Heat stability of Proteobacterial P II protein facilitate purification using a single chromatography step

Protein Expression and Purification

Volumn 81, Issue 1, 2012, Pages 83-88

  Moure, Vivian R ^a   Razzera, Guilherme ^b   Araújo, Luíza M ^a   Oliveira, Marco A S ^a   Gerhardt, Edileusa C M ^a   Müller Santos, Marcelo ^a   Almeida, Fabio ^b   Pedrosa, Fabio O ^a   Valente, Ana P ^b   Souza, Emanuel M ^a   Huergo, Luciano F ^a  

^a FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Heat treatment; Melting temperature; P II proteins; Protein NMR

Indexed keywords

BACTERIAL PROTEIN; GLNZ PROTEIN, AZOSPIRILLUM BRASILENSE; NITROGEN REGULATORY PROTEIN; PIID REGULATORY PROTEIN, BACTERIA; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; AZOSPIRILLUM BRASILENSE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; TRANSITION TEMPERATURE;

AZOSPIRILLUM BRASILENSE; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PII NITROGEN REGULATORY PROTEINS; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; RECOMBINANT PROTEINS; TRANSITION TEMPERATURE;

AZOSPIRILLUM BRASILENSE; ESCHERICHIA COLI; HERBASPIRILLUM; HERBASPIRILLUM SEROPEDICAE; PROTEOBACTERIA;

EID: 80053620562     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.09.008     Document Type: Article

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