Multiphoton intravital microscopy to study lymphocyte motility in lymph nodes

Methods in Molecular Biology

Volumn 757, Issue , 2011, Pages 247-257

  Murooka, Thomas T ^a   Mempel, Thorsten R ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Confinement ratio; In vivo imaging; Instantaneous velocity; Lymphocyte motility; Mean displacement; Migration; Multiphoton intravital microscopy; Popliteal lymph node; Turning angles

Indexed keywords

ANIMAL; ARTICLE; C57BL MOUSE; CELL MOTION; IMAGE PROCESSING; IMMUNOLOGY; INSTRUMENTATION; LYMPH NODE; LYMPHOCYTE; METHODOLOGY; MOUSE; MULTIPHOTON MICROSCOPY;

ANIMALS; CELL MOVEMENT; IMAGE PROCESSING, COMPUTER-ASSISTED; LYMPH NODES; LYMPHOCYTES; MICE; MICE, INBRED C57BL; MICROSCOPY, FLUORESCENCE, MULTIPHOTON;

MUS;

EID: 80054735407     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-166-6_16     Document Type: Article

References (7)

1. Denk, W., Strickler, J.H., Webb., W.W. (1990) Two-photon laser scanning fluorescence microscopy. Science 248, 73-76.
2. Helmchen, F., Denk, W. (2005) Deep tissue twophoton microscopy. Nat. Methods 2, 932-940.
3. Cahalan, M.D., Parker, I., Wei, S.H., Miller, M.J. (2002) Two-photon tissue imaging: Seeing the immune system in a fresh light. Nat. Rev. Immunol. 2, 872-880. (Pubitemid 37328709)
4. Pittet, M.J., Mempel, T.R. (2008) Regulation of T-cell migration and effector functions: Insights from in vivo imaging studies. Immunol. Rev. 221, 107-129. (Pubitemid 351230111)
5. Miller, M.J., Wei, S.H., Parker, I., Cahalan, M.D. (2002) Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science 296, 1869-1873. (Pubitemid 34596273)
6. Beltman, J.B., A.F. Maree, and R.J. de Boer. (2009) Analysing immune cell migration. Nat. Rev. Immunol. 9, 789-798.
7. Sumen, C., Mempel, T.R., Mazo, I.B., Von Andrian, U.H. (2004) Intravital microscopy; visualizing immunity in context. Immunity 21, 315-329. (Pubitemid 39208333)