Diagnosis of ocular toxoplasmosis by two polymerase chain reaction (PCR) examinations: Qualitative multiplex and quantitative real-time

Japanese Journal of Ophthalmology

Volumn 55, Issue 5, 2011, Pages 495-501

  Sugita, Sunao ^a   Ogawa, Manabu ^a   Inoue, Shizu ^a   Shimizu, Norio ^a   Mochizuki, Manabu ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Ocular fluids; Ocular toxoplasmosis; Polymerase chain reaction; Uveitis

Indexed keywords

ACETYLSPIRAMYCIN; CLINDAMYCIN; GANCICLOVIR; GENOMIC DNA; PREDNISOLONE; PROTOZOAL DNA; VALACICLOVIR;

ADULT; AGED; AQUEOUS HUMOR; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS RETINITIS; DNA PROBE; DRUG EFFICACY; FEMALE; GENE AMPLIFICATION; GENE DOSAGE; GENOME ANALYSIS; HUMAN; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RETINA NECROSIS; TOXOPLASMA GONDII; TOXOPLASMOSIS; TREATMENT DURATION; UVEITIS; VITRECTOMY; VITREOUS BODY;

ADULT; AGED; AQUEOUS HUMOR; DNA PRIMERS; DNA PROBES; DNA, PROTOZOAN; DNA, VIRAL; FEMALE; GENOME, PROTOZOAN; HERPESVIRIDAE; HUMANS; MALE; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; TOXOPLASMA; TOXOPLASMOSIS, OCULAR; VITREOUS BODY;

EID: 80054753136     PISSN: 00215155     EISSN: 16132246     Source Type: Journal    
DOI: 10.1007/s10384-011-0065-8     Document Type: Article

References (19)

