Synthesis of a multivalent, multiepitope vaccine construct

Methods in Molecular Biology

Volumn 494, Issue , 2008, Pages 263-273

  Otvos Jr , Laszlo ^a  

^a TEMPLE UNIVERSITY   (United States)

Author keywords

Automated peptide synthesis; Influenza virus; Oligolysine scaffold; Orthogonal side chain protection; Protective antibody; T helper cell determinant

Indexed keywords

MUS; ORTHOMYXOVIRIDAE;

EID: 80053604919     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-419-3_15     Document Type: Article

References (24)

1. Jiang, S., and Debnath, A.K. (2000) Development of HIV entry inhibitors targeted to the coiled-coil regions of gp41. Biochem. Biophys. Res. Commun. 269, 641-646.
2. Rojanasakul, Y., Luo, Q., Ye, J., Antonini, J., and Toledo, D. (2000) Cellular delivery of functional peptides to block cytokine gene expression. J. Control. Rel. 65, 13-17.
3. Otvos, L. Jr. (2005) Antibacterial peptides and proteins with multiple cellular targets. J. Pept. Sci. 11, 697-706.
4. Dietzschold, B., Gore, M., Marchandiar, D., et al. (1990) Structural and immunological characterization of a linear virus-neutralizing epitope of the rabies virus glycoprotein and its possible use in a synthetic vaccine. J. Virol. 64, 3804-3809.
5. Hsu, S.C., Chargelegue, D., Obeid, O.E., and Steward, M.W. (1999) Synergistic effect of immunization with a peptide cocktail inducing antibody, helper and cytotoxic T-cell responses on protection against respiratory syncytial virus. J. Gen. Virol. 80, 1401-1405.
6. Meyer, D., and Torres, J.V. (1999) Hypervariable epitope construct: a synthetic immunogen that overcomes MHC restriction of antigen presentation. Mol. Immunol. 36, 631-637.
7. Triozzi, P.L., Stoner, G.D., and Kaumaya, P.T. (1995) Subunit peptide cancer vaccines targeting activating mutations of the p21 ras proto-oncogene. Biomed. Pept. Proteins Nucleic Acids 1, 185-192.
8. Fitzmaurice, C.J., Brown, L.E., Kronin, K., and Jackson, D.C. (2000) The geometry of synthetic peptide-based immunogens affects the efficiency of T cell stimulation by professional antigen-presenting cells. Int. Immunol. 12, 527-535.
9. Cruz, L.J., Quintana, D., Iglesias, E., et al. (2000) Immunogenicity comparison of a multi-antigenic peptide bearing V3 sequences of the human immunodeficiency virus type 1 with TAB9 protein in mice. J. Pept. Sci. 6, 217-224.
10. Tam, J. (1988) Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proc. Natl. Acad. Sci. USA 85, 5409-5413.
11. Wang, J., Kim, S., Kovacs, F., and Cross, T.A. (2001) Structure of the transmembrane region of the M2 protein H(+) channel. Protein Sci. 10, 2241-2250.
12. Shih, F.F., Cerasoli, D.M., and Caton, A.J. (1997) A major T cell determinant from the influenza virus hemagglutinin (HA) can be a cryptic self peptide in HA transgenic mice. Int. Immunol. 9, 249-261.
13. Kragol, G., Otvos, L., Jr., Feng, J., Gerhard, W., and Wade, J.D. (2001) Synthesis of a disulfide-linked octameric peptide construct carrying three different antigenic determinants. Bioorg. Med. Chem. Lett. 11, 1417-1420.
14. Mozdzanowska, K., Feng, J., Eid, M., et al. (2003) Induction of influenza type A virus-specific resistance by immunization of mice with a synthetic multiple antigenic peptide vaccine that contains ectodomains of matrix protein 2. Vaccine 21, 2616-2626.
15. Zhang, M., Zharikova, D., Mozdzanowska, K., Otvos, L., Jr., and Gerhard, W. (2006) Fine specificity and sequence of antibodies directed against the ectodomain of matrix protein 2 of influenza A virus. Mol. Immunol. 43, 2195-2206.
16. Zharikova, D., Mozdzanowska, K., Feng, J., Zhang, M., and Gerhard, W. (2005) Influenza type A virus escape mutants emerge in vivo in the presence of antibodies to the ectodomain of matrix protein 2. J. Virol. 79, 6644-6654.
17. Pawlak, M., Meseth, U., Dhanapal. B., Mutter, M., and Vogel, H. (1994) Template-assembled melittin: Structural and functional characterization of a designed, synthetic channel-forming protein. Protein Sci. 3, 1788-1805.
18. Wade, J.D., Beford, J., Sheppard, R.C., and Tregear, G.W. (1991) DBU as an N-alpha-deprotecting reagent for the fluorenylmethoxycarbonyl group in continuous flow solid-phase peptide synthesis. Pept. Res. 4, 194-199.
19. Kragol, G., and Otvos, L., Jr. (2001) Orthogonal solid-phase synthesis of tetramannosylated peptide constructs carrying three independent branched epitopes. Tetrahedron 57, 957-966.
20. Palasek, S.A., Cox, Z.J., and Collins, J.M. (2007) Limiting racemization and aspartimide formation in microwave-enhanced Fmoc solid phase peptide synthesis. J. Pept. Sci. 13, 143-148.
21. Angell, Y.M., Garcia-Echeverria, C., and Rich, D.H. (1994) Comparative studies of the coupling of N-methylated sterically hindered amino acids during solid-phase peptide synthesis. Tetrahedron Lett. 35, 5891-5894.
22. Rohwedder, B., Mutti, Y., Dumy, P., and Mutter, M. (1998) Hydrazinolysis of Dde: Complete orthogonality with Aloc protecting groups. Tetrahedron Lett. 39, 1175-1178.
23. Spivey, A.C., and Maddaford, A. (1999) Synthetic methods part (v): Protecting groups. Annu Rep. Prog. Chem. Sect. B. 95, 83-95.
24. Taniguchi, T., and Ogasawara, K. Facile and specific nickel-catalyzed D-N-allylation. Tetrahedron Lett. 39, 4679-4682.