Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - A comparison of culture-dependent and culture-independent methods

BMC Microbiology

Volumn 11, Issue , 2011, Pages

  Pitkäranta, Miia ^a   Meklin, Teija ^b,c   Hyvärinen, Anne ^b   Nevalainen, Aino ^b   Paulin, Lars ^a   Auvinen, Petri ^a   Lignell, Ulla ^b   Rintala, Helena ^b,c  

^a UNIVERSITY OF HELSINKI   (Finland)

^b NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

^c Mikrobioni Inc   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

INTERNAL TRANSCRIBED SPACER; WATER;

ARTICLE; ASCOMYCETES; BASIDIOMYCETES; BIOREMEDIATION; BUILDING; BUILDING MATERIAL; DUST; FUNGAL COMMUNITY; FUNGUS CULTURE; MICROBIAL DIVERSITY; MOISTURE; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; SPECIES CULTIVATION; YEAST; BACTERIAL COUNT; BIODIVERSITY; CLASSIFICATION; COMPARATIVE STUDY; FUNGUS; GENETICS; GROWTH, DEVELOPMENT AND AGING; HOUSING; HUMAN; HUMIDITY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PHYLOGENY;

DOTHIDEOMYCETES; FUNGI; MYCOTA;

BIODIVERSITY; COLONY COUNT, MICROBIAL; CONSTRUCTION MATERIALS; DUST; FUNGI; HOUSING; HUMANS; HUMIDITY; PHYLOGENY; POLYMERASE CHAIN REACTION;

EID: 80054759099     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-11-235     Document Type: Article

References (66)

