DPO multiplex PCR as an alternative to culture and susceptibility testing to detect Helicobacter pylori and its resistance to clarithromycin

BMC Gastroenterology

Volumn 11, Issue , 2011, Pages

  Lehours, Philippe ^a,b,c   Siffré, Elodie ^a   Mégraud, Francis ^a,b,c  

^a UNIVERSITÉ DE BORDEAUX   (France)

^b HÔPITAL PELLEGRIN   (France)

^c Laboratoire de Bactériologie   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CLARITHROMYCIN; ANTIINFECTIVE AGENT; OLIGONUCLEOTIDE; PRIMER DNA;

ANALYTICAL EQUIPMENT; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; DUAL PRIMING OLIGONUCLEOTIDE MULTIPLEX POLYMERASE CHAIN REACTION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MULTIPLEX POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STOMACH BIOPSY; BIOPSY; GENETICS; METHODOLOGY; MICROBIOLOGY; PATHOLOGY; POINT MUTATION; REAL TIME POLYMERASE CHAIN REACTION; STOMACH;

ANTI-BACTERIAL AGENTS; BIOPSY; CLARITHROMYCIN; DNA PRIMERS; DRUG RESISTANCE, BACTERIAL; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; OLIGONUCLEOTIDES; POINT MUTATION; REAL-TIME POLYMERASE CHAIN REACTION; STOMACH;

EID: 80054065945     PISSN: None     EISSN: 1471230X     Source Type: Journal    
DOI: 10.1186/1471-230X-11-112     Document Type: Article

References (12)

1. Malfertheiner P, Megraud F, O'Morain C, Bazzoli F, El-Omar E, Graham D, Hunt R, Rokkas T, Vakil N, Kuipers EJ. Current concepts in the management of Helicobacter pylori infection: the Maastricht III Consensus Report. Gut 2007, 56(6):772-781. 10.1136/gut.2006.101634, 1954853, 17170018.
2. Occhialini A, Urdaci M, Doucet-Populaire F, Bebear CM, Lamouliatte H, Megraud F. Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes. Antimicrob Agents Chemother 1997, 41(12):2724-2728. 164196, 9420046.
3. Menard A, Santos A, Megraud F, Oleastro M. PCR-restriction fragment length polymorphism can also detect point mutation A2142C in the 23S rRNA gene, associated with Helicobacter pylori resistance to clarithromycin. Antimicrob Agents Chemother 2002, 46(4):1156-1157. 10.1128/AAC.46.4.1156-1157.2002, 127079, 11897613.
4. Agudo S, Alarcon T, Urruzuno P, Martinez MJ, Lopez-Brea M. Detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies of pediatric patients by using a commercially available real-time polymerase chain reaction after NucliSens semiautomated DNA extraction. Diagn Microbiol Infect Dis 2010, 67(3):213-219. 10.1016/j.diagmicrobio.2010.02.021, 20542201.
5. Burucoa C, Garnier M, Silvain C, Fauchere JL. Quadruplex real-time PCR assay using allele-specific scorpion primers for detection of mutations conferring clarithromycin resistance to Helicobacter pylori. J Clin Microbiol 2008, 46(7):2320-2326. 10.1128/JCM.02352-07, 2446943, 18463216.
6. Cambau E, Allerheiligen V, Coulon C, Corbel C, Lascols C, Deforges L, Soussy CJ, Delchier JC, Megraud F. Evaluation of a new test, genotype HelicoDR, for molecular detection of antibiotic resistance in Helicobacter pylori. J Clin Microbiol 2009, 47(11):3600-3607. 10.1128/JCM.00744-09, 2772597, 19759218.
7. Oleastro M, Menard A, Santos A, Lamouliatte H, Monteiro L, Barthelemy P, Megraud F. Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J Clin Microbiol 2003, 41(1):397-402. 10.1128/JCM.41.1.397-402.2003, 149634, 12517879.
8. Chun JY, Kim KJ, Hwang IT, Kim YJ, Lee DH, Lee IK, Kim JK. Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene. Nucleic Acids Res 2007, 35(6):e40. 10.1093/nar/gkm051, 1874606, 17287288.
9. Woo HY, Park DI, Park H, Kim MK, Kim DH, Kim IS, Kim YJ. Dual-priming oligonucleotide-based multiplex PCR for the detection of Helicobacter pylori and determination of clarithromycin resistance with gastric biopsy specimens. Helicobacter 2009, 14(1):22-28. 10.1111/j.1523-5378.2009.00654.x, 19191892.
10. Cho AR, Lee MK. [A comparison analysis on the diagnosis of Helicobacter pylori infection and the detection of clarithromycin resistance according to biopsy sites]. Korean J Lab Med 2010, 30(4):381-387. 10.3343/kjlm.2010.30.4.381, 20805710.
11. Megraud F. A growing demand for Helicobacter pylori culture in the near future?. Ital J Gastroenterol Hepatol 1997, 29(6):574-576.
12. Megraud F, Lehours P. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev 2007, 20(2):280-322. 10.1128/CMR.00033-06, 1865594, 17428887.