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Volumn 9, Issue AUG, 2011, Pages 329-339

Optimized routine flow cytometric enumeration of heterotrophic flagellates using SYBR Green I

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); MASTIGOPHORA (FLAGELLATES);

EID: 80055088853     PISSN: None     EISSN: 15415856     Source Type: Journal    
DOI: 10.4319/lom.2011.9.329     Document Type: Article
Times cited : (86)

References (49)
  • 1
    • 0022981460 scopus 로고
    • Fixation, counting and manipulation of heterotrophic nanoflagellates
    • Bloem, J., M. J. Bar-Gilissen, and T. E. Cappenberg. 1986. Fixation, counting and manipulation of heterotrophic nanoflagellates. Appl. Environ. Microbiol. 52:1266-1272.
    • (1986) Appl. Environ. Microbiol. , vol.52 , pp. 1266-1272
    • Bloem, J.1    Bar-Gilissen, M.J.2    Cappenberg, T.E.3
  • 2
    • 34447327017 scopus 로고    scopus 로고
    • A comparative study of the cytometric characteristics of high and low nucleic-acid bacterioplankton cells from different aquatic ecosystems
    • Bouvier, T., P. A. del Giorgio, and J. M. Gasol. 2007. A comparative study of the cytometric characteristics of high and low nucleic-acid bacterioplankton cells from different aquatic ecosystems. Environ. Microbiol. 9:2050-2066
    • (2007) Environ. Microbiol. , vol.9 , pp. 2050-2066
    • Bouvier, T.1    del Giorgio, P.A.2    Gasol, J.M.3
  • 3
    • 1642386112 scopus 로고    scopus 로고
    • Optimization of procedures for counting viruses by flow cytometry
    • Brussaard, C. P. D. 2004. Optimization of procedures for counting viruses by flow cytometry. Appl. Environ. Microbiol. 70:1506-1513
    • (2004) Appl. Environ. Microbiol. , vol.70 , pp. 1506-1513
    • Brussaard, C.P.D.1
  • 4
    • 0002047169 scopus 로고
    • Protozoan nutrient regeneration
    • Oxford Univ. Press, G. M. Capriulo [eds.]
    • Caron, D. A., and J. C. Goldman. 1990. Protozoan nutrient regeneration, p. 283-306. In G. M. Capriulo [eds.], Ecology of marine protozoa. Oxford Univ. Press.
    • (1990) Ecology of marine protozoa , pp. 283-306
    • Caron, D.A.1    Goldman, J.C.2
  • 5
    • 0033035785 scopus 로고    scopus 로고
    • Picoplankton and nanoplankton and their trophic coupling in surface waters of the Sargasso Sea south of Bermuda
    • Caron, D. A., E. R. Peele, E. L. Lim, and M. R. Dennett. 1999. Picoplankton and nanoplankton and their trophic coupling in surface waters of the Sargasso Sea south of Bermuda. Limnol. Oceanogr. 44:259-272
    • (1999) Limnol. Oceanogr. , vol.44 , pp. 259-272
    • Caron, D.A.1    Peele, E.R.2    Lim, E.L.3    Dennett, M.R.4
  • 6
    • 0035194320 scopus 로고    scopus 로고
    • Nanoflagellate predation on auto- and heterotrophic picoplankton in the oligotrophic Mediterranean Sea