1. Respiratory and allergic health effects of dampness, mold, and dampness-related agents: a review of the epidemiologic evidence. Mendell MJ, Mirer AG, Cheung K, Tong M, Douwes J, J Environ Health Perspect 2011 119 748 756 10.1289/ehp.1002410
2. Meta-analyses of the associations of respiratory health effects with dampness and mold in homes. Fisk WJ, Lei-Gomez Q, Mendell MJ, Indoor Air 2007 17 284 296 10.1111/j.1600-0668.2007.00475.x 17661925 (Pubitemid 47170117)
3. Public health and economic impact of dampness and mold. Mudarri D, Fisk WJ, Indoor Air 2007 17 226 235 10.1111/j.1600-0668.2007.00474.x 17542835 (Pubitemid 46836586)
4. Comparison of indoor fungal spore levels before and after professional home remediation. Barnes CS, Dowling P, Van Osdol T, Portnoy J, Ann Allergy Asthma Immunol 2007 98 262 268 10.1016/S1081-1206(10)60716-8 17378258 (Pubitemid 46417572)
5. Building-related symptoms and molds: a two-step intervention study. Ebbehoj NE, Hansen MO, Sigsgaard T, Larsen L, Indoor Air 2002 12 273 277 10.1034/j.1600-0668.2002.02141.x 12532759 (Pubitemid 135747827)
6. Estimating effects of moisture damage repairs on students' health-a long-term intervention study. Haverinen-Shaughnessy U, Pekkanen J, Nevalainen A, Moschandreas D, Husman T, J Expo Anal Environ Epidemiol 2004 14 Suppl 1 58 64 15118746
7. Reduction in asthma morbidity in children as a result of home remediation aimed at moisture sources. Kercsmar CM, Dearborn DG, Schluchter M, Xue L, Kirchner HL, Sobolewski J, Greenberg SJ, Vesper SJ, Allan T, Environ Health Perspect 2006 114 1574 1580 10.1289/ehp.8742 17035145 (Pubitemid 44509657)
8. Effects of moisture damage and renovation on microbial conditions and pupils' health in two schools - a longitudinal analysis of five years. Lignell U, Meklin T, Putus T, Rintala H, Vepsalainen A, Kalliokoski P, Nevalainen A, J Environ Monit 2007 9 225 233 10.1039/b615459j 17344947 (Pubitemid 46365499)
9. The remediation of mold damaged school - a three-year follow-up study on teachers' health. Patovirta RL, Husman T, Haverinen U, Vahteristo M, Uitti JA, Tukiainen H, Nevalainen A, Cent Eur J Public Health 2004 12 36 42 15068207 (Pubitemid 38352044)
10. Respiratory morbidity among children following renovation of a water-damaged school. Savilahti R, Uitti J, Laippala P, Husman T, Roto P, Arch Environ Health 2000 55 405 410 10.1080/00039890009604038 11128878
11. Monitoring success of remediation: seven case studies of moisture and mold damaged buildings. Haverinen-Shaughnessy U, Hyvärinen A, Putus T, Nevalainen A, Sci Total Environ 2008 399 19 27 10.1016/j.scitotenv.2008.03.033 18455755
12. Effects of moisture-damage repairs on microbial exposure and symptoms in schoolchildren. Meklin T, Putus T, Pekkanen J, Hyvärinen A, Hirvonen MR, Nevalainen A, Indoor Air 2005 15 Suppl 10 40 47 15926943 (Pubitemid 40834241)
13. Dampness and mould. WHO guidelines for indoor air quality. World Health Organization, Copenhagen 2009 http://www.euro.who.int/-data/assets/pdf-file/ 0017/43325/E92645.pdf
14. Fungal spores: a critical review of the toxicological and epidemiological evidence as a basis for occupational exposure limit setting. Eduard W, Crit Rev Toxicol 2009 39 799 864 10.3109/10408440903307333 19863384
15. Health effects of indoor-air microorganisms. Husman T, Scand J Work Environ Health 1996 22 5 13 8685674 (Pubitemid 26090689)
16. Surveillance of fungal allergic sensitization using the fluorescent halogen immunoassay. Green BJ, Tovey ER, Beezhold DH, Perzanowski MS, Acosta LM, Divjan AI, Chew GL, J Med Mycol 2009 19 253 261
17. Chapter 4.1. Mycological investigations of indoor environments. Miller JD, Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control Boca Raton: CRC Press, Flannigan B, Samson RA, Miller JD, 2001 231 246
18. Microbial content of house dust samples determined with qPCR. Kaarakainen P, Rintala H, Vepsäläinen A, Hyvärinen A, Nevalainen A, Meklin T, Sci Total Environ 2009 407 4673 4680 10.1016/j.scitotenv.2009.04.046 19473690
19. Quantitative PCR analysis of house dust can reveal abnormal mold conditions. Meklin T, Haugland RA, Reponen T, Varma M, Lummus Z, Bernstein D, Wymer LJ, Vesper SJ, J Environ Monit 2004 6 615 620 10.1039/b400250d 15237292 (Pubitemid 38998229)
20. Development of an Environmental Relative Moldiness index for US homes. Vesper S, McKinstry C, Haugland R, Wymer L, Bradham K, Ashley P, Cox D, Dewalt G, Friedman W, J Occup Environ Med 2007 49 829 833 10.1097/JOM.0b013e3181255e98 17693779 (Pubitemid 47254703)
21. Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics. Amend AS, Seifert KA, Samson R, Bruns TD, Proc Natl Acad Sci 2010 107 13748 13753 10.1073/pnas.1000454107 20616017
22. Evaluation of HVAC filters as sampling mechanism for indoor microbial communities. Noris F, Siegel JA, Kinney KA, Atmos Environ 2011 45 338 346 10.1016/j.atmosenv.2010.10.017
