Analysis of DNA double-strand break (DSB) repair in mammalian cells

Journal of Visualized Experiments

Volumn , Issue 43, 2010, Pages

  Seluanov, Andrei ^a   Mao, Zhiyong ^a   Gorbunova, Vera ^a  

^a UNIVERSITY OF ROCHESTER   (United States)

Author keywords

Cellular Biology; DNA repair; HR; Issue 43; Mammalian cells; NHEJ

Indexed keywords

EID: 80055104335     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2002     Document Type: Article

References (6)

1. Mao, Z., Seluanov, A., Jiang, Y. & Gorbunova, V. TRF2 is required for repair of nontelomeric DNA double-strand breaks by homologous recombination. Proc Natl Acad Sci U S A 104, 13068-73 (2007).
2. Mao, Z., Bozzella, M., Seluanov, A. & Gorbunova, V. Comparison of nonhomologous end joining and homologous recombination in human cells. DNA Repair (Amst) 7, 1765-71 (2008).
3. Rouet, P., Smih, F. & Jasin, M. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Proc Natl Acad Sci U S A 91, 6064-8 (1994).
4. Mao, Z., Jiang, Y., Xiang, L., Seluanov, A. & Gorbunova, V. DNA repair by homologous recombination, but not by nonhomologous end joining, is elevated in breast cancer cells. Neoplasia 11, 683-91 (2009).
5. Seluanov, A., Mittelman, D., Pereira-Smith, O.M., Wilson, J.H. & Gorbunova, V. DNA end joining becomes less efficient and more error-prone during cellular senescence. Proc Natl Acad Sci U S A 101, 7624-9 (2004).
6. Mao, Z., Bozzella, M., Seluanov, A. & Gorbunova, V. DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells. Cell Cycle 7, 2902-6 (2008).