    • Christaki, U., A. Giannakourou, F. Van Wambeke, and G. Gregori. 2001. Nanoflagellate predation on auto- and heterotrophic picoplankton in the oligotrophic Mediterranean Sea. J. Plankton Res. 23:1297-1310
    • (2001) J. Plankton Res. , vol.23 , pp. 1297-1310
    • Christaki, U.1    Giannakourou, A.2    Van Wambeke, F.3    Gregori, G.4
  • 7
    • 22744439762 scopus 로고    scopus 로고
    • Grazing impact of different heterotrophic nanoflagellates on eukaryotic (Ostreococcus tauri) and prokaryotic picoautotrophs (Prochlorococcus and Synechococcus)
    • Christaki, U., E. Vázquez-Domínguez, C. Courties, and P. Lebaron. 2005. Grazing impact of different heterotrophic nanoflagellates on eukaryotic (Ostreococcus tauri) and prokaryotic picoautotrophs (Prochlorococcus and Synechococcus). Envir. Microbiol. 7(8):1200-1210
    • (2005) Envir. Microbiol. , vol.7 , Issue.8 , pp. 1200-1210
    • Christaki, U.1    Vázquez-Domínguez, E.2    Courties, C.3    Lebaron, P.4
  • 8
    • 0029789243 scopus 로고    scopus 로고
    • Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13
    • del Giorgio, P. A., D. F. Bird, Y. T. Prairie, and D. Planas. 1996. Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13. Limnol. Oceanogr. 41:783-789
    • (1996) Limnol. Oceanogr. , vol.41 , pp. 783-789
    • del Giorgio, P.A.1    Bird, D.F.2    Prairie, Y.T.3    Planas, D.4
  • 9
    • 0032486094 scopus 로고    scopus 로고
    • DNA damage caused by common cytological fixatives
    • Douglas, M. P., and S. O. Rogers. 1998. DNA damage caused by common cytological fixatives. Mutat. Res. 401:77-88
    • (1998) Mutat. Res. , vol.401 , pp. 77-88
    • Douglas, M.P.1    Rogers, S.O.2
  • 10
    • 0032829065 scopus 로고    scopus 로고
    • Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria
    • Gasol, J. P., U. L. Zweifel, F.Peters, J. A. Fuhrman, and Å. Hagström. 1999. Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl. Environ. Microbiol. 65:4475-4483
    • (1999) Appl. Environ. Microbiol. , vol.65 , pp. 4475-4483
    • Gasol, J.P.1    Zweifel, U.L.2    Peters, F.3    Fuhrman, J.A.4    Hagström, Å.5
  • 11
    • 0033865159 scopus 로고    scopus 로고
    • Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities
    • Gasol, J. M., and P. A. Del Giorgio. 2000. Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci. Mar. 64:197-224.
    • (2000) Sci. Mar. , vol.64 , pp. 197-224
    • Gasol, J.M.1    Del Giorgio, P.A.2
  • 12
    • 0001412719 scopus 로고    scopus 로고
    • Sampling, preservation, enumeration and biomass of marine protozooplankton
    • Academic Press, R. P. Harris, P. H. Wiebe, J. Lenz, H. R. Skjoldal, and M. Huntley [eds.]
    • Gifford, D. J., and D. A. Caron. 2000. Sampling, preservation, enumeration and biomass of marine protozooplankton, p. 193-221. In R. P. Harris, P. H. Wiebe, J. Lenz, H. R. Skjoldal, and M. Huntley [eds.], ICES zooplankton methodology manual. Academic Press.
