Expansion of human peripheral blood γδ T cells using zoledronate

Journal of Visualized Experiments

Volumn , Issue 55, 2011, Pages

  Kondo, Makoto ^a,b   Izumi, Takamichi ^a,b   Fujieda, Nao ^a,b   Kondo, Atsushi ^a,b   Morishita, Takeharu ^a,b   Matsushita, Hirokazu ^a   Kakimi, Kazuhiro ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Medinetcoltd   (United States)

Author keywords

Immunology; Issue 55; PBMC; Peripheral blood mononuclear cells; Zoledronate; T cell

Indexed keywords

EID: 80053930031     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3182     Document Type: Article

References (13)

1. Bonneville, M., O'Brien, R.L., & Born, W.K. γδ T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 10, 467-478 (2010).
2. Hintz, M. et al. Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human γδ T cells in Escherichia coli. FEBS Lett. 509, 317-322 (2001).
3. Gober, H.J., et al. Human T cell receptor γδ cells recognize endogenous mevalonate metabolites in tumor cells. J Exp Med. 197, 163-168 (2003).
4. Kabelitz, D., Wesch, D., & He, W. Perspectives of gammadelta T cells in tumor immunology. Cancer Res. 67, 5-8 (2007).
5. Roelofs, A.J., et al. Peripheral blood monocytes are responsible for gammadelta T cell activation induced by zoledronic acid through accumulation of IPP/DMAPP. Br J Haematol. 144, 245-250 (2009).
6. Dieli, F., et al. Induction of γδ T-lymphocyte effector functions by bisphosphonate zoledronic acid in cancer patients in vivo. Blood. 102, 2310-2311 (2003).
7. Kondo, M., et al. Zoledronate facilitates large-scale ex vivo expansion of functional γδ T cells from cancer patients for use in adoptive immunotherapy. Cytotherapy. 10, 842-856 (2008).
8. Espinosa, E., et al. Chemical synthesis and biological activity of bromohydrin pyrophosphate, a potent stimulator of human γδ T cells. J Biol Chem. 276, 18337-18344 (2001).
9. Kobayashi, H., et al. Safety profile and anti-tumor effects of adoptive immunotherapy using γδ T cells against advanced renal cell carcinoma: a pilot study. Cancer Immunol Immunother. 56, 469-476 (2007).
10. Murali-Krishna, K., et al. Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection. Immunity. 8, 177-187 (1998).
11. Sato, K., et al. Impact of culture medium on the expansion of T cells for immunotherapy. Cytotherapy. 11, 936-946 (2009).
12. Abe, Y., et al. Clinical and immunological evaluation of zoledronate-activated Vγ9 γδT-cell-based immunotherapy for patients with multiple myeloma. Exp Hematol. 37, 956-968 (2009).
13. Nakajima, J., et al. A phase I study of adoptive immunotherapy for recurrent non-small-cell lung cancer patients with autologous γδ T cells. Eur J Cardiothorac Surg. 37, 1191-1197 (2010).