Optimization of flowrate for expansion of human embryonic stem cells in perfusion microbioreactors

Biotechnology and Bioengineering

Volumn 108, Issue 12, 2011, Pages 2894-2904

  Titmarsh, Drew ^a   Hidalgo, Alejandro ^a,b   Turner, Jennifer ^a   Wolvetang, Ernst ^a   Cooper White, Justin ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b INSTITUTO TECNOLÓGICO DE COSTA RICA   (Costa Rica)

Author keywords

Expansion; Flowrate; Human embryonic stem cells; Maintenance; Microbioreactor; Perfusion

Indexed keywords

CELL EXPANSION; CELL FATES; CULTURE MEDIUM; GLUCOSE TRANSPORT; HUMAN EMBRYONIC STEM CELLS; LINEAR RANGE; LOW RATES; MEDIUM EXCHANGE; MICRO FLUIDIC SYSTEM; MICROBIOREACTOR; MICROENVIRONMENTS; MICROFLUDIC SYSTEMS; MORPHOLOGICAL CHANGES; NUTRIENT DEPLETION; PERFUSION; PERFUSION CONDITIONS; PERFUSION RATE; PLURIPOTENCY; PLURIPOTENT STEM CELLS; SCREENING SYSTEM; STATIC CULTURE; TIME-AVERAGED;

BIOREACTORS; CELL GROWTH; EXPANSION; GLUCOSE; MAINTENANCE; MORPHOLOGY; STEM CELLS;

CELL CULTURE;

ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; CELL EXPANSION; CELL FATE; CELL STRUCTURE; CULTURE MEDIUM; EMBRYONIC STEM CELL; FLOW RATE; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; MICROFLUIDICS; PLURIPOTENT STEM CELL;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CULTURE MEDIA; EMBRYONIC STEM CELLS; HUMANS; MICROFLUIDICS;

EID: 80054017005     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23260     Document Type: Article

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