Human Pluripotent Stem Cells: The Development of High-Content Screening Strategies

Methods in Molecular Biology

Volumn 767, Issue , 2011, Pages 283-295

  Sherman, Sean P ^a   Alva, Jackelyn A ^a   Thakore Shah, Kaushali ^a   Pyle, April D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

cell fate; chemical genomics; differentiation; HCS; high content screening; human embryonic stem cells; pluripotent stem cells; small molecules; survival

Indexed keywords

CELL FATE; CELL PROLIFERATION; DISSOCIATION; GENOMICS; HIGH CONTENT SCREENING; HUMAN; HUMAN CELL; HUMAN EMBRYONIC STEM CELL; HUMAN EXPERIMENT; INDUCED PLURIPOTENT STEM CELL; ANIMAL; ARTICLE; CELL CULTURE; CELL SURVIVAL; COMPUTER PROGRAM; CYTOLOGY; EMBRYONIC STEM CELL; HIGH THROUGHPUT SCREENING; METABOLISM; METHODOLOGY; MOUSE; PLURIPOTENT STEM CELL; REPRODUCIBILITY; STAINING; STATISTICS; TIME;

ANIMALS; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; EMBRYONIC STEM CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICE; PLURIPOTENT STEM CELLS; REPRODUCIBILITY OF RESULTS; SOFTWARE; STAINING AND LABELING; STATISTICS AS TOPIC; TIME FACTORS;

EID: 80054733923     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-201-4_21     Document Type: Chapter

References (4)

1. Damoiseaux R, Sherman SP, Alva JA, Peterson C, Pyle AD (2009). Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells. Stem Cells 27, 533–542.
2. Malo N, Hanley JA, Cerquozzi S, Pelletier J, Nadon R (2006). Statistical practice in high-throughput screening data analysis. Nat Biotechnol 24, 167–175.
3. Zhang JH, Chung TD, Oldenburg KR (1999). A high-throughput homogeneous assay for reverse transcriptase using generic reagents and time-resolved fluorescence detection. J Biomol Screen 4, 67.
4. Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ (2008). Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells 26: 1436–1443.