Enhanced production of periplasmic interferon alpha-2b by Escherichia coli using ion-exchange resin for in situ removal of acetate in the culture

Biochemical Engineering Journal

Volumn 58-59, Issue 1, 2011, Pages 124-132

  Tan, Joo Shun ^a   Ramanan, Ramakrishnan Nagasundara ^b   Ling, Tau Chuan ^c   Shuhaimi, Mustafa ^a   Ariff, Arbakariya B ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b MONASH UNIVERSITY MALAYSIA   (Malaysia)

^c UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Acetic acid; Adsorption; Anion exchange resins; Bioreactors; Fermentation; Periplasmic interferon alpha2b

Indexed keywords

ANION EXCHANGE RESINS; E. COLI; HIGH CONCENTRATION; IN-SITU; INHIBITORY EFFECT; PERIPLASMIC INTERFERON-ALPHA2B; RESPONSE SURFACE METHODOLOGY; SHAKE-FLASK CULTURES; STIRRED TANK BIOREACTORS; WEAK BASE;

ACETIC ACID; ACTINIDES; ADSORPTION; ANTIBIOTICS; BIOCONVERSION; BIOREACTORS; CELL CULTURE; ESCHERICHIA COLI; FERMENTATION; GLYCOPROTEINS; ION EXCHANGE RESINS; POSITIVE IONS; TANKS (CONTAINERS); VOLATILE FATTY ACIDS;

ION EXCHANGE;

ACETIC ACID; ALPHA2B INTERFERON; ANION EXCHANGE RESIN; ION EXCHANGE RESIN; PERIPLASMIC ALPHA2B INTERFERON; UNCLASSIFIED DRUG;

ABSORPTION; ARTICLE; BACTERIAL GROWTH; ESCHERICHIA COLI; FERMENTATION; GENE SEQUENCE; INTERFERON PRODUCTION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; STIRRED REACTOR;

ESCHERICHIA COLI;

EID: 80054837545     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2011.08.018     Document Type: Article

References (33)

