An in vitro model for the growth and analysis of chronic wound MRSA biofilms

Journal of Applied Microbiology

Volumn 111, Issue 5, 2011, Pages 1275-1282

  Agostinho, A M ^a   Hartman, A ^a   Lipp, C ^a   Parker, A E ^a   Stewart, P S ^a   James, G A ^a  

^a MONTANA STATE UNIVERSITY   (United States)

Author keywords

Antimicrobial agents; Biofilms; Chronic wounds; In vitro model; MRSA

Indexed keywords

ANTIMICROBIAL AGENTS; BACTERIA; SILVER;

CHRONIC WOUNDS; CLINICAL ISOLATES; FLOW REACTORS; IN-VITRO MODELS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; POLYCARBONATE FILTER MEMBRANES; SALINE SOLUTIONS; SILVER SULFADIAZINES; SOLUTION CONTROLS; STANDARD DEVIATION;

BIOFILMS;

SULFADIAZINE SILVER;

ABSORPTION; ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOFILM; FILTER; GROWTH; INOCULATION; MEMBRANE; MICROSCOPY; MORPHOLOGY; NUMERICAL MODEL; PHYSIOLOGY;

ARTICLE; BACTERIAL GROWTH; BACTERIUM COLONY; BACTERIUM ISOLATE; BIOFILM; CHRONIC WOUND; CONTROLLED STUDY; IN VITRO STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MODEL; MORPHOLOGY; NONHUMAN; REPRODUCIBILITY; THICKNESS; WOUND CARE;

METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS;

EID: 80054689782     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05138.x     Document Type: Article

References (35)