    • (2000) ICES zooplankton methodology manual , pp. 193-221
    • Gifford, D.J.1    Caron, D.A.2
  • 15
    • 0031596935 scopus 로고    scopus 로고
    • Comparison of blue nucleic acid dyes fot flow cytometric enumeration of bacteria in aquatic systems
    • Lebaron, P., N. Parthuisot, and P. Catala. 1998. Comparison of blue nucleic acid dyes fot flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol. 64:1725-1730.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 1725-1730
    • Lebaron, P.1    Parthuisot, N.2    Catala, P.3
  • 16
    • 0028163796 scopus 로고
    • Primary productivity of Prochlorophytes, cyanobacteria, and eukaryotic ultra-plankton: measurements from flow cytometric sorting
    • Li, W. K. W. 1994. Primary productivity of Prochlorophytes, cyanobacteria, and eukaryotic ultra-plankton: measurements from flow cytometric sorting. Limnol. Oceanogr. 39:169-175
    • (1994) Limnol. Oceanogr. , vol.39 , pp. 169-175
    • Li, W.K.W.1
  • 17
    • 0028995826 scopus 로고
    • DNA distribution in planktonic bacteria stained with TOTO or TO-PRO
    • Li, W. K. W, J. F. Jellet, and P. M. Dickie. 1995. DNA distribution in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr. 40:1485-1495
    • (1995) Limnol. Oceanogr. , vol.40 , pp. 1485-1495
    • Li, W.K.W.1    Jellet, J.F.2    Dickie, P.M.3
  • 18
    • 0036164779 scopus 로고    scopus 로고
    • Enumeration of small ciliates in culture by flow cytometry and nucleic acid staining
    • Lindström, E. S., T.Weisse, and P. Stadler. 2002. Enumeration of small ciliates in culture by flow cytometry and nucleic acid staining. J. Microbiol. Meth. 49:173-182
    • (2002) J. Microbiol. Meth. , vol.49 , pp. 173-182
    • Lindström, E.S.1    Weisse, T.2    Stadler, P.3
  • 19
    • 0029883988 scopus 로고    scopus 로고
    • Application of the novel nucleic acid dyes YOYO-1 YOPRO-1 and PicoGreen for flow cytometric analysis of marine prokaryotes
    • Marie, D., D. Vaulot, and F. Partensky. 1996. Application of the novel nucleic acid dyes YOYO-1, YOPRO-1 and PicoGreen for flow cytometric analysis of marine prokaryotes. Appl. Environ. Microbiol. 62:1649-1655.
    • (1996) Appl. Environ. Microbiol. , vol.62 , pp. 1649-1655
    • Marie, D.1    Vaulot, D.2    Partensky, F.3
  • 20
    • 0030620443 scopus 로고    scopus 로고
    • Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I
    • Marie, D.,F. Partensky, S. Jacquet, and D. Vaulot. 1997. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol. 63:186-193.
    • (1997) Appl. Environ. Microbiol. , vol.63 , pp. 186-193
    • Marie, D.1    Partensky, F.2    Jacquet, S.3    Vaulot, D.4
  • 21
    • 0002499755 scopus 로고    scopus 로고
    • Flow cytometric analysis of phytoplankton, bacteria and viruses
    • John Wiley and Sons, J. Robinson [eds.]
    • Marie, D.,C. Brussaard, F. Partensky, and D. Vaulot. 1999. Flow cytometric analysis of phytoplankton, bacteria and viruses, p. 1-15. In J. Robinson [eds.], Current protocols in cytometry. John Wiley and Sons.
    • (1999) Current protocols in cytometry , pp. 1-15
    • Marie, D.1    Brussaard, C.2    Partensky, F.3    Vaulot, D.4
  • 23
    • 33746757776 scopus 로고    scopus 로고
    • Distribution and abundance of uncultured heterotrophic flagellates in the world oceans
    • Massana, A., R. Terrado, I. Forn, C. Lovejoy, and C. Pedros-Allió. 2006. Distribution and abundance of uncultured heterotrophic flagellates in the world oceans. Envir. Microbiol. 8(9):1515-1522
    • (2006) Envir. Microbiol. , vol.8 , Issue.9 , pp. 1515-1522
    • Massana, A.1    Terrado, R.2    Forn, I.3    Lovejoy, C.4    Pedros-Allió, C.5
  • 24
    • 0000534428 scopus 로고
    • Size-selective grazing by heterotrophic flagellates: An analysis using live-stained bacteria and dual-beam flow cytometry
    • Monger, B. C. and M. R. Landry. 1992. Size-selective grazing by heterotrophic flagellates: An analysis using live-stained bacteria and dual-beam flow cytometry. Arch. Hydrobiol. Beih. Ergebn. Limnol. 37:173-185.
