Paired patch clamp recordings from motor-neuron and target skeletal muscle in zebrafish

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Wen, Hua ^a   Brehm, Paul ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Acetylcholine receptor; Cholinergic action potential; Electrophysiology; Issue 45; Motor control; Myasthenic syndrome; Neuromuscular; Neuroscience; Patch clamp; Synapse; Zebrafish

Indexed keywords

EID: 80053965159     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2351     Document Type: Article

References (9)

1. Fetcho, J.R., & O'Malley, D. Imaging neuronal networks in behaving animals. Current Opinion in Neurobiology. 7, 832-838 (1997).
2. Fetcho, J.R., & Higashijima S. Optical and genetic approaches toward understanding neuronal circuits in zebrafish. Integr Comp Biol 44, 57-70 (2004).
3. Ibañez-Tallon I., Wen H., Miwa J., Xing J., Aslantas-Tekinay, A, Ono, F., Brehm, P., & Heintz, N. Tethering naturally occurring peptide toxins for cell autonomous modulation of ion channels and receptors in vivo. Neuron 43, 305-311 (2004).
4. Li, W., Ono, F. & Brehm, P. Optical measurements of presynaptic release in mutant zebrafish lacking postsynaptic receptors. J. Neuroscience 23, 10467-10474 (2003).
5. Ono, F., Mandel, G. & Brehm, P. Acetylcholine receptors direct rapsyn clusters to the neuromuscular synapse in zebrafish. J. Neuroscience 24, 475-5481 (2004).
6. Ono, F., Shcherbatko, A., Higashijima, S., Fetcho, J., Mandel, G., & Brehm, P. Paralytic zebrafish lacking ACh receptors fail to localize rapsyn clusters to the synapse. J. Neuroscience, 21, 5439-5448 (2001).
7. Ono, F., Shcherbatko, A., Higashijima, S., Mandel, G., & Brehm, P. The zebrafish motility mutant twitch once reveals new roles for rapsyn in synaptic function. J. Neuroscience 22, 6491-6498 (2002).
8. Wang, M., Wen, H., Brehm, P. Function of neuromuscular synapses in the zebrafish choline-acetyltransferase mutant bajan. J Neurophysiol. 100(4):1995-2004 (2008).
9. Wen, H., & Brehm, P. Paired motor-neuron muscle recordings in zebrafish test the receptor blockade model for shaping synaptic current. J. Neuroscience 25, 8104-8111 (2005).