1. American Diabetes Association (1999) Consensus development conference on diabetic foot wound care. Diabetes Care 22, 1354-1360.
2. Anderl, J.N., Franklin, M.J. and Stewart, P.S. (2000) Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother 44, 1818-1824.
3. de Beer, D., Srinivasan, R. and Stewart, P.S. (1994) Direct measurement of chlorine penetration into biofilms during disinfection. Appl Environ Microbiol 60, 4339-4344.
4. Bjarnsholt, T., Kirketerp-Moller, K., Kristiansen, S., Phipps, R., Nielsen, A.K., Jensen, P.O., Hoiby, N. and Givskov, M. (2007) Silver against Pseudomonas aeruginosa biofilms. APMIS 115, 921-928.
5. Bjarnsholt, T., Kirketerp-Moller, K., Jensen, P.O., Madsen, K.G., Phipps, R., Krogfelt, K., Hoiby, N. and Givskov, M. (2008) Why chronic wounds will not heal: a novel hypothesis. Wound Repair Regen 16, 2-10.
6. Brown, D.L. and Smith, D.J. (1999) Bacterial colonization/infection and the surgical management of pressure ulcers. Ostomy Wound Manage 45, 109S-118S.
7. Buckingham-Meyer, K., Goeres, D.M. and Hamilton, M.A. (2007) Comparative evaluation of biofilm disinfectant efficacy tests. J Microbiol Methods 70, 236-244.
8. Centers for Disease Control and Prevention. Diabetes public health resource. Available at: (accessed 10 March 2011).
9. Ceri, H., Olson, M.E., Stremick, C., Read, R.R., Morck, D. and Buret, A. (1999) The Calgary biofilm device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol 37, 1771-1776.
10. Costerton, J.W., Lewandowski, Z., Caldwell, D.E., Korber, D.R. and Lappin-Scott, H.M. (1995) Microbial biofilms. Annu Rev Microbiol 49, 711-745.
11. Costerton, J.W., Veeh, R., Shirtliff, M., Pasmore, M., Post, C. and Ehrlich, G. (2003) The application of biofilm science to the study and control of chronic bacterial infections. J Clin Invest 112, 1466-1477.
12. Davies, C.E., Wilson, M.J., Hill, K.E., Stephens, P., Hill, M.C., Harding, K.G. and Thomas, D.W. (2001) Use of molecular techniques to study microbial diversity in the skin: chronic wounds reevaluated. Wound Repair Regen 9, 32-340.
13. Demling, R.H. and Waterhouse, B. (2007) The increasing problem of wound bacterial burden and infection in acute and chronic soft-tissue wounds caused by methicillin-resistant Staphylococcus aureus. J Burns Wounds 16, 86-98.
14. Donlan, R.M., Murga, R., Carpenter, J., Brown, E., Besser, R. and Fields, B. (2002) Monochloramine disinfection of biofilm associated Legionella pneumophila in a potable water model system. In Legionella ed. Marre, R. pp. 406-410. Washington, DC: American Society for Microbiology.
15. Dowd, S.E., Sun, Y., Secor, P.R., Rhoads, D.D., Wolcott, B.M., James, G.A. and Wolcott, R.D. (2008) Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol 8, 43-58.
16. Feldsine, P., Abeyta, C. and Andrews, W.H. (2002) AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. J AOAC Int 85, 1187-1200.
17. Herrick, S.E., Sloan, P., McGurk, M., Freak, L., McCollum, C.N. and Ferguson, M.W. (1992) Sequential changes in histologic pattern and extracellular matrix deposition during the healing of chronic venous ulcers. Am J Pathol 141, 1085-1095.
18. Hill, K.E., Malic, S., Mckee, R., Rennison, T., Harding, K.G., Williams, D.W. and Thomas, D.W. (2010) An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities. J Antimicrob Chemother 65, 1195-1206.
19. James, G.A., Swogger, E., Wolcott, R., Pulcini, E., Secor, P., Sestrich, J., Costerton, J.W. and Stewart, P.S. (2008) Biofilms in chronic wounds. Wound Repair Regen 16, 37-44.
20. Kostenko, V., Lyczak, J., Turner, K. and Martinuzzi, R.J. (2010) Impact of silver-containing wound dressings on bacterial biofilm viability and susceptibility to antibiotics during prolonged treatment. Antimicrob Agents Chemother 54, 5120-5131.
21. Mah, T.C. and O'Toole, G.A. (2001) Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 9, 34-39.
22. Martin, P. (1997) Wound healing - aiming for perfect skin regeneration. Science 276, 75-81.
23. Miller, C.C., Miller, M.K., Ghaffari, A. and Kunimoto, B. (1992) Treatment of chronic nonhealing leg ulceration with gaseous nitric oxide: a case study. J Cutan Med Surg 8, 233-238.
24. Mittelman, M.W., Kohring, L.L. and White, D.C. (1992) Multipurpose laminar-flow adhesion cells for the study of bacterial colonization and biofilm formation. Biofouling 6, 39-51.
25. Percival, S.L. and Bowler, P.G. (2004) Biofilms and their potential role in wound healing. Wounds 16, 234-240.
26. Percival, S.L., Bowler, P.G. and Dolman, J. (2007) Antimicrobial activity of silver-containing dressings on wound microorganisms using an in vitro biofilm model. Int Wound J 4, 186-191.
27. Pinheiro, J.C. and Bates, D.M. (2000) Statistics and Computing. New York: Springer.
28. Robson, M.C. (1997) Wound infection: a failure of wound healing caused by an imbalance of bacteria. Surg Clin North Am 77, 637-650.
29. Ruckley, C.V. (1997) Socioeconomic impact of chronic venous insufficiency and leg ulcers. Angiology 48, 67-69.
30. Sun, Y., Dowd, S.E., Smith, E., Rhoads, D.D. and Wolcott, R.D. (2008) In vitro multispecies Lubbock chronic wound biofilm model. Wound Repair Regen 16, 805-831.
31. Tarnuzzer, R.W. and Schultz, G. (1996) Biochemical analysis of acute and chronic wound environments. Wound Repair Regen 4, 321-325.
32. Trengove, N.J., Stacey, M.C., McGechie, D.F. and Mata, S. (1996) Qualitative bacteriology and leg ulcer healing. J Wound Care 5, 277-280.
33. Werthen, M., Henriksson, L., Jensen, P.O., Sternberg, C., Givskov, M. and Bjarnsholt, T. (2010) An in vitro model of bacterial infections in wounds and other soft tissues. APMIS 118, 156-164.
34. Xu, K.D., Stewart, P.S., Xia, F., Huang, C.T. and McFeters, G.A. (1998) Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 64, 4035-4039.
35. Zelver, N., Hamilton, M., Pitts, B., Goeres, D., Walker, D., Sturman, P. and Heersink, J. (1999) Measuring antimicrobial effects on biofilm bacteria: from laboratory to field. Methods Enzymol 310, 608-628.