Identification of growth inhibition phenotypes induced by expression of bacterial type III effectors in yeast

Journal of Visualized Experiments

Volumn , Issue 37, 2010, Pages

  Salomon, Dor ^a   Sessa, Guido ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

Gram negative bacteria; Issue 37; Microbiology; Saccharomyces cerevisiae; Type III effector proteins; Type III secretion system; Yeast expression system

Indexed keywords

EID: 80055106290     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1865     Document Type: Article

References (6)

1. Siggers, K. A. & Lesser, C. F. The yeast Saccharomyces cerevisiae: a versatile model system for the identification and characterization of bacterial virulence proteins. Cell Host Microbe 4, 8-15 (2008).
2. Parsons, A. B. et al. Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways. Nat. Biotechnol. 22, 62-69 (2004).
3. Munkvold, K. R., Martin, M. E., Bronstein, P. A. & Collmer, A. A survey of the Pseudomonas syringae pv. tomato DC3000 type III secretion system effector repertoire reveals several effectors that are deleterious when expressed in Saccharomyces cerevisiae. Mol. Plant-Microbe Interact. 21, 490-502 (2008).
4. Curak, J., Rohde, J. & Stagljar, I. Yeast as a tool to study bacterial effectors. Curr. Opin. Microbiol. 12, 18-23 (2009).
5. Slagowski, N. L., Kramer, R. W., Morrison, M. F., LaBaer, J. & Lesser, C. F. A functional genomic yeast screen to identify pathogenic bacterial proteins. PLoS Pathog. 4, e9 (2008).
6. Huang, J., Lesser, C. F. & Lory, S. The essential role of the CopN protein in Chlamydia pneumoniae intracellular growth. Nature 456, 112-115 (2008).