23. Analysis of fungal flora in indoor dust by ribosomal DNA sequence analysis, quantitative PCR, and culture. Pitkäranta M, Meklin T, Hyvärinen A, Paulin L, Auvinen P, Nevalainen A, Rintala H, Appl Environ Microbiol 2008 74 233 244 10.1128/AEM.00692-07 17981947
24. The airborne metagenome in an indoor urban environment. Tringe SG, Zhang T, Liu X, Yu Y, Lee WH, Yap J, Yao F, Suan ST, Ing SK, Haynes M, Rohwer F, Wei CL, Tan P, Bristow J, Rubin EM, Ruan Y, PLoS One 2008 3 1862 10.1371/journal.pone.0001862 18382653 (Pubitemid 351954368)
25. Assessment and modeling of indoor fungal and bacterial concentrations. Green CF, Scarpino PV, Gibbs SG, Aerobiologia 2003 19 159 169 (Pubitemid 38094682)
26. The influence of house characteristics in a Canadian community on microbiological contamination. Lawton MD, Dales RE, White J, Indoor Air 1998 8 2 11 10.1111/j.1600-0668.1998.t01-3-00002.x (Pubitemid 128705099)
27. High diversity of fungi in air particulate matter. Fröhlich-Nowoisky J, Pickersgill DA, Despres VR, Poschl U, Proc Natl Acad Sci 2009 106 12814 12819 10.1073/pnas.0811003106 19617562
28. Identification of airborne bacterial and fungal community structures in an urban area by T-RFLP analysis and quantitative real-time PCR. Lee SH, Lee HJ, Kim SJ, Lee HM, Kang H, Kim YP, Sci Total Environ 2010 408 1349 1357 10.1016/j.scitotenv.2009.10.061 19913878
29. Dustborne fungi in large office buildings. Chao HJ, Milton DK, Schwartz J, Burge HA, Mycopathologia 2002 154 93 106 10.1023/A:1015592224368 12086106 (Pubitemid 34604610)
30. Dustborne and airborne fungal propagules represent a different spectrum of fungi with differing relations to home characteristics. Chew GL, Rogers C, Burge HA, Muilenberg ML, Gold DR, Allergy 2003 58 13 20 10.1034/j.1398-9995. 2003.00013.x 12580801 (Pubitemid 36259535)
31. Air-and Dustborne Mycoflora in Houses Free of Water Damage and Fungal Growth. Horner WE, Worthan AG, Morey PR, Appl Environ Microbiol 2004 70 6394 6400 10.1128/AEM.70.11.6394-6400.2004 15528497 (Pubitemid 39518542)
32. Fungal propagules in house dust. I. Comparison of analytic methods and their value as estimators of potential exposure. Verhoeff AP, van Reenen-Hoekstra ES, Samson RA, Brunekreef B, van Wijnen JH, Allergy 1994 49 533 539 10.1111/j.1398-9995.1994.tb01125.x 7825720 (Pubitemid 24287293)
33. Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. von Wintzingerode F, Gobel UB, Stackebrandt E, FEMS Microbiol Rev 1997 21 213 229 10.1111/j.1574-6976.1997.tb00351.x 9451814 (Pubitemid 28018202)
34. Higher Environmental Relative Moldiness Index (ERMIsm) values measured in Detroit homes of severely asthmatic children. Vesper S, McKinstry C, Haugland R, Neas L, Hudgens E, Heidenfelder B, Gallagher J, Sci Total Environ 2008 394 192 196 10.1016/j.scitotenv.2008.01.031 18280542
35. Hydrophilic fungi and ergosterol associated with respiratory illness in a water-damaged building. Park JH, Cox-Ganser JM, Kreiss K, White SK, Rao CY, Environ Health Perspect 2008 116 45 50 18197298
36. Kirk P, Cannon P, Stalpers J, Dictionary of the fungi Wallingford: CABI 10 2008
37. An estimate of the lower limit of global fungal diversity. Schmit JP, Mueller GM, Biodiversity and Conservation 2007 16 99 111 10.1007/s10531-006- 9129-3
38. Can rDNA analyses of diverse fungal communities in soil and roots detect effects of environmental manipulations - a case study from tallgrass prairie. Jumpponen A, Johnson LC, Mycologia 2005 97 1177 1194 10.3852/mycologia.97.6.1177 16722212 (Pubitemid 43738981)
39. Only a few fungal species dominate highly diverse mycofloras associated with the common reed. Neubert K, Mendgen K, Brinkmann H, Wirsel SG, Appl Environ Microbiol 2006 72 1118 1128 10.1128/AEM.72.2.1118-1128.2006 16461657 (Pubitemid 43271217)
40. Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'. Thompson JR, Marcelino LA, Polz MF, Nucleic Acids Res 2002 30 2083 2088 10.1093/nar/30.9.2083 11972349 (Pubitemid 34537607)
41. Fungi and actinobacteria in moisture-damaged building materials - concentrations and diversity. Hyvärinen A, Meklin T, Vepsäläinen A, Nevalainen A, Int Biodeter Biodegr 2002 49 27 37 10.1016/S0964-8305(01)00103- 2 (Pubitemid 34250974)
42. Chapter 2.1 Microbial growth in indoor environments. Flannigan B, Miller JD, Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control Boca Raton: CRC Press, Flannigan B, Samson RA, Miller JD, 2001 35 67
43. Comparison of the indoor air quality in mould damaged and reference buildings in a subarctic climate. Hyvärinen A, Reponen T, Husman T, Nevalainen A, Cent Eur J Public Health 2001 9 133 139 11505735 (Pubitemid 32655934)
