Different responses of the GlnB and GlnZ proteins upon in vitro uridylylation by the Azospirillum brasilense GlnD protein

Brazilian Journal of Medical and Biological Research

Volumn 41, Issue 4, 2008, Pages 289-294

  Araujo L M ^a   Huergo, L F ^a   Invitti, A L ^a   Gimenes, C I ^a   Bonatto, A C ^a   Monteiro, R A ^a   Souza, E M ^a   Pedrosa, F O ^a   Chubatsu, L S ^a  

^a FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

Author keywords

Azospirillum brasilense; GlnB; GlnD; GlnZ; Nitrogen fixation; PII like protein

Indexed keywords

2 OXOGLUTARIC ACID; ADENOSINE TRIPHOSPHATE; GLUTAMINE; HEXOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; NITROGEN; PROTEIN GLNB; PROTEIN GLND; PROTEIN GLNZ;

ARTICLE; AZOSPIRILLUM BRASILENSE; CHEMICAL REACTION; CONTROLLED STUDY; DEURIDYLYLATION; ENZYME RECONSTITUTION; FLUORESCENCE; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION; URIDYLATION;

AZOSPIRILLUM BRASILENSE;

EID: 44449124037     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2008000400006     Document Type: Article

References (20)

1. Arcondeguy T, Jack R, Merrick M. P(II) signal transduction proteins, pivotal players in microbial nitrogen control. Microbiol Mol Biol Rev 2001; 65: 80-105.
2. Adler SP, Purich D, Stadtman ER. Cascade control of Escherichia coli glutamine synthetase. Properties of the PII regulatory protein and the uridylyltransferase-uridylyl-removing enzyme. J Biol Chem 1975; 250: 6264-6272.
3. Ninfa AJ, Jiang P. PII signal transduction proteins: sensors of alpha-ketoglutarate that regulate nitrogen metabolism. Curr Opin Microbiol 2005; 8: 168-173.
4. Commichau FM, Forchhammer K, Stulke J. Regulatory links between carbon and nitrogen metabolism. Curr Opin Microbiol 2006; 9: 167-172,
5. Döbereiner J, Pedrosa FO. Nitrogen-fixing bacteria in nonleguminous crop plants. Madison: Springer-Veriag: Science Tech Publishers; 1987.
6. de Zamaroczy M. Structural homologues P(II) and P(Z) of Azospirillum brasilense provide intracellular signalling for selective regulation of various nitrogen-dependent functions. Mol Microbiol 1998; 29: 449-463.
7. Huergo LF, Souza EM, Steffens MB, Yates MG, Pedrosa FO, Chubatsu LS. Regulation of glnB gene promoter expression in Azospirillurn brasilense by the NtrC protein. FEMS Microbiol Lett 2003; 223: 33-40.
8. Arsene F, Kaminski PA, Elmerich C. Modulation of NifA activity by PH in Azospirillum brasilense: evidence for a regulatory role of the NifA N-terminal domain. J Bacteriol 1996; 178: 4830-4838.
9. Araujo LM, Monteiro RA, Souza EM, Steffens MB, Rigo LU, Pedrosa FO, et al. GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli. Res Microbiol 2004; 155: 491-495.
10. Huergo LF, Chubatsu LS, Souza EM, Pedrosa FO, Steffens MB, Merrick M. Interactions between PH proteins and the nitrogenase regulatory enzymes DraT and DraG in Azospirillum brasilense. FEBS Lett 2006; 580: 5232-5236.
11. Huergo LF, Souza EM, Araujo MS, Pedrosa FO, Chubatsu LS, Steffens MB, et al. ADP-ribosylation of dinitrogenase reductase in Azospirillum brasilense is regulated by AmtB- dependent membrane sequestration of DraG. Mol Microbiol 2006; 59: 326-337.
12. Araujo MS, Baura VA, Souza EM, Benelli EM, Rigo LU, Steffens MB, et al. In vitro uridylylation of the Azospirillum, brasilense N-signal transducing GlnZ protein. Protein Expr Purif 2004; 33: 19-24.
13. Forchhammer K, Hedler A, Strobel H, Weiss V. Heterotrimerization of PII-like signalling proteins: implications for PII-mediated signal transduction systems. Mol Microbiol 1999; 33: 338-349.
14. Son HS, Rhee SG. Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PH protein and nucleotide sequence of its structural gene. J Biol Chem 1987; 262: 8690-8695.
15. Huergo LF, Merrick M, Pedrosa FO, Chubatsu LS, Araujo LM, Souza EM. Ternary complex formation between AmtB, GlnZ and the nitrogenase regulatory enzyme DraG reveals a novel facet of nitrogen regulation in bacteria. Mol Microbiol 2007; 66: 1523-1535.
16. Gruswitz: F, O'Connell J III, Stroud RM, Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A. Proc Natl Acad Sci U S A 2007; 104: 42-47.
17. Conroy MJ, Durand A, Lupo D, Li XD, Bullough PA, Winkler FK, et al. The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel. Proc Natl Acad Sci U S A 2007, 104: 1213-1218.
18. Senior PJ. Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique. J Bacteriol 1975; 123: 407-418.
19. Xu Y, Carr PD, Huber T, Vasudevan SG, Ollis DL. The structure of the PII-ATP complex. Eur J Biochem 2001; 268: 2028-2037.
20. Jiang P, Peliska JA, Ninfa AJ. Enzymological characterization of the signal-transducing uridylyltransferase/uridylyl-removing enzyme (EC 2.7.7.59) of Escherichia coli and its interaction with the PII protein. Biochemistry 1998; 37: 12782-12794.