalton proteins of Schistosoma mansoni as fusions with bacteriophage MS2 polymerase
24. Properties of different artificial antigen immunologically related to tobacco mosaic virus