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85034131056
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note
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The single-letter amino acid code is as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Tyr.
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8
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Cold Spring Harbor Laboratory Press: Cold Spring Harbor, Plainview, NY
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Standard methods of DNA manipulation may be found in Sambrook, J.; Frisch, E. F.; Maniatis, T. Molecular Cloning, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, Plainview, NY, 1989.
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85034133613
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note
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pET28ap was constructed by insertion of an 18-base pair linker between the NcoI and XhoI sites of pET28a (Novagen). Insertion eliminated the XhoI site of pET28a, but created a new XhoI site with flanking translational start and stop signals. This construction encodes minimal N- and C-terminal fusion sequences in polymers 1a and 1b.
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1 integrin that serves as the cell-surface receptor for REDV peptides (Masumoto, A.; Hemler, M. E. J. Cell Biol. 1993, 123, 245).
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