Utility of real-time polymerase chain reaction in diagnosing and treating Acanthamoeba keratitis

Cornea

Volumn 30, Issue 11, 2011, Pages 1233-1237

  Itahashi, Motoki ^a   Higaki, Shiro ^b   Fukuda, Masahiko ^b   Mishima, Hiroshi ^a   Shimomura, Yoshikazu ^b  

^a KINDAI UNIVERSITY NARA HOSPITAL   (Japan)

^b KINKI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Acanthamoeba; culture; detection and quantification of pathogens; keratitis; real time PCR

Indexed keywords

CHLORHEXIDINE; FLUCONAZOLE; LEVOFLOXACIN; MICONAZOLE; PROTOZOAL DNA;

ACANTHAMOEBA; ACANTHAMOEBA KERATITIS; ADULT; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CLINICAL ARTICLE; COPY NUMBER VARIATION; CORNEA EDEMA; CORNEA OPACITY; CORNEAL BIOPSY; CORYNEBACTERIUM; DISEASE SEVERITY; EYE PAIN; FEMALE; GENE DOSAGE; HUMAN; HUMAN TISSUE; MALE; NONHUMAN; PATIENT MONITORING; PRIORITY JOURNAL; PUNCTATE KERATITIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SOFT CONTACT LENS; TOPICAL TREATMENT; TREATMENT DURATION;

ACANTHAMOEBA; ACANTHAMOEBA KERATITIS; ADULT; ANTIPROTOZOAL AGENTS; CHLORHEXIDINE; CONTACT LENSES, HYDROPHILIC; CORNEA; DNA COPY NUMBER VARIATIONS; DNA, PROTOZOAN; FEMALE; HUMANS; MALE; MICONAZOLE; OPHTHALMIC SOLUTIONS; REAL-TIME POLYMERASE CHAIN REACTION; YOUNG ADULT;

EID: 80053649511     PISSN: 02773740     EISSN: 15364798     Source Type: Journal    
DOI: 10.1097/ICO.0b013e3182032196     Document Type: Article

References (18)

1. Patel DV, McGhee CN. Acanthamoeba keratitis: A comprehensive photographic reference of common and uncommon signs. Clin Experiment Ophthalmol. 2009;37:232-238.
2. Centers for Disease Control and Prevention (CDC). Acanthamoeba keratitis multiple states, 2005-2007. MMWR Morb Mortal Wkly Rep. 2007;56:532-534.
3. Shiraishi A, Kobayashi T, Hara Y, et al. Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y. Br J Ophthalmol. 2009;93:1555-1556.
4. Inoue T, Asari S, Tahara K, et al. Utility of Fungiflora Y stain in rapid diagnosis of Acanthamoeba keratitis. Br J Ophthalmol. 1999;83:632-633. (Pubitemid 29213149)
5. Hahn TW, O'Brien TP, Sah WJ, et al. Acridine orange staining for rapid diagnosis of Acanthamoeba keratitis. Jpn J Ophthalmol. 1998;42: 108-114. (Pubitemid 28254698)
6. Lehmann OJ, Green SM, Morlet N, et al. Polymerase chain reaction analysis of corneal epithelial and tear samples in the diagnosis of Acanthamoeba keratitis. Invest Ophthalmol Vis Sci. 1998;39:1261-1265. (Pubitemid 28247363)
7. Mathers WD, Nelson SE, Lane JL, et al. Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol. 2000;118:178-183. (Pubitemid 30086203)
8. Schroeder JM, Booton GC, Hay J, et al. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. J Clin Microbiol. 2001;39:1903-1911. (Pubitemid 32417041)
9. Yera H, Zamfir O, Bourcier T, et al. Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections. Eur J Clin Microbiol Infect Dis. 2007;26:221-224. (Pubitemid 46723493)
10. Nakano E, Oliveira M, Portellinha W, et al. Confocal microscopy in early diagnosis of Acanthamoeba keratitis. J Refract Surg. 2004;20:737-740.
11. Riviére D, Szczebara FM, Berjeaud JM, et al. Development of a real-time PCR assay for quantification of Acanthamoeba trophozoites and cysts. J Microbiol Methods. 2006;64:78-83. (Pubitemid 41790488)
12. Qvarnstrom Y, Visvesvara GS, Sriram R, et al. Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. J Clin Microbiol. 2006;44: 3589-3595. (Pubitemid 46768828)
13. Thompson PP, Kowalski RP, Shanks RM, et al. Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis. J Clin Microbiol. 2008;46:3232-3236.
14. Kandori M, Inoue T, Takamatsu F, et al. Two cases of Acanthamoeba keratitis diagnosed only by real-time polymerase chain reaction. Cornea. 2010;29:228-231.
15. Itahashi M, Higaki S, Fukuda M, et al. Detection and quantification of pathogenic bacteria and fungi using real-time polymerase chain reaction by cycling probe in patients with corneal ulcer. Arch Ophthalmol. 2010; 128:535-540.
16. Joslin CE, Tu EY, Shoff ME, et al. The association of contact lens solution use and Acanthamoeba keratitis. Am J Ophthalmol. 2007;144: 169-180.
17. Ku JY, Chan FM, Beckingsale P. Acanthamoeba keratitis cluster: An increase in Acanthamoeba keratitis in Australia. Clin Experiment Ophthalmol. 2009;37:181-190.
18. Tu EY, Joslin CE, Sugar J, et al. The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis. Cornea. 2008;27:764-772.