1. F Aouizerate J Cazenave L Poirier P Verin A Cheyrou J Begueret, et al. 1993 Detection of Toxoplasma gondii in aqueous humour by the polymerase chain reaction Br J Ophthalmol 77 107 109 8435409 10.1136/bjo.77.2.107 1:STN:280:DyaK3s7mvVOguw%3D%3D (Pubitemid 23077679)
2. RM Manners S OConnell EC Guy DH Joynson CR Canning DE Etchells 1994 Use of the polymerase chain reaction in the diagnosis of acquired ocular toxoplasmosis in an immunocompetent adult Br J Ophthalmol 78 583 584 7918275 10.1136/bjo.78.7.583 1:STN:280:DyaK2M%2FgtFOmtg%3D%3D (Pubitemid 24213023)
3. F Robert-Gangneux P Binisti D Antonetti A Brezin H Yera J Dupouy-Camet 2004 Usefulness of immunoblotting and Goldmann-Witmer coefficient for biological diagnosis of toxoplasmic retinochoroiditis Eur J Clin Microbiol Infect Dis 23 34 38 14669070 10.1007/s10096-003-1048-6 1:CAS:528:DC%2BD2cXjtFCksg%3D%3D (Pubitemid 38187020)
4. JD De Groot-Mijnes A Rothova AM Van Loon RA Martinus R Völker NH ten Dam-van Loon, et al. 2006 Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis Am J Ophthalmol 141 313 318 16458686 10.1016/j.ajo.2005.09.017 (Pubitemid 43188032)
5. A Kijlstra L Luyendijk GS Baarsma A Rothova CM Schweitzer Z Timmerman, et al. 1989 Aqueous humor analysis as a diagnostic tool in toxoplasma uveitis Int Ophthalmol 13 383 386 2697703 10.1007/BF02306485 1:STN:280:DyaK3c7os1SltQ%3D%3D (Pubitemid 20098981)
6. R Witmer 1978 Clinical implications of aqueous humor studies in uveitis Am J Ophthalmol 86 39 44 354395 1:STN:280:DyaE1c3jt1yhsw%3D%3D (Pubitemid 8357351)
7. AC Westeneng A Rothova JH de Boer JD de Groot-Mijnes 2007 Infectious uveitis in immunocompromised patients and the diagnostic value of polymerase chain reaction and Goldmann-Witmer coefficient in aqueous analysis Am J Ophthalmol 144 781 785 17707328 10.1016/j.ajo.2007.06.034 (Pubitemid 47614937)
8. K Matos C Muccioli R Belfort Junior LV Rizzo 2007 Correlation between clinical diagnosis and PCR analysis of serum, aqueous, and vitreous samples in patients with inflammatory eye disease Arq Bras Oftalmol 70 109 114 17505729 10.1590/S0004-27492007000100020
9. A Rothova JH de Boer NH Ten Dam-van Loon G Postma L de Visser SJ Zuurveen, et al. 2008 Usefulness of aqueous humor analysis for the diagnosis of posterior uveitis Ophthalmology 115 306 311 17669497 10.1016/j.ophtha.2007.05. 014 (Pubitemid 351163363)
10. JH de Boer C Verhagen M Bruinenberg A Rothova PT de Jong GS Baarsma, et al. 1996 Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis Am J Ophthalmol 121 650 658 8644808 (Pubitemid 26183745)
11. MS Figueroa G Bou P Marti-Belda R Lopez-Velez A Guerrero 2000 Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis Retina 20 614 619 11131414 1:STN:280:DC%2BD3M%2FpslSiug%3D%3D (Pubitemid 32427978)
12. J Bottós RH Miller RN Belfort AC Macedo UNIFESP Toxoplasmosis Group R Belfort Jr, et al. 2009 Bilateral retinochoroiditis caused by an atypical strain of Toxoplasma gondii Br J Ophthalmol 93 1546 1550 19666926 10.1136/bjo.2009.162412
13. MH Lin TC Chen TT Kuo CC Tseng CP Tseng 2000 Real-time PCR for quantitative detection of Toxoplasma gondii J Clin Microbiol 38 4121 4125 11060078 1:CAS:528:DC%2BD3MXhs1Slur0%3D
14. S Patrat-Delon JP Gangneux S Lavoué B Lelong C Guiguen Y le Tulzo, et al. 2010 Correlation of parasite load determined by quantitative PCR to clinical outcome in a heart transplant patient with disseminated toxoplasmosis J Clin Microbiol 48 2541 2545 20463167 10.1128/JCM.00252-10 1:CAS:528: DC%2BC3cXhtVaktbzK
15. A Fekkar B Bodaghi F Touafek P Le Hoang D Mazier L Paris 2008 Comparison of immunoblotting, calculation of the Goldmann-Witmer coefficient, and real-time PCR using aqueous humor samples for diagnosis of ocular toxoplasmosis J Clin Microbiol 46 1965 1967 18400917 10.1128/JCM.01900-07 1:CAS:528: DC%2BD1cXotFGhsL4%3D (Pubitemid 351831828)
16. S Cassaing MH Bessières A Berry A Berrebi R Fabre JF Magnaval 2006 Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR J Clin Microbiol 44 720 724 16517845 10.1128/JCM.44.3.720-724.2006 1:CAS:528:DC%2BD28XjsVyltLw%3D
17. S Sugita N Shimizu K Watanabe M Mizukami T Morio Y Sugamoto, et al. 2008 Use of multiplex PCR and real-time PCR to detect human herpes virus genome in ocular fluids of patients with uveitis Br J Ophthalmol 92 928 932 18408082 10.1136/bjo.2007.133967 1:STN:280:DC%2BD1cvgsFyisg%3D%3D (Pubitemid 351969672)
18. S Sugita Y Iwanaga T Kawaguchi Y Futagami S Horie T Usui, et al. 2008 Detection of herpesvirus genome by multiplex polymerase chain reaction (PCR) and real-time PCR in ocular fluids of patients with acute retinal necrosis Nippon Ganka Gakkai Zasshi 112 30 38 18240601 1:CAS:528:DC%2BD1cXktVKksbY%3D
19. S Sugita N Shimizu K Watanabe M Katayama S Horie M Ogawa, et al. 2011 Diagnosis of bacterial endophthalmitis by broad-range quantitative polymerase chain reaction Br J Ophthalmol 95 345 349 20675730 10.1136/bjo.2009.171504