A Conserved peptide in West Nile virus NS4A protein contributes to proteolytic processing and is essential for replication

Journal of Virology

Volumn 85, Issue 21, 2011, Pages 11274-11282

  Ambrose, R L ^a   Mackenzie, J M ^a  

^a LA TROBE UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ASPARTIC ACID; DOUBLE STRANDED RNA; NONSTRUCTURAL PROTEIN 4A; PEPTIDE; PROLINE; VIRUS PROTEIN; VIRUS RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CLONE; COMPLEX FORMATION; CONTROLLED STUDY; EMBRYO; GENE DELETION; HUMAN; HUMAN CELL; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROCESSING; PROTEIN SYNTHESIS; REPLICON; RNA REPLICATION; RNA SYNTHESIS; VIRION; VIROGENESIS; VIRUS ATTENUATION; VIRUS MUTANT; VIRUS REPLICATION; WEST NILE FLAVIVIRUS;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; CONSERVED SEQUENCE; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; POINT MUTATION; VIRAL NONSTRUCTURAL PROTEINS; VIRULENCE; VIRULENCE FACTORS; VIRUS REPLICATION; WEST NILE VIRUS;

KUNJIN VIRUS; WEST NILE VIRUS;

EID: 80055114110     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.05864-11     Document Type: Article

References (22)

1. Ambrose, R. L., and J. M. Mackenzie. 2011. West Nile Virus differentially modulates the unfolded protein response to facilitate replication and immune evasion. J. Virol. 85:2723-2732.
2. Coia, G., M. D. Parker, G. Speight, M. E. Byrne, and E. G. Westaway. 1988. Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. J. Gen. Virol. 69:1-21.
3. Gillespie, L. K., A. Hoenen, G. Morgan, and J. M. Mackenzie. 2010. The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex. J. Virol. 84:10438-10447.
4. Khromykh, A. A., M. T. Kenney, and E. G. Westaway. 1998. trans-Complementation of flavivirus RNA polymerase gene NS5 by using Kunjin virus replicon-expressing BHK cells. J. Virol. 72:7270-7279.
5. Khromykh, A. A., P. L. Sedlak, K. J. Guyatt, R. A. Hall, and E. G. Westaway. 1999. Efficient trans-complementation of the flavivirus kunjin NS5 protein but not of the NS1 protein requires its coexpression with other components of the viral replicase. J. Virol. 73:10272-10280.
6. Lin, C., S. M. Amberg, T. J. Chambers, and C. M. Rice. 1993. Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site. J. Virol. 67:2327-2335.
7. Lindenbach, B. D., and C. M. Rice. 1999. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J. Virol. 73:4611-4621.
8. Lindenbach, B. D., and C. M. Rice. 1997. trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication. J. Virol. 71:9608-9617.
9. Liu, W. J., et al. 2005. Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins. J. Virol. 79:1934-1942.
10. Lobigs, M., and E. Lee. 2004. Inefficient signalase cleavage promotes efficient nucleocapsid incorporation into budding flavivirus membranes. J. Virol. 78: 178-186.
11. Mackenzie, J. 2005. Wrapping things up about virus RNA replication. Traffic 6:967-977.
12. Mackenzie, J. M., A. A. Khromykh, M. K. Jones, and E. G. Westaway. 1998. Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A. Virology 245:203-215.
13. Mertens, E., et al. 2010. Viral determinants in the NS3 helicase and 2K peptide that promote West Nile virus resistance to antiviral action of 2',5'-oligoadenylate synthetase 1b. Virology 399:176-185.
14. Miller, S., S. Kastner, J. Krijnse-Locker, S. Buhler, and R. Bartenschlager. 2007. Non-structural protein 4A of Dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner. J. Biol. Chem. 282:8873-8882.
15. Miller, S., S. Sparacio, and R. Bartenschlager. 2006. Subcellular localization and membrane topology of the Dengue virus type 2 non-structural protein 4B. J. Biol. Chem. 281:8854-8863.
16. Roosendaal, J., E. G. Westaway, A. Khromykh, and J. M. Mackenzie. 2006. Regulated cleavages at the West Nile virus NS4A-2K-NS4B junctions play a major role in rearranging cytoplasmic membranes and Golgi trafficking of the NS4A protein. J. Virol. 80:4623-4632.
17. Varnavski, A. N., P. R. Young, and A. A. Khromykh. 2000. Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors. J. Virol. 74:4394-4403.
18. von Heijne, G. 1991. Proline kinks in transmembrane -helices. J. Mol. Biol. 218:499-503.
19. Westaway, E. G., J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh. 1997. Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures. J. Virol. 71:6650-6661.
20. Westaway, E. G., J. M. Mackenzie, and A. A. Khromykh. 2003. Kunjin RNA replication and applications of Kunjin replicons. Adv. Virus Res. 59:99-140.
21. Westaway, E. G., J. M. Mackenzie, and A. A. Khromykh. 2002. Replication and gene function in Kunjin virus. Curr. Top. Microbiol. Immunol. 267:323-351.
22. Williams, K. A., and C. M. Deber. 1991. Proline residues in transmembrane helices-structural or dynamic role. Biochemistry 30:8919-8923.