PCR based random mutagenesis approach for a defined DNA sequence using the mutagenic potential of oxidized nucleotide products

Open Biotechnology Journal

Volumn 5, Issue 1, 2011, Pages 21-27

  Mohan, Utpal ^a   Kaushik, Shubhangi ^a   Banerjee, Uttam Chand ^a  

^a NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH NIPER   (India)

Author keywords

Error prone PCR; Oxidized nucleotides; Random mutagenesis

Indexed keywords

DIRECTED EVOLUTION; DIVERSE RANGE; ERROR PRONE PCR; FERROUS SULPHATE; GENE SEQUENCES; IN-VITRO; MAGNESIUM IONS; MUTATION FREQUENCY; MUTATION SPECTRA; OPTIMIZED PARAMETER; OXIDIZED PRODUCTS; OXIDIZING AGENTS; OXIDIZING CONDITIONS; PCR AMPLIFICATION; RANDOM MUTAGENESIS; RANDOM MUTATION;

GENES; IRON; MAGNESIUM; MANGANESE; METAL IONS; MIXTURES; MUTAGENESIS; NUCLEOTIDES; OPTIMIZATION; PURIFICATION;

POLYMERASE CHAIN REACTION;

DEOXYADENOSINE TRIPHOSPHATE; DEOXYCYTIDINE TRIPHOSPHATE; DEOXYGUANOSINE TRIPHOSPHATE; FERROUS SULFATE; MAGNESIUM CHLORIDE; MANGANESE; THYMIDINE TRIPHOSPHATE;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; INCUBATION TIME; MUTAGENESIS; NUCLEIC ACID AMPLIFICATION; OXIDATION; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PURIFICATION;

EID: 80053591367     PISSN: None     EISSN: 18740707     Source Type: Journal    
DOI: 10.2174/1874070701105010021     Document Type: Article

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