Biotransformation enzyme-dependent formation of micronucleus and multinuclei in cell line V79-hCYP2E1-hSULT1A1 by 2-nitropropane and N-nitrosodimethylamine

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 726, Issue 1, 2011, Pages 84-87

  Deng, Hong ^a   Gao, Hai ^a   Liu, Yungang ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

2 Nitropropane; CYP2E1; Micronucleus; Multinuclei; N nitrosodimethylamine; Sulfotransferase (SULT)

Indexed keywords

2 NITROPROPANE; CYTOCHROME P450 2E1; DIMETHYLNITROSAMINE; SULFOTRANSFERASE 1A1;

ARTICLE; BIOTRANSFORMATION; CELL LINE; CELL STRAIN V 79; CYTOTOXICITY; GENOTOXICITY; HUMAN; METABOLITE; MICRONUCLEUS; MULTINUCLEAR CELL; MUTAGENIC ACTIVITY; PRIORITY JOURNAL; V79 HCYP2E1 HSULT1A1 CELL LINE;

ARYLSULFOTRANSFERASE; BIOTRANSFORMATION; CELL LINE; CYTOCHROME P-450 CYP2E1; DIMETHYLNITROSAMINE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MUTAGENS; NITROPARAFFINS; PROPANE;

ANIMALIA;

EID: 80053649309     PISSN: 13835718     EISSN: 18793592     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2011.08.001     Document Type: Article

References (29)

