A Proteomic Protocol to Identify Physiological Substrates of Pro-protein Convertases

Methods in Molecular Biology

Volumn 768, Issue , 2011, Pages 325-341

  Nie, Guiying ^a   Stephens, Andrew N ^a  

^a HUDSON INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

2D DiGE; PC; PC6; Proprotein convertase; proteomics; substrate

Indexed keywords

PROPROTEIN CONVERTASE 5; PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; DECIDUA; ENDOMETRIUM; ENZYMOLOGY; FEMALE; HUMAN; METABOLISM; METHODOLOGY; NIDATION; PHYSIOLOGY; PROTEIN PROCESSING; PROTEOMICS; STROMA CELL; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

ANIMALS; CELL CULTURE TECHNIQUES; DECIDUA; EMBRYO IMPLANTATION; ENDOMETRIUM; FEMALE; HUMANS; PROPROTEIN CONVERTASE 5; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEINS; PROTEOMICS; STROMAL CELLS; TANDEM MASS SPECTROMETRY; TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS;

EID: 80054763780     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-204-5_18     Document Type: Chapter

References (12)

1. Seidah, N. G., Day, R., Marcinkiewicz, M., and Chretien, M. (1998) Precursor convertases: An evolutionary ancient, cell-specific, combinatorial mechanism yielding diverse bioactive peptides and proteins Ann NY Acad Sci 839, 9–24.
2. Khatib, A. M., Siegfried, G., Chretien, M., Metrakos, P., and Seidah, N. G. (2002) Proprotein convertases in tumor progression and malignancy: Novel targets in cancer therapy Am J Pathol 160, 1921–35.
3. Taylor, N. A., Van De Ven, W. J., and Creemers, J. W. (2003) Curbing activation: Proprotein convertases in homeostasis and pathology FASEB J 17, 1215–27.
4. Nakayama, K., Watanabe, T., Nakagawa, T., Kim, W. S., Nagahama, M., Hosaka, M., Hatsuzawa, K., Kondoh-Hashiba, K., and Murakami, K. (1992) Consensus sequence for precursor processing at mono-arginyl sites. Evidence for the involvement of a Kex2-like endoprotease in precursor cleavages at both dibasic and mono-arginyl sites J Biol Chem 267, 16335–40.
5. Watanabe, T., Nakagawa, T., Ikemizu, J., Nagahama, M., Murakami, K., and Nakayama, K. (1992) Sequence requirements for precursor cleavage within the constitutive secretory pathway J Biol Chem 267, 8270–4.
6. Seidah, N. G., Mayer, G., Zaid, A., Rousselet, E., Nassoury, N., Poirier, S., Essalmani, R., and Prat, A. (2008) The activation and physiological functions of the proprotein convertases Int J Biochem Cell Biol 40, 1111–25.
7. Seidah, N. G., and Chretien, M. (1999) Proprotein and prohormone convertases: A family of subtilases generating diverse bioactive polypeptides Brain Res 848, 45–62.
8. Bredemeyer, A. J., Lewis, R. M., Malone, J. P., Davis, A. E., Gross, J., Townsend, R. R., and Ley, T. J. (2004) A proteomic approach for the discovery of protease substrates Proc Natl Acad Sci USA 101, 11785–90.
9. Kilpatrick, L. M., Stephens, A. N., Hardman, B. M., Salamonsen, L. A., Li, Y., Stanton, P. G., and Nie, G. (2009) Proteomic identification of caldesmon as a physiological substrate of proprotein convertase 6 in human uterine decidual cells essential for pregnancy establishment J Proteome Res 8, 4983–92.
10. Nie, G., Li, Y., Wang, M., Liu, Y. X., Findlay, J. K., and Salamonsen, L. A. (2005) Inhibiting uterine PC6 blocks embryo implantation: An obligatory role for a proprotein convertase in fertility Biol Reprod 72, 1029–36.
11. Okada, H., Nie, G., and Salamonsen, L. A. (2005) Requirement for proprotein convertase 5/6 during decidualization of human endometrial stromal cells in vitro J Clin Endocrinol Metab 90, 1028–34.
12. Duckert, P., Brunak, S., and Blom, N. (2004) Prediction of proprotein convertase cleavage sites Protein Eng Des Sel 17, 107–12.