1. Perrillo R.P., Schiff E.R., Davis G.L., Bodenheimer H.C., Lindsay K., Payne J., Dienstag J.L., O'Brien C., Tamburro C., Jacobson I.M. A randomized, controlled trial of interferon alfa-2b alone and after prednisone withdrawal for the treatment of chronic hepatitis B. N. Engl. J. Med. 1990, 323:295-301.
2. Chen Y.H., Stoiber H., Dierich M.P. Increased levels of antibodies against interferon-[alpha] in HIV-1 positive individuals may be explained by a common immunological epitope on the human interferon-[alpha] and HIV-1 gp41. Immunol. Lett. 1997, 55:15-18.
3. Golomb H.M., Jacobs A., Fefer A., Ozer H., Thompson J., Portlock C., Ratain M., Golde D., Vardiman J., Burke J.S. Alpha-2 interferon therapy of hairy-cell leukemia: a multicenter study of 64 patients. J. Clin. Oncol. 1986, 4:900.
4. Eiteman M.A., Altman E. Overcoming acetate in Escherichia coli recombinant protein fermentations. Trends Biotechnol. 2006, 24:530-536.
5. Ishizaki A., Ueda T., Tanaka K., Stanbury P.F. The kinetics of end-product inhibition of l-lactate production from xylose and glucose by Lactococcus lactis IO-1. Biotechnol. Lett. 1993, 15:489-494.
6. Li Q., Li W.-l., Wang D., Liu B.-b., Tang H., Yang M.-h., Liu Q.-f., Xing J.-m., Su Z.-g. pH Neutralization while succinic acid adsorption onto anion-exchange resins. Appl. Biochem. Biotechnol. 2008.
7. Weiner N., Draskoczy P. The effects of organic acids on the oxidative metabolism of intact and disrupted E. coli. J. Pharmacol. Exp. Ther. 1961, 132:299-305.
8. Slonczewski J.L., Rosen B.P., Alger J.R., Macnab R.M. pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate. Proc. Natl. Acad. Sci. U.S.A. 1981, 78:6271-6275.
9. Slonczewski J.L., Macnab R.M., Alger J.R., Castle A.M. Effects of pH and repellent tactic stimuli on protein methylation levels in Escherichia coli. J. Bacteriol. 1982, 152:384-399.
10. Wilks J.C., Slonczewski J.L. pH of the cytoplasm and periplasm of Escherichia coli: rapid measurement by green fluorescent protein fluorimetry. J. Bacteriol. 2007, 189:5601-5607.
11. Luli G.W., Strohl W.R. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 1990, 56:1004-1011.
12. Meyer H.-P., Leist C., Fiechter A. Acetate formation in continuous culture of Escherichia coli K12 D1 on defined and complex media. J. Biotechnol. 1984, 1:355-358.
13. Turner C., Gregory M.E., Turner M.K. A study of the effect of specific growth rate and acetate on recombinant protein production of Escherichia coli JM107. Biotechnol. Lett. 1994, 16:891-896.
14. Chen rsquo ai X., Cen P., Chen J. Enhanced production of human epidermal growth factor by a recombinant Escherichia coli integrated with in situ exchange of acetic acid by macroporous ion-exchange resin. Soc. Biotechnol. Jpn. 2005, 100:579-581.
15. Aristos A., San K.Y., Bennett G.N. Modification of central metabolic pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene. Biotechnol. Bioeng. 1994, 44:944-951.
16. Ponce E. Effect of growth rate reduction and genetic modifications on acetate accumulation and biomass yields in Escherichia coli. J. Biosci. Bioeng. 1999, 87:775-780.
17. Luo Q.P., Shen Y.-L., Wei D.-Z., Cao W. Optimization of culture on the overproduction of TRAIL in high-cell-density culture by recombinant Escherichia coli. Appl. Microbiol. Biotechnol. 2006, 71:184-191.
18. Shiloach J., Kaufman J., Guillard A.S., Fass R. Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (lambdaDE3) and Escherichia coli JM109. Biotechnol. Bioeng. 1996, 49:421-428.
19. Wickramasinghe S.R., Grzenia D.L. Adsorptive membranes and resins for acetic acid removal from biomass hydrolysates. Desalination 2008, 234:144-151.
20. Wong M., Wright M., Woodley J.M., Lye G.J. Enhanced recombinant protein synthesis in batch and fed batch Escherichia coli fermentation based on removal of inhibitory acetate by electrodialysis. J. Chem. Technol. Biotechnol. 2009, 84:1284-1291.
21. Ramanan R.N., Tik W.B., Memari H.R., Azaman S.N.A., Ling T.C., Tey B.T., Lila M.A.M., Abdullah M.P., Rahim R.A., Ariff A.B. Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-2b in Escherichia coli. Afr. J. Biotechnol. 2010, 9:285-292.
22. Tan J.S., Ramanan R.N., Azaman S.N.A., Ling T.C., Shuhaimi M., Ariff A.B. Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method. J. Open Biotechnol. 2009.
23. Ramanan R.N., Tan J.S., Mohamed M.S., Ling T.C., Tey B.T., Ariff A.B. Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-[alpha] 2b from Escherichia coli using response surface method. Process Biochem. 2010, 45:196-202.
24. Ramanan R.N., Ling T.C., Tey B.T., Ariff A.B. A simple method for quantification of interferon-2b through surface plasmon resonance technique. Afr. J. Biotechnol. 2010, 9:1680-1689.
25. Azaman S., Ramakrishnan N., Tan J., Rahim R., Abdullah M., Ariff A. Optimization of an induction strategy for improving interferon-alpha2b production in the periplasm of Escherichia coli using response surface methodology. Biotechnol. Appl. Biochem. 2010, 56:141-150.
26. Han K., Lim H.C., Hong J. Acetic acid formation in Escherichia coli fermentation. Biotechnol. Bioeng. 1992, 39:663-671.
27. Kazan D., Camurdan A., Hortacsu A. The effect of glucose concentration on the growth rate and some intracellular components of a recombinant E. coli culture. Process Biochem. 1995, 30:269-273.
28. Panda A.K., Khan R.H., Mishra S., Appa Rao K.B.C., Totey S.M. Influences of yeast extract on specific cellular yield of Ovine growth hormone during fed-batch fermentation of E. coli. Bioprocess Biosyst. Eng. 2000, 22:379-383.
29. Rotman B. Uses of ion exchange resins in microbiology. Microbiol. Mol. Biol. Rev. 1960, 24:251.
30. Singh S.M., Panda A.K. Solubilization and refolding of bacterial inclusion body proteins. J. Biosci. Bioeng. 2005, 99:303-310.
31. Kleman G.L., Strohl W.R. Developments in high cell density and high productivity microbial fermentation. Curr. Opin. Biotechnol. 1994, 5:180-186.
32. Tripathi N.K., Sathyaseelan K., Jana A.M., Rao P.V.L. High yield production of heterologous proteins with Escherichia coli. Def. Sci. J. 2009, 59:137-146.
33. Shin C.S., Hong M.S., Shin H.C., Lee J. High-level production of recombinant human IFN-2a with co-expression of tRNA Arg (AGG/AGA) in high-cell-density cultures of Escherichia coli. Biotechnol. Bioprocess Eng. 2001, 6:301-305.