Neutrophil extracellular traps: How to generate and visualize them

Journal of Visualized Experiments

Volumn , Issue 36, 2010, Pages

  Brinkmann, Volker ^a   Laube, Britta ^a   Abed, Ulrike Abu ^a   Goosmann, Christian ^a   Zychlinsky, Arturo ^a  

^a MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055113935     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1724     Document Type: Article

References (7)

1. Brinkmann, V., et al. Neutrophil extracellular traps kill bacteria. Science 303, 1532-1535,(2004).
2. Urban, C. F., Reichard, U., Brinkmann, V. & Zychlinsky, A. Neutrophil extracellular traps capture and kill Candida albicans yeast and hyphalforms. Cell Microbiol 8, 668-676 (2006).
3. Guimaraes-Costa, A. B., Nascimento, M.T.C., Froment, G.S., Soares, R.P.P., Morgado, F.N., Conceicao-Silva, F., and Saraiva E.M.Leishmania amazonensis promastigotes induce and are killed by neutrophil extracellular traps. PNAS 106:6748-6753, (2009).
4. Buchanan, J.T., Simpson, A.J., Aziz, R.K., Liu, G.Y., Kristian, S.A., Kotb, M., Feramisco, J., and Nizet, V. DNase expression allows thepathogen group A Streptococcus to escape killing in neutrophil extracellular traps. Curr Biol. 16:396-400 (2006).
5. Fuchs, T. A, et al. Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 176, 231-241 (2007).
6. Brinkmann, V., & Zychlinsky, A. Beneficial suicide: why neutrophils die to make NETs. Nat Rev Microbiol 5, 577-582 (2007).
7. Aga, E., Katschinski, D.M., van Zandbergen, G., Laufs, H., Hansen, B., Muller, K., Solbach, W., and Laskay, T. Inhibition of the spontaneousapoptosis of neutrophil granulocytes by the intracellular parasite Leishmania major. J Immunol. 169:898-905 (2002).