Live cell imaging of F-actin dynamics via Fluorescent Speckle Microscopy (FSM)

Journal of Visualized Experiments

Volumn , Issue 30, 2009, Pages

  Lim, James ^a   Danuser, Gaudenz ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Actin; Cellular Biology; Cytoskeleton; Fluorescence; FSM; Issue 30; Microinjection; Microscopy; qFSM; Speckle

Indexed keywords

EID: 80055119116     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1325     Document Type: Article

References (5)

1. Waterman-Storer C: Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells. Curr Protoc Cell Biol, Chapter 4:Unit 4 10 (2002).
2. Ponti A, Machacek M, Gupton SL, Waterman-Storer CM, Danuser G: Two distinct actin networks drive the protrusion of migrating cells. Science, 305:1782-1786 (2004).
3. Zhang XF, Schaefer AW, Burnette DT, Schoonderwoert VT, Forscher P: Rho-dependent contractile responses in the neuronal growth cone are independent of classical peripheral retrograde actin flow. Neuron, 40:931-944 (2003).
4. Salmon WC, Adams MC, Waterman-Storer CM: Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells. J Cell Biol, 158:31-37 (2002).
5. Danuser G, Waterman-Storer CM: Quantitative fluorescent speckle microscopy of cytoskeleton dynamics. Annu Rev Biophys Biomol Struct, 35:361-387 (2006).