44. Qualitative and quantitative PCR methods using species-specific primer for detection and identification of wood rot fungi. Horisawa S, Sakuma Y, Doi S, J Wood Sci 2009 55 133 138 10.1007/s10086-008-1011-3
45. Indoor wood-decay basidiomycetes: damage, causal fungi, physiology, identification and characterization. Schmidt O, Mycol Progress 2007 6 261 279 10.1007/s11557-007-0534-0 (Pubitemid 47632402)
46. Improved molecular methods to characterise Serpula lacrymans and other Basiodiomycetes involved in wood decay. Sundy M, Le Floch G, Le Bras-Quéré M, Barbier G, J Microbiol Methods 2011 84 208 215 10.1016/j.mimet.2010.11.018 21146565
47. de Hoog GS, Guarro J, Gené J, Figueras MJ, Atlas of clinical fungi; a pilot CD-ROM version of the 3rd edition Baarn: Centraalbureau voor Schimmelcultures 2009 21495410
48. Microbial growth and metabolism in house dust. Korpi A, Pasanen A-L, Pasanen P, Kalliokoski P, Int Biodeter Biodegr 1997 40 19 27 10.1016/S0964-8305(97)00032-2 (Pubitemid 28109608)
49. Heteroduplex DNA fingerprinting of Penicillium brevicompactum from house dust. Scott JA, Straus NA, Wong B, Bioaerosols, fungi and mycotoxins: Health effects, assessment, prevention and control Albany: Eastern New York Occupational and Environmental Health Clinic, Johanning E, 1999 335 342
50. Evaluation of a low-cost electrostatic dust fall collector for indoor air endotoxin exposure assessment. Noss I, Wouters IM, Visser M, Heederik DJ, Thorne PS, Brunekreef B, Doekes G, Appl Environ Microbiol 2008 74 5621 7 10.1128/AEM.00619-08 18676704
51. Quantitative PCR analysis of fungi and bacteria in building materials and comparison to culture-based analysis. Pietarinen VM, Rintala H, Hyvärinen A, Lignell U, Kärkkäinen P, Nevalainen A, J Environ Monit 2008 10 655 663 10.1039/b716138g 18449403 (Pubitemid 351624346)
52. Chapter 5: Common and important species of fungi and actinomycetes in indoor environments. Samson RA, Houbraken JS, Summerbell RC, Flannigan B, Miller JD, Microorganisms in home and indoor work environments: diversity, health impacts, investigation and control Boca Raton: CRC Press, Flannigan B, Samson RA, Miller JD, 2001 102 127
53. High-throughput culturing of fungi from plant litter by a dilution-to-extinction technique. Collado J, Platas G, Paulus B, Bills GF, FEMS Microbiol Ecol 2007 60 521 533 10.1111/j.1574-6941.2007.00294.x 17466028 (Pubitemid 46802110)
54. Correlation between ERMI values and other moisture and mold assessments of homes in the American Healthy Homes Survey. Vesper S, McKinstry C, Cox D, Dewalt G, J Urban Health 2009 86 850 860 10.1007/s11524-009-9384-1 19536652
55. Indoor air particles and bioaerosols before and after renovation of moisture-damaged buildings: the effect on biological activity and microbial flora. Huttunen K, Rintala H, Hirvonen MR, Vepsalainen A, Hyvärinen A, Meklin T, Toivola M, Nevalainen A, Environ Res 2008 107 291 298 10.1016/j.envres.2008.02.008 18462714
56. Characterization of the microbial community in indoor environments: a chemical-analytical approach. Sebastian A, Larsson L, Appl Environ Microbiol 2003 69 3103 3109 10.1128/AEM.69.6.3103-3109.2003 12788704 (Pubitemid 36693310)
57. Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis. Haugland RA, Brinkman N, Vesper SJ, J Microbiol Methods 2002 50 319 323 10.1016/S0167-7012(02)00037-4 12031583 (Pubitemid 34603277)
58. EMBL Nucleotide Sequence Database. http://www.ebi.ac.uk/embl
59. PHYLIP (Phylogeny Inference Package) version 3.6. Felsenstein J, Seattle: Department of Genome Sciences, University of Washington 2005 http://www.phylip.com/ Distributed by the author
60. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF, Appl Environ Microbiol 2009 75 7537 41 10.1128/AEM.01541-09 19801464
61. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Huber T, Faulkner G, Hugenholtz P, Bioinformatics 2004 20 2317 2319 http://greengenes.lbl.gov/cgi-bin/nph-bel3-interface.cgi 10.1093/bioinformatics/ bth226 15073015 (Pubitemid 39341624)
62. FigTree. Rambaut A, http://tree.bio.ed.ac.uk/software
63. Identification of moulds in the diagnostic laboratory - an algorithm implementing molecular and phenotypic methods. Ciardo DE, Schar G, Altwegg M, Bottger EC, Bosshard PP, Diagn Microbiol Infect Dis 2007 59 49 60 10.1016/j.diagmicrobio.2007.04.020 17662553 (Pubitemid 47391181)
64. EstimateS: Statistical estimation of species richness and shared species from samples. Colwell RK, http://purl.oclc.org/estimates User's Guide and application
65. R: A language and environment for statistical computing. R Development Core Team, Vienna: R Foundation for Statistical Computing 2008 http://www.R-project.org
66. UniFrac - an online tool for comparing microbial community diversity in a phylogenetic context. Lozupone C, Hamady M, Knight R, BMC Bioinformatics 2006 7 371 http://bmf.colorado.edu/unifrac/ 10.1186/1471-2105-7-371 16893466 (Pubitemid 44390007)