Systematic selection of housekeeping genes for gene expression normalization in chicken embryo fibroblasts infected with Newcastle disease virus

Biochemical and Biophysical Research Communications

Volumn 413, Issue 4, 2011, Pages 537-540

  Yin, Renfu ^a,b,c   Liu, Xinxin ^a   Liu, Chang ^d   Ding, Zhuang ^a   Zhang, Xiaodong ^a   Tian, Furong ^c   Liu, Weihong ^a   Yu, Jinghai ^a   Li, Lin ^e   Hrabé de Angelis, Martin ^b,c   Stoeger, Tobias ^c  

^a JILIN UNIVERSITY   (China)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

^d YANBIAN UNIVERSITY   (China)

^e Institute of Animal Husbandry and Veterinary Science of Jilin   (China)

Author keywords

Chicken embryo fibroblasts; Housekeeping gene; Newcastle disease virus; QRT PCR

Indexed keywords

RNA 18S;

ANIMAL CELL; ARTICLE; CHICK EMBRYO; CONTROLLED STUDY; DISEASE COURSE; EMBRYO; FIBROBLAST; GENE EXPRESSION; GENETIC TRANSCRIPTION; HOUSEKEEPING GENE; NEWCASTLE DISEASE PARAMYXOVIRUS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS INFECTION;

ANIMALS; CHICK EMBRYO; FIBROBLASTS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); NEWCASTLE DISEASE; NEWCASTLE DISEASE VIRUS; POULTRY; REFERENCE STANDARDS; RNA, RIBOSOMAL, 18S; TRANSCRIPTION, GENETIC;

NEWCASTLE DISEASE VIRUS;

EID: 80053583557     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.08.131     Document Type: Article

References (15)

1. Saif Y.M., Barnes H.J. Diseases of poultry 2008, Blackwell Publishing Professional, Ames, Iowa. 12th ed.
2. Sinkovics J.G., Horvath J.C. Newcastle disease virus (NDV): brief history of its oncolytic strains. J. Clin. Virol. 2000, 16:1-15.
3. Huggett J., Dheda K., Bustin S., Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005, 6:279-284.
4. Ferguson B.S., Nam H., Hopkins R.G., Morrison R.F. Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes. PLoS One 2010, 5:e15208.
5. Gorzelniak K., Janke J., Engeli S., Sharma A.M. Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes. Horm. Metab. Res. 2001, 33:625-627.
6. Yin R., Ding Z., Liu X., Mu L., Cong Y., Stoeger T. Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts. J. Virol. Methods 2010, 167:107-111.
7. Yin R., Tian F., Frankenberger B., de Angelis M.H., Stoeger T. Selection and evaluation of stable housekeeping genes for gene expression normalization in carbon nanoparticle-induced acute pulmonary inflammation in mice. Biochem. Biophys. Res. Commun. 2010, 399:531-536.
8. Chitra P., Bakthavatsalam B., Palvannan T. Beta-2 microglobulin as an immunological marker to assess the progression of human immunodeficiency virus infected patients on highly active antiretroviral therapy. Clin. Chim. Acta 2011, 412:1151-1154.
9. Balenovic M., Savic V., Ekert Kabalin A., Jurinovic L., Ragland W.L. Abundance of IFN-alpha and IFN-gamma gene transcripts and absence of IL-2 transcripts in the blood of chickens vaccinated with live or inactivated NDV. Acta Vet. Hung. 2011, 59:141-148.
10. Schwarz H., Harlin O., Ohnemus A., Kaspers B., Staeheli P. Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay. J. Interferon Cytokine Res. 2004, 24:179-184.
11. Hoss A., Zwarthoff E.C., Zawatzky R. Differential expression of interferon alpha and beta induced with Newcastle disease virus in mouse macrophage cultures. J. Gen. Virol. 1989, 70(Pt 3):575-589.
12. Dheda K., Huggett J.F., Chang J.S., Kim L.U., Bustin S.A., Johnson M.A., Rook G.A., Zumla A. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal. Biochem. 2005, 344:141-143.
13. Demidenko N.V., Logacheva M.D., Penin A.A. Selection and validation of reference genes for quantitative real-time PCR in buckwheat (Fagopyrum esculentum) based on transcriptome sequence data. PLoS One 2011, 6:e19434.
14. Wierschke S., Gigout S., Horn P., Lehmann T.N., Dehnicke C., Brauer A.U., Deisz R.A. Evaluating reference genes to normalize gene expression in human epileptogenic brain tissues. Biochem. Biophys. Res. Commun. 2010, 403:385-390.
15. Maess M.B., Sendelbach S., Lorkowski S. Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages. BMC Mol. Biol. 2010, 11:90.