    • (1992) Arch. Hydrobiol. Beih. Ergebn. Limnol. , vol.37 , pp. 173-185
    • Monger, B.C.1    Landry, M.R.2
  • 25
    • 0027466710 scopus 로고
    • Flow cytometric analysis of marine bacteria with Hoechst 33342
    • Monger, B. C. and M. R. Landry. 1993. Flow cytometric analysis of marine bacteria with Hoechst 33342. Appl. Environ. Microbiol. 59:905-911.
    • (1993) Appl. Environ. Microbiol. , vol.59 , pp. 905-911
    • Monger, B.C.1    Landry, M.R.2
  • 26
    • 0001526699 scopus 로고
    • Phytoplankton analysis using flow cytometry
    • Lewis, P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. Cole, [eds.]
    • Olson, R. J., E. R. Zettler, and M. D. DuRand. 1993. Phytoplankton analysis using flow cytometry, p 175-186. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. Cole, [eds.]. Handbook of methods in aquatic microbial ecology. Lewis.
    • (1993) Handbook of methods in aquatic microbial ecology , pp. 175-186
    • Olson, R.J.1    Zettler, E.R.2    DuRand, M.D.3
  • 27
    • 0025526837 scopus 로고
    • Planktonic community structure determines the fate of bacterial production in a temperate lake
    • Pace, M., G. B. McManus, and S. G. Findlay. 1990. Planktonic community structure determines the fate of bacterial production in a temperate lake. Limnol. Oceanogr. 35:795-808
    • (1990) Limnol. Oceanogr. , vol.35 , pp. 795-808
    • Pace, M.1    McManus, G.B.2    Findlay, S.G.3
  • 28
    • 4644226321 scopus 로고    scopus 로고
    • Simultaneous fluorescence in situ hybridization of mRNA and rRNA in environmental bacteria
    • Pernthaler, A., and R. Amann. 2004. Simultaneous fluorescence in situ hybridization of mRNA and rRNA in environmental bacteria. Appl. Envir. Microbiol. 70:5426-5433
    • (2004) Appl. Envir. Microbiol. , vol.70 , pp. 5426-5433
    • Pernthaler, A.1    Amann, R.2
  • 29
    • 0027369798 scopus 로고
    • Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting
    • Porter, J., C. Edwards, A. W. Morgan, and R. W. Pickup. 1993. Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting. Appl. Environ. Microbiol. 59:3327-3333.
    • (1993) Appl. Environ. Microbiol. , vol.59 , pp. 3327-3333
    • Porter, J.1    Edwards, C.2    Morgan, A.W.3    Pickup, R.W.4
  • 30
    • 84986870589 scopus 로고
    • The use of DAPI for identifying and counting aquatic microflora
    • Porter, K. G., and Y. S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943-948
    • (1980) Limnol. Oceanogr. , vol.25 , pp. 943-948
    • Porter, K.G.1    Feig, Y.S.2
  • 31
    • 0024595761 scopus 로고
    • Characterizing aquatic bacteria according to population, cell size and apparent DNA content by flow cytometry
    • Robertson, B. R., and D. K. Button. 1989. Characterizing aquatic bacteria according to population, cell size and apparent DNA content by flow cytometry. Cytometry 10:70-76
    • (1989) Cytometry 10 , pp. 70-76
    • Robertson, B.R.1    Button, D.K.2
  • 32
    • 4143140881 scopus 로고    scopus 로고
    • Counting heterotrophic nanoplanktonic protists in cultures aquatic communities by flow cytometry
    • Rose, J. M., D. A. Caron, M. E. Sieraki, and N. Poulton. 2004. Counting heterotrophic nanoplanktonic protists in cultures aquatic communities by flow cytometry. Aquat. Microb. Ecol. 34:263-277
    • (2004) Aquat. Microb. Ecol. , vol.34 , pp. 263-277
    • Rose, J.M.1    Caron, D.A.2    Sieraki, M.E.3    Poulton, N.4
