Intron-Specific Neuropeptide Probes

Methods in Molecular Biology

Volumn 789, Issue , 2011, Pages 89-110

  Gainer, Harold ^a   Ponzio, Todd A ^a   Yue, Chunmei ^b   Kawasaki, Makoto ^c  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c UNIVERSITY OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH   (Japan)

Author keywords

Gene expression; Heteronuclear RNA; hn RNA; Oxytocin; Pre mRNA; Real time qPCR; Vasopressin

Indexed keywords

HETEROGENEOUS NUCLEAR RNA; NEUROPEPTIDE; OXYTOCIN; RNA PRECURSOR; VASOPRESSIN DERIVATIVE;

ANIMAL; ARTICLE; GENETICS; HUMAN; IN SITU HYBRIDIZATION; INTRON; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; HUMANS; IN SITU HYBRIDIZATION; INTRONS; NEUROPEPTIDES; OXYTOCIN; REAL-TIME POLYMERASE CHAIN REACTION; RNA PRECURSORS; RNA, HETEROGENEOUS NUCLEAR; VASOPRESSINS;

EID: 80054756674     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-310-3_5     Document Type: Chapter

References (30)

1. Audibert, A., Weil, D., and Dautry, F. (2002) In vivo kinetics of mRNA splicing and transport in mammalian cells. Mol. Cell. Biol. 22: 6706–6718.
2. Clement, J.Q., Qian, L., Kaplinsky, N., and Wilkinson, M.F. (1999) The stability and fate of a spliced intron from vertebrate cells. RNA 5: 206–220.
3. Hargrove, J.L. (1993) Microcomputer-assisted kinetic modeling of mammalian gene expression. FASEB J. 7: 1163–1170.
4. Perry, R.P., Bard, E., Hames, B.D., Kelly, D.E., and Schibler, U. (1976) The relationship between hnRNA and mRNA. Prog. Nucleic Acid Res. Mol. Biol. 19: 275–292.
5. Fremeau, R.T., Jr., Lundblad, J.R., Pritchett, D.B., Wilcox, J.N., and Roberts, J.L. (1986) Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. Science 234: 1265–1269.
6. Fremeau, R.T., Jr., Autelitano, D.J, Blum, M., Wilcox, J., and Roberts, J.L. (1989) Intervening sequence-specific in situ hybridization: detection of the pro-opiomelanocortin gene primary transcript in individual neurons Brain Res. Mol. Brain Re.s 6: 197–201.
7. Chang, M.S., Hahn, M.K., Sved, A.F., Zigmond, M.J., Austin, M.C., and Sherman, T.G. (2000) Analysis of tyrosine hydroxylase gene transcription using an intron specific probe. J. Neurosci. Methods 94: 177–185.
8. Fox, C.A., Mansour, A., Thompson, R.C., Bunzow, J.R., Civelli, O., and Watson, S.J., Jr. (1993) The distribution of dopamine D2 receptor heteronuclear RNA (hnRNA) in the rat brain. J. Chem. Neuroanat. 6: 363–373.
9. Herman, J.P., Schafer, M.K., Watson, S.J., and Sherman, T.G. (1991) In situ hybridization analysis of arginine vasopressin gene transcription using intron-specific probes. Mol. Endocrinol. 5: 1447–1456.
10. Herman, J.P., Schafer, M.K., Thompson, R.C., and Watson, S.J. (1992) Rapid regulation of corticotropin-releasing hormone gene transcription in vivo. Mol. Endocrinol. 6: 1061–1069.
11. Herman, J.P., and Spencer, R. (1998) Regulation of hippocampal glucocorticoid receptor gene transcription and protein expression in vivo. J. Neurosci. 18: 7462–7473.
12. Kovacs, K.J., and Sawchenko, P.E. (1996) Regulation of stress-induced transcriptional changes in the hypothalamic neurosecretory neurons. J. Mol. Neurosci. 7: 125–133.
13. Ma, X.M., Levy, A., and Lightman, S.L. (1997) Rapid changes in heteronuclear RNA for corti-cotrophin-releasing hormone and arginine vasopressin in response to acute stress. J. Endocrinol. 152: 81–89.