1. Lewis T.R., Ulrich C.E., Busey W.M. Subchronic inhalation toxicity of nitromethane and 2-nitropropane. J. Environ. Pathol. Toxicol. 1979, 2:233-249.
2. Fiala E.S., Czerniak R., Castonguay A., Conaway C.C., Rivenson A. Assay of 1-nitropropane, 2-nitropropane, 1-azoxypropane and 2-azoxypropane for carcinogenicity by gavage in Sprague-Dawley rats. Carcinogenesis 1987, 8:1947-1949.
3. Andrae U., Homfeldt H., Vogl L., Lichtmannegger J., Summer K.H. 2-Nitropropane induces DNA repair synthesis in rat hepatocytes in vitro and in vivo. Carcinogenesis 1988, 9:811-815.
4. George E., Burlinson B., Gatehouse D. Genotoxicity of 1- and 2-nitropropane in the rat. Carcinogenesis 1989, 10:2329-2334.
5. Goggelmann W., Bauchinger M., Kullka U., Schmid E. Genotoxicity of 2-nitropropane and 1-nitropropane in Salmonella typhimurium and human lymphocytes. Mutagenesis 1988, 3:137-140.
6. Andrae U., Kreis P., Coughtrie M.W.H., Pabel U., Meinl W., Bartsch I., Glatt H.R. Activation of propane 2-nitronate to a genotoxicant in V79-derived cell lines engineered for the expression of rat hepatic sulfotransferases. Mutat. Res. 1999, 439:191-197.
7. Kreis P., Brandner S., Coughtrie M.W.H., Pabel U., Meinl W., Glatt H.R., Andrae U. Human phenol sulfotransferases hP-PST and hM-PST activate propane 2-nitronate to a genotoxicant. Carcinogenesis 2000, 21:295-299.
8. Liu Y.G., Glatt H.R. Mutagenicity of N-nitrosodiethanolamine in a V79-derived cell line expressing two human biotransformation enzymes. Mutat. Res. 2008, 643:64-69.
9. Sodum R.S., Nie G., Fiala E.S. 8-Aminoguanine: a base modification produced in rat liver nucleic acids by the hepatocarcinogen 2-nitropropane. Chem. Res. Toxicol. 1993, 6:269-276.
10. Sodum R.S., Sohn O.S., Nie G., Fiala E.S. Activation of the liver carcinogen 2-nitropropane by aryl sulfotransferase. Chem. Res. Toxicol. 1994, 7:344-351.
11. Smith C.J., Perfetti T.A., Rumple M.A., Rodgman A., Doolittle D.J. " IARC Group 2 carcinogens" reported in cigarrete mainstream smoke. Food Chem. Toxicol. 2000, 38:371-383.
12. Chen W.H., Young T.M. Influence of nitrogen source on NDMA formation during chlorination of diuron. Water Res. 2009, 43:3047-3056.
13. Peto R., Gray R., Brantom P., Grasso P. Effects on 4080 rats of chronic administration of N-nitrosodiethylamine or N-nitrosodimethylamine. A detailed dose response study. Cancer Res. 1991, 51:6451-6515.
14. Schmalix W.A., Barrenscheen M., Landsiedel R., Janzowski C., Eisenbrand G., Gonzalez F., Eliasson E., Ingelman-Sundberg M., Perchermeier M., Greim H., Doehmer J. Stable expression of human cytochrome P450 2E1 in V79 Chinese hamster cells. Eur. J. Pharmacol. 1995, 293:123-131.
15. Katic J., Cemeli E., Baumgartner A., Laubenthal J., Bassano I., Stolevik S.B., Granum B., Namork E., Nygaard U.C., Lovik M., van Leeuwen D., Loock K.V., Anderson D., Fucic A., Decordier I. Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison. Food Chem. Toxicol. 2010, 48:2612-2623.
16. Jenssen D., Beije B., Ramel C. Mutagenicity testing on Chinese hamster V79 cells treated in the in vitro liver perfusion system: comparative investigation of different in vitro metabolosing systems with dimethylnitrosamine and benzo(a)pyrene. Chem. Biol. Interact. 1979, 27:27-39.
17. Kroeger-Koepke M.B., Koepke S.R., McClusky G.A., Magee P.N., Michejda C.J. α-Hydroxylation pathway in the in vitro metabolism of carcinogenic nitrosamines: N-nitrosodimethylamine and N-nitroso-N-methylaniline. Proc. Natl. Acad. Sci. U.S.A. 1981, 78:6489-6493.
18. Fournier P. Biotransformation of dimethylnitrosamine. J. Toxicol. Clin. Exp. 1990, 10:283-296.
19. Liu Y.G., Wu Z.L., Chen J.K. Differential effects of aneugens and clastogens on incidences of multinucleated cells and micronucleate cells in Chinese hamster lung (V79) cell line in vitro. Mutat. Res. 1998, 413:39-45.
20. Liu Y.G. Differential effects of three anti-tumor drugs on frequencies of multinucleated cells and of micronucleate cells in human bronchotracheal fibroblastic cells in vitro. Int. J. Modern Cancer Ther. 2000, 3:35-37.
21. Matsuoka A., Matsuura K., Sakamoto H., Hayashi M., Sofuni T. A proposal for a simple way to distinguish aneugens from clastogens in the in vitro micronucleus test. Mutagenesis 1999, 14:385-389.
22. Rodilla V. Origin and evolution of binucleated cells and binucleated cells with micronuclei in cisplatin-treated CHO culture. Mutat. Res. 1993, 300:281-291.
23. Liu Y.G., Glatt H.R. Human SULT1A1 and CYP2E1 co-expressed in Chinese hamster V79 cells enhance spontaneous mutagenesis. Environ. Mol. Mutagen. 2010, 51:23-30.
24. Glatt H.R., Schneider H., Liu Y.G. V79-hCYP2E1-hSULT1A1, a cell line for sensitive detection of genotoxic effects induced by carbohydrate pyrolysis products and other food-borne chemicals. Mutat. Res. 2005, 580:41-52.
25. Meinl W., Pabel U., Osterloh-Quiroz M., Hengster J.G., Glatt H.R. Human sulphotransferases are involved in the activation of aristolochic acids and expressed in renal target tissue. Int. J. Cancer 2006, 118:1090-1097.
26. 1 mice. J. Toxicol. Environ. Health 1995, 44:219-233.
27. Abraham I., Marcus M., Cabral F., Gottesman M.M. Mutations in α- and β-tubulin affect spindle formation in Chinese hamster ovary cells. J. Cell Biol. 1983, 97:1055-1061.
28. Ochi T. Induction of centrosome injury, multipolar spindles and multipolar division in cultured V79 cells exposed to dimethylarsinic acid: role for microtubules in centrosome dynamics. Mutat. Res. 2000, 454:21-33.
29. Shekhar M.P.V., Lyakhovich A., Visscher D.W., Heng H., Kondrat N. Rad6 overexpression induces multinucleation, centrosome amplification, abnormal mitosis, aneuploidy, and transformation. Cancer Res. 2002, 62:2115-2124.