Genetic and quantitative assessment of Vibrio vulnificus populations in oyster (Crassostrea virginica) tissues

Environmental Microbiology Reports

Volumn 3, Issue 5, 2011, Pages 543-549

  Staley, Christopher ^a   Jones, Melissa K ^b   Wright, Anita C ^b   Harwood, Valerie J ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRASSOSTREA VIRGINICA; OSTREIDAE; VIBRIO VULNIFICUS;

EID: 80053519912     PISSN: None     EISSN: 17582229     Source Type: Journal    
DOI: 10.1111/j.1758-2229.2011.00256.x     Document Type: Article

References (31)

1. Aznar, R., Ludwig, W., Amann, R.I., and Schleifer, K.H. (1994) Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes. Int J Syst Bacteriol 44: 330-337.
2. Badgley, B.D., Nayak, B.S., and Harwood, V.J. (2010) The importance of sediment and submerged aquatic vegetation as potential habitats for persistent strains of enterococci in a subtropical watershed. Water Res 44: 5857-5866.
3. Brownell, M.J., Harwood, V.J., Kurz, R.C., McQuaig, S.M., Lukasik, J., and Scott, T.M. (2007) Confirmation of putative stormwater impact on water quality at a Florida beach by microbial source tracking methods and structure of indicator organism populations. Water Res 41: 3747-3757.
4. Buchrieser, C., Gangar, V.V., Murphree, R.L., Tamplin, M.L., and Kaspar, C.W. (1995) Multiple Vibrio vulnificus strains in oysters as demonstrated by clamped homogeneous electric field gel electrophoresis. Appl Environ Microbiol 61: 1163-1168.
5. Chatzidaki-Livanis, M., Hubbard, M.A., Gordon, K., Harwood, V.J., and Wright, A.C. (2006) Genetic distinctions among clinical and environmental strains of Vibrio vulnificus. Appl Environ Microbiol 72: 6136-6141.
6. Cohen, A.L.V., Oliver, J.D., DePaola, A., Feil, E.J., and Boyd, E.F. (2007) Emergence of a virulent clade of Vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island. Appl Environ Microbiol 73: 5553-5565.
7. DePaola, A., McLeroy, S., and McManus, G. (1997a) Distribution of Vibrio vulnificus phage in oyster tissues and other estuarine habitats. Appl Environ Microbiol 63: 2464-2467.
8. DePaola, A., Motes, M.L., Cook, D.W., Veazey, J., Garthright, W.E., and Blodgett, R. (1997b) Evaluation of an alkaline phosphatase-labeled DNA probe for enumeration of Vibrio vulnificus in Gulf Coast oysters. J Microbiol Methods 29: 115-120.
9. DePaola, A., Nordstrom, J.L., Dalsgaard, A., Forslund, A., Oliver, J., Bates, T., etal. (2003) Analysis of Vibrio vulnificus from market oysters and septicemia cases for virulence markers. Appl Environ Microbiol 69: 4006-4011.
10. DePaola, A., Jones, J.L., Noe, K.E., Byars, R.H., and Bowers, J.C. (2009) Survey of postharvest-processed oysters in the United States for levels of Vibrio vulnificus and Vibrio parahaemolyticus. J Food Prot 72: 2110-2113.
11. FDACFSAN (2009) Naturally Occurring Pathogens. National Shellfish Sanitation Program: Guide for Control of Molluscan Shellfish [WWW document]. URL
12. Galtsoff, P.S. (1964) The American oyster, Crassostrea virginica Gmelin. Fish B-NOAA 64: 1-480.
13. Gordon, K.V., Vickery, M.C., DePaola, A., Staley, C., and Harwood, V.J. (2008) Real-time PCR assays for quantification and differentiation of Vibrio vulnificus strains in oysters and water. Appl Environ Microbiol 74: 1704-1709.
14. Hlady, W.G., Mullen, R.C., and Hopkin, R.S. (1993) Vibrio vulnificus from raw oysters. Leading cause of reported deaths from food-borne illness in Florida. J Fla Med Assoc 80: 536-538.