14. Paskitti, M.E., McCreary, B.J., and Herman, J.P. (2000) Stress regulation of adrenocorticosteroid receptor gene transcription and mRNA expression in rat hippocampus: time-course analysis. Brain Res. Mol. Brain Res. 80: 142–152.
15. Rusnak, M., and Gainer, H. (2005) Differential effects of forskolin on tyrosine hydroxylase gene transcription in identified brainstem cate-cholaminergic neuronal subtypes in organo-typic culture. Eur. J. Neurosci. 21: 889–898.
16. Brooks, P.J., Kaplitt, M.G., Kleopoulos, S.P., Funabashi, T., Mobbs, C.V., and Pfaff, D.W. (1993) Detection of messenger RNA and low-abundance heteronuclear RNA with single-stranded DNA probes produced by amplified primer extension labeling. J. Histochem. Cytochem. 41: 1761–1766.
17. Douglas, A.J., Meeren, H.K., Johnstone, L.E., Pfaff, D.W., Russell, J.A., and Brooks, P.J. (1998) Stimulation of expression of the oxytocin gene in rat supraoptic neurons at parturition. Brain Res. 782: 167–174.
18. Yue, C., Mutsuga, N., Scordalakes, E. M., and Gainer, H. (2006) Studies of oxytocin and vasopressin gene expression in the rat hypothalamus using exon-and intron-specific probes. Am. J. Physiol. Regul. Integr. Comp. Physiol. 290: R1233–1241.
19. Yue, C, Mutsuga, N, Sugimura, Y, Verbalis, J and Gainer, H. (2008) Differential kinetics of oxytocin and vasopressin heteronuclear RNA expression in the rat supraoptic nucleus in response to chronic salt loading in vivo, J. Neuroendocrinol., 20: 227–232.
20. Yue, C., Ponzio, T. A., Fields, R. L., Gainer, H., (2008) Oxytocin and vasopressin gene expression and RNA splicing patterns in the rat supraoptic nucleus. Physiol. Genomics 35: 231–242.
21. Ponzio, T. A., Yue, C., and Gainer, H.(2007) An intron-based real-time PCR method for measuring vasopressin gene transcription. J. Neurosci. Methods 164: 149–154.
22. Kawasaki, M. Ponzio, T, Yue, C, Fields, RL, and Gainer, H. (2009) Neurotransmitter regulation of c-fos and vasopressin gene expression in the rat supraoptic nucleus. Experimental Neurology 219: 212–222.
23. Young, W.S., 3rd, Mezey, E., and Siegel, R.E. (1986) Vasopressin and oxytocin mRNAs in adrenalectomized and Brattleboro rats: analysis by quantitative in situ hybridization histochemistry. Brain Res. 387: 231–241.
24. Wacker, M.J. and Goddard, M.P. (2005) Analysis of one-step and two-step Real time PCR using superscript III J. Biomolecular Techniques 16: 266–271.
25. Wong, M.L., and Medrano, J.F. (2005) Real time PCR for mRNA quantitation. Biotechniques 39: 75–85.
26. Giegerich, R., Meyer, F., and Schleiermacher, C. (1996) GeneFisher – software support for the detection of postulated genes. Proc. Int. Conf. Intell. Syst. Mol. Biol. 4: 68–77.
27. Livak, K. J., and Schmittgen, T. D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25: 402–408.
28. Pfaffl, M. W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29: e45.
29. Schmittgen, T. D., and Livak, K. J. (2008) Analyzing real-time PCR data by the comparative C(T) method. Nat. Protoc. 3: 1101–1108.
30. Willems, E., Leyns, L., and Vandesompele, J. (2008) Standardization of real-time PCR expression data from independent biological replicates. Anal. Biochem. 379: 127–129.