The sodium-dependent inorganic phosphate transporter SLC34A1 (NaPi-IIa) is not localized in the mouse brain: A case of tissue-specific antigenic cross-reactivity

Journal of Histochemistry and Cytochemistry

Volumn 59, Issue 9, 2011, Pages 807-812

  Larsson, Max ^a,b   Morland, Cecilie ^a   Poblete Naredo, Irais ^c   Biber, Jürg ^d   Danbolt, Niels Christian ^a   Gundersen, Vidar ^a,e  

^a UNIVERSITY OF OSLO   (Norway)

^b KAROLINSKA INSTITUTE   (Sweden)

^c DEPARTAMENTO DE FÍSICA   (Mexico)

^d UNIVERSITY OF ZURICH   (Switzerland)

^e OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

electron microscopy; immunofluorescence; polyclonal; polyreactive; specificity

Indexed keywords

POLYCLONAL ANTISERUM; SODIUM PHOSPHATE COTRANSPORTER 2A; ANTIGEN; SLC34A1 PROTEIN, MOUSE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BRAIN TISSUE; KIDNEY PARENCHYMA; KNOCKOUT GENE; MOUSE; NONHUMAN; PRESYNAPTIC NERVE; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING; WILD TYPE; ANIMAL; ANTIBODY SPECIFICITY; ANTIGEN ANTIBODY REACTION; BRAIN; CHEMISTRY; CROSS REACTION; CYTOLOGY; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; KIDNEY; MOUSE MUTANT;

MUS;

ANIMALS; ANTIBODY SPECIFICITY; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BLOTTING, WESTERN; BRAIN; CROSS REACTIONS; IMMUNOHISTOCHEMISTRY; KIDNEY; MICE; MICE, KNOCKOUT; ORGAN SPECIFICITY; SODIUM-PHOSPHATE COTRANSPORTER PROTEINS, TYPE IIA;

EID: 80054029153     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/0022155411411713     Document Type: Article

References (12)

1. BeckL,KaraplisAC,AmizukaN,HewsonAS,OzawaH,TenenhouseHS.Targeted inactivation of Npt2 in mice leads to severe renal phosphate wasting, hypercalciuria, and skeletal abnormalities.Proc Natl Acad Sci U S A. 1998;95:5372-5377.
2. BergersenLH,Storm-MathisenJ,Gundersen V.Immunogold quantification of amino acids and proteins in complex subcellular compartments.Nat Protoc. 2008;3:144-152.
3. CusterM,LotscherM,BiberJ,MurerH,Kaissling B.Expression of Na-P(i) cotransport in rat kidney: localization by RT-PCR and immunohistochemistry.Am J Physiol. 1994;266:F767-774.
4. DanboltNC,LehreKP,DehnesY,ChaudhryFA,LevyLM.Localization of transporters using transporter-specific antibodies.Methods Enzymol. 1998;296:388-407.
5. HisanoS,HagaH,LiZ,TatsumiS,MiyamotoKI,TakedaE,Fukui Y.Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaPi-2) in rat brain.Brain Res. 1997;772:149-155.
6. HolmsethS,DehnesY,BjornsenLP,BoullandJL,FurnessDN,BerglesD,DanboltNC.Specificity of antibodies: unexpected cross-reactivity of antibodies directed against the excitatory amino acid transporter 3 (EAAT3).Neuroscience. 2005;136:649-660.
7. HolmsethS,LehreKP,DanboltNC.Specificity controls for immunocytochemistry.Anat Embryol (Berl). 2006;211:257-266.
8. KramerA,KeitelT,WinklerK,StockleinW,HohneW,Schneider-Mergener J.Molecular basis for the binding promiscuity of an anti-p24 (HIV-1) monoclonal antibody.Cell. 1997;91:799-809.
9. MagagninS,WernerA,MarkovichD,SorribasV,StangeG,BiberJ,Murer H.Expression cloning of human and rat renal cortex Na/Pi cotransport.Proc Natl Acad Sci U S A. 1993;90:5979-5983.
10. MouquetH,ScheidJF,ZollerMJ,KrogsgaardM,OttRG,ShukairS,ArtyomovMN,PietzschJ,ConnorsM,PereyraF, et al.Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation.Nature. 2010;467:591-595.
11. MurerH,ForsterI,Biber J.The sodium phosphate cotransporter family SLC34.Pflügers Arch. 2004;447:763-767.
12. SaperCB,SawchenkoPE.Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry.J Comp Neurol. 2003;465:161-163.