Protected immobilization of Taq DNA polymerase by active site masking on self-assembled monolayers of ω-functionalized thiols

Analytical Biochemistry

Volumn 419, Issue 2, 2011, Pages 205-210

  Lim, Gumjung ^a   Hwang, Hyun Jin ^b   Kim, Jeong Hee ^a  

^a Kyung Hee University   (South Korea)

^b 405 Jangan Bldg 464 1   (South Korea)

Author keywords

Active site masked immobilization; Self assembled monolayer; Taq DNA polymerase

Indexed keywords

AMIDES; DNA; ENZYME ACTIVITY; SELF ASSEMBLED MONOLAYERS;

ACTIVE SITE; BIOLOGICALLY ACTIVE PROTEINS; DNA POLYMERASE; IMMOBILIZATION METHOD; IMMOBILIZED ENZYME; MIXED MONOLAYERS; MIXED SELF ASSEMBLED MONOLAYERS; ORIENTED IMMOBILIZATIONS;

ENZYME IMMOBILIZATION;

CARBOXYL GROUP; DNA POLYMERASE; SELF ASSEMBLED MONOLAYER; SINGLE STRANDED DNA; THIOL;

ARTICLE; COVALENT BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; REACTION TIME;

EID: 80054074902     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.08.022     Document Type: Article

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