Attenuation of Slc27a5 gene expression followed by LC-MS measurement of bile acid reconjugation using metabolomics and a stable isotope tracer strategy

Journal of Proteome Research

Volumn 10, Issue 10, 2011, Pages 4683-4691

  Castro Perez, Jose M ^a,c   Roddy, Thomas P ^a   Shah, Vinit ^a   Wang, Sheng Ping ^a   Ouyang, Xuesong ^a   Ogawa, Anthony ^a   McLaren, David G ^a   Tadin Strapps, Marija ^b   Robinson, Michael J ^b   Bartz, Steven R ^b   Ason, Brandon ^b   Chen, Ying ^a   Previs, Stephen F ^a   Wong, Kenny K ^a   Vreeken, Rob J ^c   Johns, Douglas G ^a   Hubbard, Brian K ^a   Hankemeier, Thomas ^c   Mitnaul, Lyndon ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b Sirna Therapeutics Inc   (United States)

^c LEIDEN UNIVERSITY   (Netherlands)

Author keywords

accurate mass; bile acids; FATP5; mass spectrometry; mRNA; Slc27a5; stable isotope; tracers; UPLC

Indexed keywords

CARRIER PROTEIN; CHENODEOXYCHOLIC ACID; CHOLIC ACID; DEHYDROCHOLIC ACID; FATTY ACID TRANSPORT PROTEIN 5; GLYCOCHENODEOXYCHOLIC ACID; GLYCOCHOLIC ACID; LITHOCHOLIC ACID; STABLE ISOTOPE; TAUROCHENODEOXYCHOLIC ACID; TAUROCHOLIC ACID; UNCLASSIFIED DRUG; URSODEOXYCHOLIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BILE ACID METABOLISM; BIOLOGICAL MONITORING; CHEMICAL STRUCTURE; CONJUGATION; CONTROLLED STUDY; ENZYME SUBSTRATE; FEMALE; GENE EXPRESSION; GENE SILENCING; IN VITRO STUDY; IN VIVO STUDY; LIQUID CHROMATOGRAPHY; LIVER CELL; MASS SPECTROMETRY; METABOLOMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ANIMALS; BILE ACIDS AND SALTS; CHROMATOGRAPHY, LIQUID; FATTY ACID TRANSPORT PROTEINS; GENE SILENCING; HUMANS; LIVER; MASS SPECTROMETRY; METABOLOMICS; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

MUS;

EID: 80053912889     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr200475g     Document Type: Article

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