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Volumn 21, Issue 21, 2011, Pages 6482-6484

Protective effects of amentoflavone on Lamin A-dependent UVB-induced nuclear aberration in normal human fibroblasts

Author keywords

A; Aging; Amentoflavone; Lamin

Indexed keywords

AMENTOFLAVONE; HISTONE H2AX; LAMIN A;

EID: 80054783464     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.08.067     Document Type: Article
Times cited : (27)

References (14)
  • 9
    • 85030490298 scopus 로고    scopus 로고
    • The methanol extract was uspended in water and then partitioned by dichloromethane ethyl acetate and n-butanol in turn Three grams of the EtOAc fraction was subjected to olumn chromatography over a silica gel 300 g 4.8 cm×45 cm eluting with a HCl3-MeOH-H2O 12:1:0.1→8:1:0.1→5:1:0. 1→2:1:0.1→1:1:0.1→MeOH only gradient ystem Fractionswere combined based ntheir TLC pattern to yield ubfraction designated as E1-E10. SubfractionE7 296.33 mg was finally urified by repeated column chromatography over a silica gel RP-18 and ephadex LH 20 to afford amentoflavone 82.23 mg
    • Briefly the whole plant of S. tamariscina (600 g) was extracted with methanol at room temperature to afford 50.54 g of residue. The methanol extract was suspended in water and then partitioned by dichloromethane, ethyl acetate, and n-butanol in turn. Three grams of the EtOAc fraction was subjected to column chromatography over a silica gel (300 g, 4.8 cm × 45 cm) eluting with a CHCl3-MeOH-H2O (12:1:0.1→8:1:0.1→5:1:0.1→2:1:0.1→1:1:0. 1→MeOH only) gradient system. Fractions were combined based on their TLC pattern to yield subfraction designated as E1-E10. Subfraction E7 (296.33 mg) was finally purified by repeated column chromatography over a silica gel, RP-18, and Sephadex LH 20 to afford amentoflavone (82.23 mg).
    • Briefly the Whole Plant of S. Tamariscina 600 g was Extracted with Methanol at Room Temperature to Afford 50.54 g of Residue
  • 11
    • 85030497018 scopus 로고    scopus 로고
    • Cells were maintained in culture medium with 1% Fetal Bovine Serum FBS and amentoflavone at the indicated concentration for 24 h. Pre-treated cells were washed twice with phosphate-buffered saline PBS Welgene Daegu Korea and exposed to UVB irradiation UVB lamp: G15TBE SANKYO DENKI Japan
    • Cells were maintained in culture medium with 1% Fetal Bovine Serum (FBS) and amentoflavone at the indicated concentration for 24 h. Pre-treated cells were washed twice with phosphate-buffered saline (PBS) (Welgene, Daegu, Korea) and exposed to UVB irradiation (UVB lamp: G15TBE, SANKYO DENKI, Japan). The total energy dose of UVB irradiation was 100 mJ/cm2.
    • The total energy dose of UVB irradiation was 100 mJ/cm2
  • 13
    • 0024324285 scopus 로고
    • Cells were Grown on Eight Well Chamber Slides Lab-Tek Chamber Slide System
    • Kligman, L. H. J. Am. Acad. Dermatol. 1989, 21, 623.
    • (1989) J. Am. Acad. Dermatol. , vol.21 , pp. 623
    • Kligman, L.H.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.