Bimolecular-fluorescence complementation assay to monitor kinase–substrate interactions in vivo

Methods in Molecular Biology

Volumn 779, Issue , 2011, Pages 245-257

  Pusch, Stefan ^a   Dissmeyer, Nico ^b   Schnittger, Arp ^c  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b LEIBNIZ INSTITUTE OF PLANT BIOCHEMISTRY   (Germany)

^c CNRS   (France)

Author keywords

BiFC; Bimolecular; CDK; Cell cycle; Complementation; Cyclin dependent kinase; Enzyme substrate interaction; Fluorescence; Quantification

Indexed keywords

PROTEIN KINASE; VEGETABLE PROTEIN;

ARTICLE; ENZYME SPECIFICITY; FLUORESCENCE MICROSCOPY; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PHYSIOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM TUMEFACIENS; GENETIC VECTORS; MICROSCOPY, FLUORESCENCE; PLANT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN KINASES; SUBSTRATE SPECIFICITY;

EID: 80054752004     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-264-9_14     Document Type: Chapter

References (27)

1. Fu, H. (2004) Protein-protein interactions: methods and applications, Vol. 261, Humana Press, Totowa, N.J.
2. Werther, M., and Seitz, H. (2008) Protein– Protein Interaction, Vol. 110, Springer-Verlag Berlin Heidelberg, Berlin, Heidelberg.
3. Templin, M. F., Stoll, D., Schrenk, M., Traub, P. C., Vohringer, C. F., and Joos, T. O. (2002) Protein microarray technology. Trends Biotechnol 20, 160–6.
4. Zhu, H., and Snyder, M. (2003) Protein chip technology. Curr Opin Chem Biol 7, 55–63.
5. Yudushkin, I. A., Schleifenbaum, A., Kinkhabwala, A., Neel, B. G., Schultz, C., and Bastiaens, P. I. (2007) Live-cell imaging of enzyme-substrate interaction reveals spatial regulation of PTP1B. Science 315, 115–9.
6. Fields, S., and Song, O. (1989) A novel genetic system to detect protein-protein interactions. Nature 340, 245–6.
7. Causier, B., and Davies, B. (2002) Analysing protein-protein interactions with the yeast two-hybrid system. Plant Mol Biol 50, 855–70.
8. Johnsson, N., and Varshavsky, A. (1994) Split ubiquitin as a sensor of protein interactions in vivo. Proc Natl Acad Sci USA 91, 10340–4.
9. Wittke, S., Lewke, N., Muller, S., and Johnsson, N. (1999) Probing the molecular environment of membrane proteins in vivo. Mol Biol Cell 10, 2519–30.
10. Boeke, J. D., LaCroute, F., and Fink, G. R. (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197, 345–6.
11. Hu, C. D., Chinenov, Y., and Kerppola, T. K. (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9, 789–98.
12. Walter, M., Chaban, C., Schutze, K., Batistic, O., Weckermann, K., Nake, C., Blazevic, D., Grefen, C., Schumacher, K., Oecking, C., Harter, K., and Kudla, J. (2004) Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J 40, 428–38.
13. Bracha-Drori, K., Shichrur, K., Katz, A., Oliva, M., Angelovici, R., Yalovsky, S., and Ohad, N. (2004) Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. Plant J 40, 419–27.
14. Hu, C. D., and Kerppola, T. K. (2003) Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol 21, 539–45.
15. Shyu, Y. J., Suarez, C. D., and Hu, C. D. (2008) Visualization of ternary complexes in living cells by using a BiFC-based FRET assay. Nat Protoc 3, 1693–702.
16. Schütze, K., Harter, K., and Chaban, C. (2009) Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells. Methods Mol Biol 479, 189–202.
17. Tsuchisaka, A., and Theologis, A. (2004) Heterodimeric interactions among the 1-ami-no-cyclopropane-1-carboxylate synthase poly-peptides encoded by the Arabidopsis gene family. Proc Natl Acad Sci USA 101, 2275–80.
18. Tsuchisaka, A., and Theologis, A. (2004) Unique and overlapping expression patterns among the Arabidopsis 1-amino-cyclopropane-1-carboxylate synthase gene family members. Plant Physiol 136, 2982–3000.
19. Bhat, R. A., Lahaye, T., and Panstruga, R. (2006) The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods. Plant Methods 2, 12.
20. Ubersax, J. A., Woodbury, E. L., Quang, P. N., Paraz, M., Blethrow, J. D., Shah, K., Shokat, K. M., and Morgan, D. O. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425, 859–64.
21. Dissmeyer, N., Nowack, M. K., Pusch, S., Stals, H., Inze, D., Grini, P. E., and Schnittger, A. (2007) T-loop phosphorylation of Arabidopsis CDKA;1 is required for its function and can be partially substituted by an aspartate residue. Plant Cell 19, 972–85.
22. Dissmeyer, N., Weimer, A. K., Pusch, S., De Schutter, K., Kamei, C. L., Nowack, M. K., Novak, B., Duan, G. L., Zhu, Y. G., De Veylder, L., and Schnittger, A. (2009) Control of cell proliferation, organ growth, and DNA damage response operate independently of dephosphorylation of the Arabidopsis Cdk1 homolog CDKA;1. Plant Cell 21, 3641–54.
23. Welburn, J. P., Tucker, J. A., Johnson, T., Lindert, L., Morgan, M., Willis, A., Noble, M. E., and Endicott, J. A. (2007) How tyrosine 15 phosphorylation inhibits the activity of cyclin-dependent kinase 2-cyclin A. J Biol Chem 282, 3173–81.
24. Jakoby, M. J., Weinl, C., Pusch, S., Kuijt, S. J., Merkle, T., Dissmeyer, N., and Schnittger, A. (2006) Analysis of the Subcellular Localization, Function, and Proteolytic Control of the Arabidopsis Cyclin-Dependent Kinase Inhibitor ICK1/KRP1. Plant Physiol 141, 1293–305.
25. Feys, B. J., Wiermer, M., Bhat, R. A., Moisan, L. J., Medina-Escobar, N., Neu, C., Cabral, A., and Parker, J. E. (2005) Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity. Plant Cell 17, 2601–13.
26. Koncz, C., and Schell, J. (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204, 383–396.
27. Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33, 949–56.