15. Interstate Shellfish Sanitation Conference (2003) Issue relating to a Vibrio vulnificus risk management plan for oysters. In ISSC. Columbia, SC.
16. Jackson, J.K., Murphree, R.L., and Tamplin, M.L. (1997) Evidence that mortality from Vibrio vulnificus infection results from single strains among heterogeneous populations in shellfish. J Clin Microbiol 35: 2098-2101.
17. Kaysner, C.A., and DePaola, A. (2004) Vibrio. FDA Bacteriological Analytical Manual [WWW document]. URL
18. Koeuth, T., Versalovic, J., and Lupski, J.R. (1995) Differential subsequence conservation of interspersed repetitive Streptococcus pneumoniae BOX elements in diverse bacteria. Genome Res 5: 408-418.
19. Kogan, S.C., Doherty, M., and Gitschier, J. (1987) An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A. N Engl J Med 317: 985-990.
20. Kuhn, I., Allestam, G., Stenstrom, T.A., and Mollby, R. (1991) Biochemical fingerprinting of water coliform bacteria, a new method for measuring phenotypic diversity and for comparing different bacterial populations. Appl Environ Microbiol 57: 3171-3177.
21. Lin, M.L., and Schwarz, J.R. (2003) Seasonal shifts in population structure of Vibrio vulnificus in an estuarine environment as revealed by partial 16S ribosomal DNA sequencing. FEMS Microbiol Ecol 45: 23-27.
22. Malathum, K., Singh, K.V., Weinstock, G.M., and Murray, B.E. (1998) Repetitive sequence-based PCR versus pulsed-field gel electrophoresis for typing of Enterococcus faecalis at the subspecies level. J Clin Microbiol 36: 211-215.
23. Nilsson, W.B., Paranjype, R.N., DePaola, A., and Strom, M.S. (2003) Sequence polymorphism of the 16S rRNA gene of Vibrio vulnificus is a possible indicator of strain virulence. J Clin Microbiol 41: 442-446.
24. Rosche, T.M., Yano, Y., and Oliver, J.D. (2005) A rapid and simple PCR analysis indicates there are two subgroups of Vibrio vulnificus which correlate with clinical or environmental isolation. Microbiol Immunol 49: 381-389.
25. Staley, C., and Harwood, V.J. (2007) Characterization of a putative virulence marker in Vibrio vulnificus unique to clinically-associated strains. In 107th General Meeting of the American Society for Microbiology. Toronto, Canada.
26. Tamplin, M.L., and Capers, G.M. (1992) Persistence of Vibrio vulnificus in tissues of Gulf Coast oysters, Crassostrea virginica, exposed to seawater disinfected with UV light. Appl Environ Microbiol 58: 1506-1510.
27. Tamplin, M.L., Jackson, J.K., Buchrieser, C., Murphree, R.L., Portier, K.M., Gangar, V., etal. (1996) Pulsed-field gel electrophoresis and ribotype profiles of clinical and environmental Vibrio vulnificus isolates. Appl Environ Microbiol 62: 3572-3580.
28. Versalovic, J., Koeuth, T., and Lupski, J.R. (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19: 6823-6831.
29. Vickery, M.C., Nilsson, W.B., Strom, M.S., Nordstrom, J.L., and Depaola, A. (2007) A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus. J Microbiol Methods 68: 376-384.
30. Wright, A.C., Miceli, G.A., Landry, W.L., Christy, J.B., Watkins, W.D., and Morris, J.G., Jr (1993) Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe. Appl Environ Microbiol 59: 541-546.
31. Wright, A.C., Garrido, V., Debuex, G., Farrell-Evans, M., Mudbidri, A.A., and Otwell, W.S. (2007) Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria. Appl Environ Microbiol 73: 7477-7481.