Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 26, Issue 3, 2007, Pages 221-224

  Yera, H ^a   Zamfir, O ^b   Bourcier, T ^b   Ancelle, T ^a   Batellier, L ^b   Dupouy Camet, J ^a   Chaumeil, C ^b  

^a Université Paris Descartes ^*  (France)

^b CENTRE HOSPITALIER NATIONAL D OPHTALMOLOGIE DES QUINZE VINGTS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RIBOSOME RNA;

ACANTHAMOEBA; ACANTHAMOEBA KERATITIS; ARTICLE; CONTROLLED STUDY; CORNEA; DNA SEQUENCE; EARLY DIAGNOSIS; GENE; GENOTYPE; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MICROSCOPY; NUCLEOTIDE SEQUENCE; PARASITE ISOLATION; PARASITOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RISK FACTOR; SAMPLING; VIRUS CHARACTERIZATION; VIRUS CULTURE;

EID: 34248153801     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-007-0268-6     Document Type: Article

References (10)

1. Stothard DR, Hay J, Schroeder-Diedrich JM, Seal DV, Byers TJ (1999) Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. J Clin Microbiol 37:2687-2693
2. Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R (2000) Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 118:178-183
3. Lehmann OJ, Green SM, Morlet N, Kilvington S, Keys MF, Matheson MM, Dart JK, McGill JI, Watt PJ (1998) Polymerase chain reaction analysis of corneal epithelial and tear samples in the diagnosis of Acanthamoeba keratitis. Invest Ophthalmol Vis Sci 39:1261-1265
4. Pasricha G, Sharma S, Garg P, Aggarwal RK (2003) Use of 18S rRNA gene-based PCR assay for diagnosis of Acanthamoeba keratitis in non-contact lens wearers in India. J Clin Microbiol 41:3206-3211
5. Schroeder JM, Booton GC, Hay J, Niszl IA, Seal DV, Markus MB, Fuerst PA, Byers TJ (2001) Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoeba from humans with keratitis and from sewage sludge. J Clin Microbiol 39:1903-1911
6. Booton GC, Kelly DJ, Chu YW, Seal DV, Houang E, Lam DS, Byers TJ, Fuerst PA (2002) 18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lenses, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong. J Clin Microbiol 40:1621-1625
7. Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, Dean CL, Fuerst PA, Byers TJ (1998) The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukaryot Microbiol 45:45-54
8. Walochnik J, Obwaller A, Aspock H (2000) Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl Environ Microbiol 66:4408-4413
9. Booton GC, Visvesvara GS, Byers TJ, Kelly DJ, Fuerst PA (2005) Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. J Clin Microbiol 43:1689-1693
10. Visvesvara GS (1991) Classification of Acanthamoeba. Rev Infect Dis 13(Suppl 5):369-372