  • 33
    • 0034640528 scopus 로고    scopus 로고
    • Structure of the oligotrophic planktonic food web under low grazing of heterotrophic bacteria: Takapoto Atoll
    • Sakka, A., L. Legendre, M. Gosselin, and B. Delesalle. 2000. Structure of the oligotrophic planktonic food web under low grazing of heterotrophic bacteria: Takapoto Atoll, French Polynesia. Mar. Ecol. Prog. Ser. 197:1-17
    • (2000) French Polynesia. Mar. Ecol. Prog. Ser. , vol.197 , pp. 1-17
    • Sakka, A.1    Legendre, L.2    Gosselin, M.3    Delesalle, B.4
  • 34
    • 0024936987 scopus 로고
    • Seasonal patterns of bacterivory by flagellates, ciliates, rotifers, and cladocerans in a freshwater planktonic community
    • Sanders, R., K. G. Porter, S. J. Bennet, and A. E. Debiase. 1989. Seasonal patterns of bacterivory by flagellates, ciliates, rotifers, and cladocerans in a freshwater planktonic community. Limnol. Oceanogr. 34:673-687
    • (1989) Limnol. Oceanogr. , vol.34 , pp. 673-687
    • Sanders, R.1    Porter, K.G.2    Bennet, S.J.3    Debiase, A.E.4
  • 36
    • 0000693782 scopus 로고
    • Trophic roles of pelagic protists: phagotrophic flagellates as herbivores
    • Sherr, E. B., and B. F. Sherr. 1992. Trophic roles of pelagic protists: phagotrophic flagellates as herbivores. Arch. Hydrobiol. Beih. 37:165-172.
    • (1992) Arch. Hydrobiol. Beih. , vol.37 , pp. 165-172
    • Sherr, E.B.1    Sherr, B.F.2
  • 37
    • 0001401636 scopus 로고
    • Preservation and storage of samples for enumeration of heterotrophic protists
    • Lewis, P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. Cole [eds.]
    • Sherr, E. B., and B. F. Sherr. 1993. Preservation and storage of samples for enumeration of heterotrophic protists, p.207-212. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. Cole [eds.], Handbook of methods in aquatic microbial ecology. Lewis.
    • (1993) Handbook of methods in aquatic microbial ecology , pp. 207-212
    • Sherr, E.B.1    Sherr, B.F.2
  • 38
    • 15844367939 scopus 로고
    • Bacterivory and herbivory: key roles of phagotrophic protists in pelagic food webs
    • Sherr, E. B., and B. F. Sherr. 1994. Bacterivory and herbivory: key roles of phagotrophic protists in pelagic food webs. Microb. Ecol. 28:223-235
    • (1994) Microb. Ecol. , vol.28 , pp. 223-235
    • Sherr, E.B.1    Sherr, B.F.2
  • 39
    • 0036422782 scopus 로고    scopus 로고
    • Significant of predation by protists in aquatic microbial food webs
    • Sherr, E. B., and B. F. Sherr. 2002. Significant of predation by protists in aquatic microbial food webs. Anton. Leeuv. Int. J. G. 81:293-308
    • (2002) Anton. Leeuv. Int. J. G. , vol.81 , pp. 293-308
    • Sherr, E.B.1    Sherr, B.F.2
  • 40
    • 78049417224 scopus 로고    scopus 로고
    • Community heterogeneity and single-cell digestive activity of estuarine heterotrophic nanoflagelattes assed using lysotracker and flow cytometry
    • Sintes, E., and P. A. del Giorgio. 2010. Community heterogeneity and single-cell digestive activity of estuarine heterotrophic nanoflagelattes assed using lysotracker and flow cytometry. Environ. Microb. 12:1913-1925
    • (2010) Environ. Microb. , vol.12 , pp. 1913-1925
    • Sintes, E.1    del Giorgio, P.A.2
  • 41
    • 0028885164 scopus 로고
    • Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: Fixation and storage effects
