Large insert environmental genomic library production

Journal of Visualized Experiments

Volumn , Issue 31, 2009, Pages

  Taupp, Marcus ^a   Lee, Sangwon ^a   Hawley, Alyse ^a   Yang, Jinshu ^a   Hallam, Steven J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055111975     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1387     Document Type: Article

References (7)

1. Zaikova, E., Hawley, A., Walsh, D.A. & Hallam, S.J. Seawater sampling and collection. JoVE. 28, (2009).
2. Walsh, D.A., Zaikova, E. & Hallam, S.J. Large volume (20L+) filtration of coastal seawater samples. JoVE. 28, (2009).
3. Wright JJ, Lee S, Zaikova E, Walsh DA, Hallam SJ (2009). DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation. JoVE. 31. http://www.jove.com/index/details.stp?id=1352, doi: 10.3791/1352
4. Stein, J.L., Marsh, T.L., Wu, K.Y., Shizuya, H. & DeLong, E.F. Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J. Bacteriol. 178, 591-599 (1996).
5. Béjà, O., Suzuki, M.T., Koonin, E.V., Aravind, L., Hadd, A., Nguyen L.P., Villacorta, R., Amjadi, M., Garrigues, C., Jovanovich, S.B., Feldman, R.A. & DeLong, E.F. Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage. Environ. Microbiol. 2, 516-529 (2000).
6. DeLong, E.F., Preston, C.M., Mincer, T., Rich, V., Hallam, S.J., Frigaard, N.-U., Martinez, A., Sullivan, M.B., Edwards, R., Brito, B.R., Chisholm, S.W. & Karl, D.M. Community genomics among stratified microbial assemblages in the Ocean's interior. Science 311, 496-503 (2006).
7. Hallam, S.J., Putnam, N., Preston, C.M., Detter, J.C., Rokhsar, D., Richardson, P.M. & DeLong, E.F. Reverse methanogenesis: testing the hypothesis with environmental genomics. Science 305, 1457-1462 (2004).