Rapid PCR thermocycling using microscale thermal convection

Journal of Visualized Experiments

Volumn , Issue 49, 2011, Pages

  Muddu, Radha ^a   Hassan, Yassin A ^a   Ugaz, Victor M ^a  

^a Texas AandM University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055105196     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2366     Document Type: Article

References (12)

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3. Braun, D., Goddard, N. L. & Libchaber, A. Exponential DNA replication by laminar convection. Physical Review Letters 91, 158103 (2003).
4. Chen, Z., Qian, S., Abrams, W. R., Malamud, D. & Bau, H. Thermosiphon-based PCR reactor: experiment and modeling. Analytical Chemistry 76, 3707-3715 (2004).
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8. Yao, D. J., Chen, J. R. & Ju, W. T. Micro-Rayleigh-Benard convection polymerase chain reaction system. Journal of Micro-Nanolithography MEMS and MOEMS 6, 043007 (2007).
9. Agrawal, N., Hassan, Y. A. & Ugaz, V. M. A Pocket-sized Convective PCR Thermocycler. Angewandte Chemie International Edition 46, 4316-4319 (2007).
10. Yariv, E., Ben-Dov, G. & Dorfman, K. D. Polymerase chain reaction in natural convection systems: A convection-diffusion-reaction model. Europhysics Letters 71, 1008-1014 (2005).
11. Allen, J. W., Kenward, M. & Dorfman, K. D. Coupled flow and reaction during natural convection PCR. Microfluidics and Nanofluidics 6, 121-130 (2009).
12. Muddu, R., Hassan, Y. A. & Ugaz, V. M. Accelerated Biochemistry by Chaotic Stirring. In review (2010).