Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF

Methods in Molecular Biology

Volumn 780, Issue , 2011, Pages 293-312

  Farrar, Dawn ^a   Chernukhin, Igor ^a   Klenova, Elena ^a  

^a UNIVERSITY OF ESSEX   (United Kingdom)

Author keywords

Chromatin immunoprecipitation; Confocal immunofluorescence; CTCF; PCR; Poly(ADP ribosyl)ation; Quantitative PCR; Site directed mutagenesis; Western blot analysis

Indexed keywords

CCCTC-BINDING FACTOR; POLY(ADENOSINE DIPHOSPHATE RIBOSE); REPRESSOR PROTEIN; TRANSCRIPTION FACTOR CTCF;

ANIMAL; ARTICLE; CELL LINE; CHEMISTRY; CHROMATIN IMMUNOPRECIPITATION; GENETICS; HUMAN; METABOLISM; MOUSE; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RAT; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CHROMATIN IMMUNOPRECIPITATION; HUMANS; MICE; MUTAGENESIS, SITE-DIRECTED; POLY ADENOSINE DIPHOSPHATE RIBOSE; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RATS; REPRESSOR PROTEINS;

EID: 80054743488     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-270-0_18     Document Type: Chapter

References (16)

1. Hassa PO, Haenni SS, Elser M et al (2006) Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going? Microbiol Mol Biol Rev 70: 789–829
2. Kanai M, Hanashiro K, Kim SH et al (2007) Inhibition of Crm1-p53 interaction and nuclear export of p53 by poly(ADP-ribosyl)ation. Nat Cell Biol 9:1175–1183
3. Farrar D, Rai S, Chernukhin I et al (2010) Mutational analysis of the poly(ADP-ribosyl) ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation. Mol Cell Biol 30:1199–1216
4. Ohlsson R, Renkawitz R, Lobanenkov V (2001) CTCF is a uniquely versatile transcription regulator linked to epigenetics and disease. Trends Genet 17:520–527
5. Klenova EM, Morse HC 3rd, Ohlsson R et al (2002) The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. Semin Cancer Biol 12:399–414
6. Moon H, Filippova G, Loukinov D et al (2005) CTCF is conserved from Drosophila to humans and confers enhancer blocking of the Fab-8 insulator. EMBO Rep 6:165–170
7. Filippova GN (2008) Genetics and epigenetics of the multifunctional protein CTCF Curr Top Dev Biol 80:337–360
8. Recillas-Targa F, De La Rosa-Velazquez IA, Soto-Reyes E et al (2006) Epigenetic boundaries of tumour suppressor gene promoters: the CTCF connection and its role in carcinogenesis. J Cell Mol Med 10:554–568
9. Ohlsson R, Lobanenkov V, Klenova E (2010) Does CTCF mediate between nuclear organization and gene expression? Bioessays 32:37–50
10. Wallace JA, Felsenfeld G (2007) We gather together: insulators and genome organization. Curr Opin Genet Dev 17:400–407
11. Yu W, Ginjala V, Pant V et al (2004) Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation. Nat Genet 36:1105–1110
12. Caiafa P, Zlatanova J (2009) CCCTC-binding factor meets poly(ADP-ribose) polymerase-1. J Cell Physiol 219: 265–270
13. Torrano V, Navascues J, Docquier F et al (2006) Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism. J Cell Sci 119:1746–1759
14. Witcher M, Emerson BM (2009) Epigenetic silencing of the p16(INK4a) tumor suppressor is associated with loss of CTCF binding and a chromatin boundary. Mol Cell 34:271–284
15. Docquier F, Kita GX, Farrar D et al (2009) Decreased poly(ADP-Ribosyl)ation of CTCF, a transcription factor. Is associated with breast cancer phenotype and cell proliferation. Clin Cancer Res 15:5762–5771
16. Filippova GN, Fagerlie S, Klenova EM et al (1996) An exceptionally conserved transcriptional repressor. CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes. Mol Cell Biol 16:2802–2813