    • Troussellier, M., C. Courties, and S. Zettelmaier. 1995. Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: Fixation and storage effects. Estuar. Coast. Shelf Sci. 40:621-633
    • (1995) Estuar. Coast. Shelf Sci. , vol.40 , pp. 621-633
    • Troussellier, M.1    Courties, C.2    Zettelmaier, S.3
  • 42
    • 0032814029 scopus 로고    scopus 로고
    • Flow cytometric discrimination of bacterial populations in seawater based on SYTO 13 staining of nucleic acids
    • Troussellier, M., C. Courties, P. Lebaron, and P. Servais. 1999. Flow cytometric discrimination of bacterial populations in seawater based on SYTO 13 staining of nucleic acids. FEMS Microbiol. Ecol. 29:319-330
    • (1999) FEMS Microbiol. Ecol. , vol.29 , pp. 319-330
    • Troussellier, M.1    Courties, C.2    Lebaron, P.3    Servais, P.4
  • 43
    • 0024728767 scopus 로고
    • A simple method to preserve oceanic phytoplankton for flow cytometric analyses
    • Vaulot, D., C. Courties, and F. Partensky. 1989. A simple method to preserve oceanic phytoplankton for flow cytometric analyses. Cytometry 10:629-635
    • (1989) Cytometry , vol.10 , pp. 629-635
    • Vaulot, D.1    Courties, C.2    Partensky, F.3
  • 44
    • 0030678621 scopus 로고    scopus 로고
    • Flow sorting of microorganisms for molecular analysis
    • Wallner, G., B. Fuchs, and K. Jurgens. 1997. Flow sorting of microorganisms for molecular analysis. Appl. Envir. Microbiol. 63:4223-4231.
    • (1997) Appl. Envir. Microbiol. , vol.63 , pp. 4223-4231
    • Wallner, G.1    Fuchs, B.2    Jurgens, K.3
  • 46
    • 0034841015 scopus 로고    scopus 로고
    • Digestion of bacterial macromolecules by a mixotrophic flagellate, Ochromonas sp., compared with that by two heterotrophic flagellates, Spumella pudica and Bodo saltans
    • Zubkov, M. V., E. Zollner, and K. Jurgens. 2001. Digestion of bacterial macromolecules by a mixotrophic flagellate, Ochromonas sp., compared with that by two heterotrophic flagellates, Spumella pudica and Bodo saltans. Eur. J. Protistol. 37:155-166
    • (2001) Eur. J. Protistol. , vol.37 , pp. 155-166
    • Zubkov, M.V.1    Zollner, E.2    Jurgens, K.3
  • 47
    • 24944542802 scopus 로고    scopus 로고
    • Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas: Assessment using flow cytometric sorting
    • Zubkov, M. V., and M. A. Sleigh. 2005. Assimilation efficiency of Vibrio bacterial protein biomass by the flagellate Pteridomonas: Assessment using flow cytometric sorting. FEMS Microbiol. Ecol. 54:281-286
    • (2005) FEMS Microbiol. Ecol. , vol.54 , pp. 281-286
    • Zubkov, M.V.1    Sleigh, M.A.2
  • 48
    • 33749472206 scopus 로고    scopus 로고
    • Syringe pumped high speed flow cytometry of oceanic phytoplankton
    • Zubkov, M. V., and P. Burkill. 2006. Syringe pumped high speed flow cytometry of oceanic phytoplankton. Cytometry A 69A:1010-1019
    • (2006) Cytometry A , vol.69 A , pp. 1010-1019
    • Zubkov, M.V.1    Burkill, P.2
  • 49
    • 34047216390 scopus 로고    scopus 로고
    • Flow cytometric enumeration of DNA-stained oceanic planktonic protists
    • Zubkov, M. V., P. Burkill, and J. N. Topping. 2007. Flow cytometric enumeration of DNA-stained oceanic planktonic protists. J. Plank. Res. 29:79-86
    • (2007) J. Plank. Res. , vol.29 , pp. 79-86
    • Zubkov, M.V.1    Burkill, P.2    Topping, J.N.3


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